{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T20:35:46Z","timestamp":1782333346381,"version":"3.54.5"},"reference-count":19,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,2,19]],"date-time":"2021-02-19T00:00:00Z","timestamp":1613692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2021,5]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Bovine leptospirosis is mainly characterized by reproductive disorders. Contamination of the oocyte was previously demonstrated\n in vitro<\/jats:italic>\n , resulting in some apparent damage. However, it is not clear whether it occurs under natural conditions. The present study aimed to characterize the presence of pathogenic\n Leptospira<\/jats:italic>\n DNA in the ovarian follicles of non\u2010pregnant cows.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Follicular fluid samples were collected from 65 animals and subjected to\n lip<\/jats:italic>\n L32 PCR and\n sec<\/jats:italic>\n Y sequencing.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n In total, seven of 65 (10.8%) were positive, indicating a possible early infection of the oocyte. Moreover,\n sec<\/jats:italic>\n Y sequencing identified\n L. interrogans<\/jats:italic>\n and\n L. santarosai<\/jats:italic>\n , both very closely related to bovine strains from the Sejroe serogroup (Hardjoprjitno and Guaricura). We demonstrated that ovarian follicles can also be infected.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n It was hypothesised that ovarian infection can contribute to embryonic death, causing reproductive failure and estrus repetition. In the present study, we show that the organism identified in the follicle is closely related to one that is known to be associated with reproductive disorders.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/vetr.143","type":"journal-article","created":{"date-parts":[[2021,2,19]],"date-time":"2021-02-19T15:41:08Z","timestamp":1613749268000},"source":"Crossref","is-referenced-by-count":19,"title":["Characterization of leptospiral DNA in the follicular fluid of non\u2010pregnant cows"],"prefix":"10.1002","volume":"188","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9882-3130","authenticated-orcid":false,"given":"Maria Isabel Nogueira","family":"Di Azevedo","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Bacteriology Biomedical Institute Niter\u00f3i Fluminense Federal University Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno Cabral","family":"Pires","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Bacteriology Biomedical Institute Niter\u00f3i Fluminense Federal University Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Figueiredo Cardoso","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Bacteriology Biomedical Institute Niter\u00f3i Fluminense Federal University Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filipe Anibal","family":"Carvalho\u2010Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Molecular Systematics Oswaldo Cruz Institute Oswaldo Cruz Foundation Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter","family":"Lilenbaum","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Bacteriology Biomedical Institute Niter\u00f3i Fluminense Federal University Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,19]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"99","article-title":"Animal leptospirosis","volume":"387","author":"Ellis WA","year":"2015","journal-title":"Cur Top Microbiol Immunol"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-018-1604-9"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.09.011"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.118.1.11"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2017.01.008"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.2460\/ajvr.1986.47.08.1694","article-title":"Isolation of leptospires from the genital tracts of Iowa cows","volume":"47","author":"Ellis WA","year":"1986","journal-title":"Am J Vet 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