{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T10:55:57Z","timestamp":1790938557175,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:00:00Z","timestamp":1766707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:00:00Z","timestamp":1766707200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2026,10,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Despite common concerns, evidence that anaesthesia impairs vaccine efficacy remains limited. This study assessed the impact of general anaesthesia on the immune response to rabies vaccination in cats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Thirty\u2010five healthy female cats (6\u201312 months old) undergoing elective spaying were enrolled. Group 1 (\n                      <jats:italic>n<\/jats:italic>\n                      =\u00a025) received an inactivated, adjuvanted rabies vaccine at the end of anaesthesia; group 2 (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a011) was vaccinated outside the peri\u2010anaesthetic period. Rabies\u2010neutralising antibody titres were measured by the fluorescent antibody virus neutralisation test 1 month post\u2010vaccination in both groups. In group 1, immune gene expression (tumour necrosis factor\u2010\u03b1 [TNF\u2010\u03b1], interleukin\u20106 [IL\u20106] and C\u2012X\u2012C motif chemokine ligand 10 [CXCL10]) was assessed by qPCR following ex vivo blood stimulation with the vaccine solution.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All cats seroconverted with titres greater than 0.5 IU\/mL, with no significant difference between the groups. TNF\u2010\u03b1 expression was significantly upregulated after stimulation at both time points in group 1. No induction of IL\u20106 or CXCL10 was observed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Limitations<\/jats:title>\n                    <jats:p>This study used a single vaccine and a single anaesthetic protocol in a homogeneous group of healthy young cats. As such, the generalisability of the findings may be limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Under these controlled conditions, anaesthesia did not impair innate or adaptive immune responses to rabies vaccination. Simultaneous vaccination and anaesthesia may therefore be acceptable in contexts such as sterilisation campaigns in stray or aggressive cats.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vetr.70223","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T08:46:06Z","timestamp":1766738766000},"source":"Crossref","is-referenced-by-count":1,"title":["Impact of general anaesthesia on immune response to first rabies vaccination in seronegative domestic cats"],"prefix":"10.1002","volume":"199","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5657-0027","authenticated-orcid":false,"given":"Pierre","family":"Bessi\u00e8re","sequence":"first","affiliation":[{"name":"Univ Toulouse ENVT, INRAE, IHAP Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romane","family":"Breysse","sequence":"additional","affiliation":[{"name":"Univ Toulouse ENVT, INRAE, IHAP Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Chr\u00e9tien","sequence":"additional","affiliation":[{"name":"Univ Toulouse ENVT, INRAE, IHAP Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon","family":"Hayes","sequence":"additional","affiliation":[{"name":"Univ Toulouse ENVT, INRAE, IHAP Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9verine","family":"Boullier","sequence":"additional","affiliation":[{"name":"Univ Toulouse ENVT, INRAE, INTHERES Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anouck","family":"Labadie","sequence":"additional","affiliation":[{"name":"Nancy Laboratory for Rabies and Wildlife, ANSES, INTERFAS Unit  Malz\u00e9ville France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Rieder","sequence":"additional","affiliation":[{"name":"Nancy Laboratory for Rabies and Wildlife, ANSES, INTERFAS Unit  Malz\u00e9ville France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marine","family":"Wasniewski","sequence":"additional","affiliation":[{"name":"Nancy Laboratory for Rabies and Wildlife, ANSES, INTERFAS Unit  Malz\u00e9ville France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9raldine","family":"Jourdan","sequence":"additional","affiliation":[{"name":"Univ Toulouse ENVT, INSERM, CNRS, EFS, RESTORE Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(94)90004-3"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00540-008-0626-2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/vaa.12191"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/vaa.12125"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9592.2006.01897.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.2_12431"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.230.1.52"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.233.1.116"},{"key":"e_1_2_10_10_1","article-title":"American Society of Anesthesiologists physical status classification system","author":"Hendrix JM","year":"2025","journal-title":"StatPearls [Internet]"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902575"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2007.11.014"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-801919-1.00018-X"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.2307\/2533289"},{"key":"e_1_2_10_15_1","unstructured":"R Core Team.R: a language and environment for statistical computing. 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