{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:09:52Z","timestamp":1789193392782,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2026,5,2]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>This study measured serum biomarkers at baseline and during an ovariohysterectomy in healthy female Spanish greyhounds, aiming to enhance the objectivity of current methods for assessing acute postoperative pain in dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Blood samples from 20 Spanish greyhounds were collected before drug administration (T0\u2010baseline). Premedication included dexmedetomidine and methadone, followed by propofol and isoflurane. Four additional samples were taken: post\u2010second ovariectomy (T1\u2010PostOvary), 4\u00a0hours post\u2010premedication (T2\u2010PreRescue), 30\u00a0minutes post\u2010rescue methadone (T3\u2010PostRescue) and 24\u00a0hours post\u2010extubation (T4\u201024H). Biomarkers were quantified via ELISA, and postoperative pain was assessed using the short\u2010form Glasgow scale (Glasgow\u2010SF). Data analysis included ANOVA, Friedman test and Spearman's correlation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Glutamate (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.007; Kendall's\n                      <jats:italic>W<\/jats:italic>\n                      = 0.186) and calcitonin gene\u2010related peptide (CGRP) (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.001;  = 0.324) peaked at T0\u2010baseline. Cortisol peaked at T3\u2010PostRescue (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.041;  = 0.099). Glasgow\u2010SF scores increased postoperatively compared with T0\u2010baseline (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.001; Kendall's\n                      <jats:italic>W<\/jats:italic>\n                      = 0.612), without significant biomarker correlation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Limitations<\/jats:title>\n                    <jats:p>Sample homogeneity may limit result generalisability and lack of acclimatisation may introduce bias, as initial stress could affect the synthesis of stress\u2010related biomarkers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Although glutamate, CGRP and cortisol levels fluctuated over time\u2014with glutamate and CGRP peaking at T0\u2010baseline\u2014none proved useful for assessing postoperative pain compared with conventional clinical parameters.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vetr.6023","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T12:45:43Z","timestamp":1764852343000},"source":"Crossref","is-referenced-by-count":0,"title":["Analysis of serum biomarkers associated with pain in Spanish greyhounds undergoing ovariohysterectomy"],"prefix":"10.1002","volume":"198","author":[{"given":"Rocio","family":"Navarrete\u2010Calvo","sequence":"first","affiliation":[{"name":"Department of Animal Medicine and Surgery Veterinary Faculty University of C\u00f3rdoba  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Andr\u00e9s","family":"Fern\u00e1ndez\u2010Sarmiento","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery Veterinary Faculty University of C\u00f3rdoba  C\u00f3rdoba 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