{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T03:39:02Z","timestamp":1783741142790,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:00:00Z","timestamp":1733875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2024,12,14]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Bovine leukaemia virus (BLV)\u2010infected Holstein cattle carrying certain bovine leukocyte antigen (\n BoLA)\u2010DRB3<\/jats:italic>\n alleles were previously shown to be resistant to BLV provirus multiplication, while those carrying other alleles were susceptible. This study aimed to determine whether the\n BoLA\u2010DRB3<\/jats:italic>\n alleles carried by BLV\u2010infected cattle could predict proviral load (PVL) and peripheral blood lymphocyte (PBL) count distribution (PVL\/PBL distribution).\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Blood samples from Holstein cattle on four dairy farms were tested for the presence of BLV antibodies using a commercial ELISA. The PVL and PBL levels of the BLV\u2010infected cattle were also measured, and genotyping was performed to identify the\n BoLA\u2010DRB3<\/jats:italic>\n alleles they carried, impact of the various\n BoLA\u2010DRB3<\/jats:italic>\n alleles on the PVL\/PBL distribution was then investigated.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Of the 316 cattle tested, 114 were positive for BLV. BLV\u2010infected cattle carrying\n BoLA\u2010DRB3<\/jats:italic>\n alleles\n DRB3*009<\/jats:italic>\n :\n 02<\/jats:italic>\n ,\n DRB3<\/jats:italic>\n *002<\/jats:italic>\n :\n 01<\/jats:italic>\n and DRB3\n *014<\/jats:italic>\n :\n 01<\/jats:italic>\n :\n 01<\/jats:italic>\n were classified as resistant (\n n<\/jats:italic>\n \u00a0=\u00a043), those carrying\n DRB3*012<\/jats:italic>\n :\n 01<\/jats:italic>\n and\n DRB3<\/jats:italic>\n *015<\/jats:italic>\n :\n 01<\/jats:italic>\n alleles were classified as susceptible (\n n<\/jats:italic>\n \u00a0=\u00a042) and the remaining cattle were classified as nonsusceptible\/nonresistant (\n n<\/jats:italic>\n \u00a0=\u00a029). Multiple regression analysis revealed that PVL was positively correlated (\n p<\/jats:italic>\n = 2.1\u00a0\u00d7\u00a010\n \u221223<\/jats:sup>\n ) with PBL count and age was negatively correlated (\n p<\/jats:italic>\n \u00a0=\u00a01.9\u00a0\u00d7\u00a010\n \u22126<\/jats:sup>\n ) with PBL count. Cattle with\n DRB3*014<\/jats:italic>\n :\n 01<\/jats:italic>\n :\n 01<\/jats:italic>\n tended to have a lower PBL count (\n p<\/jats:italic>\n \u00a0=\u00a00.031).\n <\/jats:p>\n <\/jats:sec>\n \n Limitation<\/jats:title>\n \n The effects of the\n BoLA\u2010DRB3<\/jats:italic>\n alleles\n DRB3<\/jats:italic>\n *002:01,\n DRB3<\/jats:italic>\n *009:02,\n DRB3<\/jats:italic>\n *012:01 and\n DRB3<\/jats:italic>\n *015:01 on PVL\/PBL distribution were unclear due to the small numbers of BLV\u2010infected animals carrying these alleles.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n The BLV transmission risk in cattle can be estimated by examining their\n BoLA\u2010DRB3<\/jats:italic>\n alleles.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/vetr.4909","type":"journal-article","created":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T13:29:23Z","timestamp":1733923763000},"source":"Crossref","is-referenced-by-count":1,"title":["BoLA\u2010DRB3<\/i>\n alleles influence proviral load and peripheral blood lymphocyte distribution in bovine leukaemia virus\u2010infected cattle"],"prefix":"10.1002","volume":"195","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9304-3752","authenticated-orcid":false,"given":"Kenichiro","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Nagano Prefecture Saku Livestock Hygiene Service Center Saku Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Katakura","sequence":"additional","affiliation":[{"name":"Nagano Prefecture Matsumoto Livestock Hygiene Service Center Matsumoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yui","family":"Ito","sequence":"additional","affiliation":[{"name":"Nagano Prefecture Matsumoto Livestock Hygiene Service Center Matsumoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Kato","sequence":"additional","affiliation":[{"name":"Nagano Prefecture Matsumoto Livestock Hygiene Service Center Matsumoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiaki","family":"Goto","sequence":"additional","affiliation":[{"name":"Nagano Prefecture Ina Livestock Hygiene Service Center Ina Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Nagano Prefecture Saku Livestock Hygiene Service Center Saku Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,11]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(88)90066-1"},{"key":"e_1_2_12_3_1","first-page":"129","article-title":"Bovine enzootic leukosis","volume":"10","author":"Bendixen HJ","year":"1965","journal-title":"Adv Vet Sci."},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9121058"},{"issue":"7","key":"e_1_2_12_5_1","doi-asserted-by":"crossref","first-page":"705","DOI":"10.2460\/javma.1979.175.07.705","article-title":"Relationship between lymphosarcoma and persistent lymphocytosis in cattle: a review","volume":"175","author":"Ferrer JF","year":"1979","journal-title":"J Am Vet Med Assoc"},{"issue":"1","key":"e_1_2_12_6_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.2460\/ajvr.1985.46.01.123","article-title":"Role of insects in the transmission of bovine leukosis virus: potential for transmission by stable flies, horn flies, and tabanids","volume":"46","author":"Buxton BA","year":"1985","journal-title":"Am J Vet Res"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms1939.43.79"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-0720(15)30367-4"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6267692"},{"issue":"6","key":"e_1_2_12_10_1","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.2460\/ajvr.1981.42.06.1052","article-title":"In utero transmission of bovine leukemia virus","volume":"42","author":"Van der Maaten MJ","year":"1981","journal-title":"Am J Vet Res"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.45.3.1172-1176.1983"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5877(03)00057-6"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.12-0374"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2015.08.020"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2015-10089"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.244.8.914"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2007.05.010"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.1993.54.03.373"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2018-16186"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-7-91"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.68.11.1220"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.14-0624"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.17-0455"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.102464"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.8.5706-5710.1996"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0147-9571(99)00074-0"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1651"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2010.00208.x"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2012.09.005"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1900342"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10528-016-9712-6"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.151.12.6977"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-019-0732-1"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-8-167"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12977-019-0476-z"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci10040250"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002510000293"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2011.01708.x"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12030352"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2012.244"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003687"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10080987"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/vrc2.883"},{"key":"e_1_2_12_44_1","first-page":"365","article-title":"Factors affecting the infectivity of lymphocytes from cattle with bovine leukosis virus","volume":"48","author":"Buxton BA","year":"1984","journal-title":"Can J Comp Med."}],"container-title":["Veterinary Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vetr.4909","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T12:28:18Z","timestamp":1761568098000},"score":1,"resource":{"primary":{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/10.1002\/vetr.4909"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,11]]},"references-count":43,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12,14]]}},"alternative-id":["10.1002\/vetr.4909"],"URL":"https:\/\/doi.org\/10.1002\/vetr.4909","archive":["Portico"],"relation":{},"ISSN":["0042-4900","2042-7670"],"issn-type":[{"value":"0042-4900","type":"print"},{"value":"2042-7670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,11]]},"article-number":"e4909"}}