{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T08:58:56Z","timestamp":1782637136358,"version":"3.54.5"},"reference-count":64,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,1,15]],"date-time":"2022-01-15T00:00:00Z","timestamp":1642204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      A cross\u2010sectional study was carried out to assess the prevalence of\n                      <jats:italic>Mycoplasma hyopneumoniae<\/jats:italic>\n                      infections before vaccination in 3\u2010week\u2010old piglets and to gain information about infection dynamics.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In 13 German and three Austrian farms with a known history of enzootic pneumonia, 790 piglets and 158 sows were sampled (blood samples, tracheobronchial swabs [TBS] [piglets], laryngeal swabs [LS] [sows]), and 525 pen\u2010based oral fluids (OFs) were collected in growing and fattening pigs. Laboratory diagnostics included enzyme\u2010linked immunosorbent assay (ELISA) and real\u2010time polymerase chain reaction (PCR) analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Antibodies to\n                      <jats:italic>M. hyopneumoniae<\/jats:italic>\n                      were present in 87.5 per cent of all herds. Seroprevalence ranged from 0.0 to 100.0 per cent and 0.0 to 88.0 per cent in sows and piglets, respectively.\n                      <jats:italic>M. hyopneumoniae<\/jats:italic>\n                      \u2010deoxyribonucleic acid (DNA) was present in 3.8 and 0.4 per cent of LS and TBS, respectively. Gilts had a 10.9 times higher chance being\n                      <jats:italic>M. hyopneumoniae<\/jats:italic>\n                      PCR\u2010positive than older sows. In 75.0 per cent of all farms,\n                      <jats:italic>M. hyopneumoniae<\/jats:italic>\n                      \u2010DNA was present in OFs. Detection rate was significantly higher in OFs of 20\u2010week\u2010old than in younger pigs (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Results indicate that\n                      <jats:italic>M. hyopneumoniae<\/jats:italic>\n                      infections of the lower respiratory tract in piglets are rare but highlight the role of gilts in maintaining infection in the herd. Collecting OFs seems promising for surveillance, if coughing occurs simultaneously.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vetr.1317","type":"journal-article","created":{"date-parts":[[2022,1,15]],"date-time":"2022-01-15T13:22:56Z","timestamp":1642252976000},"source":"Crossref","is-referenced-by-count":16,"title":["Cross\u2010sectional study on the in\u2010herd prevalence of\n                    <i>Mycoplasma hyopneumoniae<\/i>\n                    at different stages of pig production"],"prefix":"10.1002","volume":"191","author":[{"given":"Pauline","family":"Deffner","sequence":"first","affiliation":[{"name":"Clinic for Swine Centre for Clinical Veterinary Medicine, LMU Munich  Oberschlei\u00dfheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Maurer","sequence":"additional","affiliation":[{"name":"Clinic for Swine Centre for Clinical Veterinary Medicine, LMU Munich  Oberschlei\u00dfheim 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