{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:03:26Z","timestamp":1792623806251,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,9,6]],"date-time":"2025-09-06T00:00:00Z","timestamp":1757116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["543002001"],"award-info":[{"award-number":["543002001"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ultrasound in Obstet &amp; Gyne"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the performance of non\u2010invasive prenatal testing (NIPT) in vanishing\u2010twin and multiple pregnancies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study was conducted as part of the TRIDENT\u20102 study, in which NIPT was offered as a first\u2010tier screening test to women with a multiple pregnancy or vanishing\u2010twin pregnancy between 1 June 2020 and 31 March 2023 in The Netherlands. Abnormal NIPT results were investigated by follow\u2010up invasive prenatal testing and\/or postnatal genetic testing. Chorionicity, amnionicity and type of vanishing twin were determined on first\u2010trimester ultrasound examination. A vanishing twin was classified as Type\u2009I in the presence of an additional empty gestational sac or as Type\u2009II when an additional non\u2010viable embryo was observed on ultrasound. The performance of NIPT (sensitivity, specificity and positive predictive value (PPV)) was assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      NIPT was performed in 655 women with a vanishing\u2010twin pregnancy, of which 231 were Type\u2009I, 374 were Type\u2009II and 50 were of unknown type. Women with a Type\u2010II vanishing\u2010twin pregnancy had a significantly higher likelihood of receiving an abnormal NIPT result compared to those with Type\u2009I (12.6%\n                      <jats:italic>vs<\/jats:italic>\n                      1.7%;\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.001). Among the 655 vanishing twins, NIPT was indicative of trisomies 21, 18 and 13 in 17 cases (screen\u2010positive rate (SPR), 2.60%), four cases (SPR, 0.61%) and eight cases (SPR, 1.22%), respectively. NIPT was indicative of additional findings (chromosomal aberrations other than the major trisomies) in 29 cases that underwent genome\u2010wide NIPT (SPR, 6.16%). In 7\/17 (41.2%) cases, trisomy 21 was confirmed in the remaining fetus by cytogenetic follow\u2010up. The sensitivity of NIPT for the detection of trisomy 21 in vanishing\u2010twin pregnancies was 100% (95%\u2009CI, 59.0\u2013100%), the specificity was 98.5% (95%\u2009CI, 97.2\u201399.3%) and the PPV was 41.2% (95%\u2009CI, 18.4\u201367.1%). None of the cases of trisomy 18 (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20094), trisomy 13 (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098) or with additional findings (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200929) were confirmed in the remaining fetus. Of the 12 cases in which NIPT was performed after\u200915\u2009weeks' gestation, there were no discordant\u2010positive results. NIPT was performed in 2992 women with a dichorionic diamniotic twin pregnancy, 1112 women with a monochorionic twin pregnancy and 75 women with a triplet pregnancy. Of the 2992 dichorionic twin pregnancies, 27 NIPT results were indicative of trisomy 21, 18 or 13 (SPR, 0.90%), of which 21 were confirmed in one fetus. In addition, 16 NIPT results were indicative of an additional finding (SPR, 0.75%), of which three were confirmed by invasive prenatal testing. In 3\/1112 (0.3%) monochorionic twin pregnancies, NIPT was indicative of trisomy 21, which was confirmed in both fetuses in all cases. In addition, NIPT was indicative of an additional finding in four cases (SPR, 0.49%), of which none were confirmed. Of the 75 triplet pregnancies, NIPT was indicative of trisomy 21 in one case (SPR, 1.33%); trisomy 21 was confirmed in one of the three triplet fetuses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Women with a Type\u2010II vanishing\u2010twin pregnancy are more likely to receive an abnormal NIPT result compared to those with a Type\u2010I vanishing\u2010twin pregnancy. NIPT appears suitable for detecting trisomy 21 in the remaining fetus of a vanishing\u2010twin pregnancy, however, none of the trisomy 18, trisomy 13 or additional findings could be confirmed on cytogenetic follow\u2010up. There were no discordant\u2010positive results reported when NIPT was conducted after\u200915\u2009weeks' gestation. \u00a9 2025 The Author(s).\n                      <jats:italic>Ultrasound in Obstetrics &amp; Gynecology<\/jats:italic>\n                      published by John Wiley &amp; Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/uog.70015","type":"journal-article","created":{"date-parts":[[2025,9,6]],"date-time":"2025-09-06T21:19:48Z","timestamp":1757193588000},"page":"738-746","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Performance of non\u2010invasive prenatal testing in vanishing\u2010twin and multiple pregnancies: results of\n                    <scp>TRIDENT<\/scp>\n                    \u20102 study"],"prefix":"10.1002","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8088-6176","authenticated-orcid":false,"given":"J. C. A.","family":"van Eekhout","sequence":"first","affiliation":[{"name":"Department of Clinical Genetics, Erasmus MC University Medical Centre  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. J.","family":"Bax","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Amsterdam UMC University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. van Prooyen","family":"Schuurman","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics, Erasmus MC University Medical Centre  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. C.","family":"Becking","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University Medical Center Utrecht, Utrecht University  Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. J. E. M.","family":"van der Ven","sequence":"additional","affiliation":[{"name":"Verloskundigenpraktijk Velp  Velp The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Van Opstal","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics, Erasmus MC University Medical Centre  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. M. J.","family":"Boon","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Amsterdam UMC  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. V. E.","family":"Macville","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics, GROW School for Oncology and Reproduction Maastricht University Medical Centre  Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7372-4291","authenticated-orcid":false,"given":"M. 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