{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T19:58:58Z","timestamp":1790539138463,"version":"4.1.0"},"reference-count":8,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Bovine leptospirosis is a zoonosis of global importance that is associated with reproductive losses. Although diagnosis in females has advanced, genital infection in males remains poorly understood. This cross\u2010sectional study aimed to compare diagnostic methods and sample types for detecting bovine genital leptospirosis (BGL) in bulls under field conditions.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Blood, urine, semen and preputial cytology samples were collected from 37 unvaccinated bulls and analysed by serology (microscopic agglutination test \u2010MAT) and\n lipL32<\/jats:italic>\n \u2010PCR.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Serology showed low seroreactivity (13.5%), whereas PCR detected infection in 80.5% of bulls.\n Leptospira<\/jats:italic>\n DNA was identified in semen (70.6%), preputial cytology samples (50%) and urine (46.8%). PCR detected a higher proportion of positive animals than MAT (\n p<\/jats:italic>\n < 0.05). The genital tract had a higher frequency of\n Leptospira<\/jats:italic>\n detection than the urinary tract (\n p<\/jats:italic>\n < 0.05), with no significant difference between semen and preputial cytology samples (\n p<\/jats:italic>\n > 0.05).\n <\/jats:p>\n <\/jats:sec>\n \n Limitations<\/jats:title>\n The sample size was small, and the number of samples collected differed among sample types.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n PCR using genital samples\u2014semen and\/or preputial cytology\u2014is the method of choice for diagnosing BGL in asymptomatic bulls.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/vetr.71220","type":"journal-article","created":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T15:40:58Z","timestamp":1790523658000},"source":"Crossref","is-referenced-by-count":0,"title":["Diagnosis of bovine genital leptospirosis in asymptomatic bulls: Comparison of methods and samples under field conditions"],"prefix":"10.1002","author":[{"given":"Juliana","family":"Pedrosa","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Bacteriology Biomedical Institute of Fluminense Federal University Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiany","family":"Motta","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Bacteriology Biomedical Institute of Fluminense Federal University Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilmara","family":"Gregg","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Bacteriology Biomedical Institute of Fluminense Federal University Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Faculdade de Veterin\u00e1ria Fluminense Federal University Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter","family":"Lilenbaum","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Bacteriology Biomedical Institute of Fluminense Federal University Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani14020322"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.09.011"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","DOI":"10.1590\/1678-5150-pvb-7376","article-title":"Bovine genital leptospirosis: findings in bulls in caatinga biome conditions","volume":"43","author":"Barnab\u00e9 NNC","year":"2023","journal-title":"Pesquisa Vet Brasil"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2022.105841"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2025.108088"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14747"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/vetr.3309"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-013-9582-x"}],"container-title":["Veterinary Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vetr.71220","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vetr.71220","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vetr.71220","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T19:54:53Z","timestamp":1790538893000},"score":1,"resource":{"primary":{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/10.1002\/vetr.71220"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,27]]},"references-count":8,"alternative-id":["10.1002\/vetr.71220"],"URL":"https:\/\/doi.org\/10.1002\/vetr.71220","archive":["Portico"],"relation":{},"ISSN":["0042-4900","2042-7670"],"issn-type":[{"value":"0042-4900","type":"print"},{"value":"2042-7670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,27]]},"article-number":"e71220"}}