{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T02:05:54Z","timestamp":1790820354004,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Equine viral arteritis (EVA) is a contagious disease that affects equine reproduction and international trade. Despite its importance, epidemiological data from Spain are limited. This study aimed to assess EVA seroprevalence and evaluate the diagnostic performance of a commercial ELISA in horses before export.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n From 2016 to 2023, 5000 serum samples from horses across 16 Spanish autonomous communities were tested using a commercial indirect ELISA. Samples with inconclusive results, or those submitted with specific requests, underwent virus neutralisation testing (VNT). Diagnostic performance was assessed using receiver operating characteristic curve analysis and the kappa coefficient.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The apparent seroprevalence was 13.3% (664\/5000), and the Rogan\u2013Gladen\u2010adjusted seroprevalence was 2.3%. Apparent seroprevalence decreased over time, and adjusted estimates reached 0.0% from 2020 onwards. ELISA showed 98.9% sensitivity and 88.7% specificity, with substantial agreement with the VNT (\n \u03ba<\/jats:italic>\n \u00a0=\u00a00.68). Seroprevalence increased with age and was highest in Andalusia and Castile and Leon.\n <\/jats:p>\n <\/jats:sec>\n \n Limitations<\/jats:title>\n The study included only horses undergoing pre\u2010export testing and was dominated by Spanish purebred horses; therefore, findings may not represent the wider Spanish equine population.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n The declining EVA seroprevalence among horses undergoing pre\u2010export testing may reflect improved control measures. ELISA may be suitable for pre\u2010export screening, with VNT confirmation recommended for values above 37.14%.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/vetr.71223","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:37:26Z","timestamp":1790818646000},"source":"Crossref","is-referenced-by-count":0,"title":["Equine viral arteritis in horses undergoing pre\u2010export testing in Spain (2016\u20132023): Seroprevalence estimates and diagnostic performance of a commercial indirect ELISA"],"prefix":"10.1002","author":[{"given":"Paloma","family":"Gago","sequence":"first","affiliation":[{"name":"VISAVET Health Surveillance Centre Universidad Complutense de Madrid Madrid Spain"},{"name":"Department of Animal Health, Faculty of Veterinary Medicine Universidad Complutense de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abel","family":"Dorrego","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre Universidad Complutense de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"Pozo","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre Universidad Complutense de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"Raez","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre Universidad Complutense de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belen","family":"Rivera","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre Universidad Complutense de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc\u00eda","family":"de Juan","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre Universidad Complutense de Madrid Madrid Spain"},{"name":"Department of Animal Health, Faculty of Veterinary Medicine Universidad Complutense de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1142-3944","authenticated-orcid":false,"given":"Fatima","family":"Cruz\u2010Lopez","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre Universidad Complutense de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"3","article-title":"Isolation of a filterable agent causing arteritis of horses and abortion by mares; its differentiation from the equine abortion (influenza) virus","volume":"47","author":"Bryans JT","year":"1957","journal-title":"Cornell Vet"},{"key":"e_1_2_10_3_1","first-page":"69","article-title":"An outbreak of abortion caused by the equine arteritis virus","volume":"47","author":"Bryans JT","year":"1957","journal-title":"Cornell Vet"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/S0749-0739(17)30397-8","article-title":"Equine viral arteritis","volume":"9","author":"Timoney PJ","year":"1993","journal-title":"Vet Clin N Am Equine Pract."},{"key":"e_1_2_10_5_1","first-page":"189","article-title":"Transmission of the South African asinine strain of equine arteritis virus (EAV) among horses and between donkeys and horses","volume":"63","author":"Paweska JT","year":"1996","journal-title":"Onderstepoort J Vet Res"},{"key":"e_1_2_10_6_1","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.cveq.2014.08.011","article-title":"Equine viral arteritis","volume":"30","author":"Balasuriya UBR","year":"2014","journal-title":"Vet Clin N Am Equine Pract."},{"key":"e_1_2_10_7_1","doi-asserted-by":"crossref","first-page":"596","DOI":"10.2746\/042516403775467162","article-title":"Lateral transmission of equine arteritis virus among Lipizzaner stallions in South Africa","volume":"35","author":"Guthrie AJ","year":"2003","journal-title":"Equine Vet J"},{"key":"e_1_2_10_8_1","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1111\/j.2042-3306.1997.tb01666.x","article-title":"Equine viral arteritis in newborn foals: clinical, pathological, serological, microbiological and immunohistochemical observations","volume":"29","author":"Del Piero F","year":"1997","journal-title":"Equine Vet J."},{"key":"e_1_2_10_9_1","first-page":"523","article-title":"Natural equine viral arteritis in foals","volume":"123","author":"Golnik W","year":"1981","journal-title":"Schweiz Arch Tierheilkd."},{"key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"769","DOI":"10.2460\/javma.1986.189.07.769","article-title":"Transmissibility and abortogenic effect of equine viral arteritis in mares","volume":"189","author":"Cole JR","year":"1986","journal-title":"J Am Vet Med Assoc."},{"key":"e_1_2_10_11_1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/j.2042-3306.1992.tb02803.x","article-title":"Fatal, congenitally acquired infection with equine arteritis virus in a neonatal Thoroughbred","volume":"24","author":"Vaala WE","year":"1992","journal-title":"Equine Vet J."},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/S0034-5288(18)30616-7","article-title":"Demonstration of the carrier state in naturally acquired equine arteritis virus infection in the stallion","volume":"41","author":"Timoney PJ","year":"1986","journal-title":"Res Vet Sci."},{"key":"e_1_2_10_13_1","unstructured":"Ministerio de Agricultura Alimentaci\u00f3n y Medio Ambiente. Real Decreto 526\/2014 de 20 de junio por el que se establece la lista de las enfermedades de los animales de declaraci\u00f3n obligatoria y se regula su notificaci\u00f3n. Bolet\u00edn Oficial del Estado; 2014. Available from: Accessed June 8 2026.www.boe.es\/eli\/es\/rd\/2014\/06\/20\/526"},{"key":"e_1_2_10_14_1","first-page":"21","article-title":"Commission Implementing Regulation (EU) 2018\/1882 of 3 December 2018 on the application of certain disease prevention and control rules to categories of listed diseases and establishing a list of species and groups of species posing a considerable risk for the spread of those listed diseases","volume":"308","author":"European Commission","year":"2018","journal-title":"Off J Eur Union"},{"key":"e_1_2_10_15_1","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.2460\/ajvr.1981.42.09.1501","article-title":"Stability of viability and immunizing potency of lyophilized, modified equine arteritis live\u2010virus vaccine","volume":"42","author":"Harry TO","year":"1981","journal-title":"Am J Vet Res."},{"key":"e_1_2_10_16_1","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.2460\/ajvr.1981.42.07.1218","article-title":"Pathologic features of horses given avirulent equine arteritis virus intramuscularly","volume":"42","author":"McCollum WH","year":"1981","journal-title":"Am J Vet Res."},{"key":"e_1_2_10_17_1","unstructured":"Ministerio de Agricultura Pesca y Alimentaci\u00f3n.Real Decreto 779\/2023 de 10 de octubre por el que se establece la comunicaci\u00f3n de enfermedades de los animales de declaraci\u00f3n obligatoria y se regula su notificaci\u00f3n.Bolet\u00edn Oficial del Estado;2023. Available from:www.boe.es\/eli\/es\/rd\/2023\/10\/10\/779. Accessed June 8 2026."},{"key":"e_1_2_10_18_1","first-page":"1","article-title":"Regulation (EU) 2016\/429 of the European Parliament and of the Council of 9 March 2016 on transmissible animal diseases (\u2018Animal Health Law\u2019)","volume":"84","author":"European Parliament, Council of the European Union","year":"2016","journal-title":"Off J Eur Union."},{"key":"e_1_2_10_19_1","first-page":"1","article-title":"Commission Delegated Regulation (EU) 2020\/686 of 17 December 2019 supplementing Regulation (EU) 2016\/429 of the European Parliament and of the Council as regards the approval of germinal product establishments and the traceability and animal health requirements for movements within the Union of germinal products of certain kept terrestrial animals","volume":"174","author":"European Commission","year":"2020","journal-title":"Off J Eur Union."},{"key":"e_1_2_10_20_1","first-page":"1","article-title":"Commission Delegated Regulation (EU) 2020\/692 of 30 January 2020 supplementing Regulation (EU) 2016\/429 of the European Parliament and of the Council as regards rules for entry into the Union, and the movement and handling after entry of consignments of certain animals, germinal products and products of animal origin","volume":"174","author":"European Commission","year":"2020","journal-title":"Off J Eur Union."},{"key":"e_1_2_10_21_1","first-page":"140","article-title":"Commission Delegated Regulation (EU) 2020\/688 of 17 December 2019 supplementing Regulation (EU) 2016\/429 of the European Parliament and of the Council, as regards animal health requirements for movements within the Union of terrestrial animals and hatching eggs","volume":"174","author":"European Commission","year":"2020","journal-title":"Off J Eur Union"},{"key":"e_1_2_10_22_1","unstructured":"Anon.Daemon Quest by Deloitte: Estudio del Impacto del Sector Ecuestre en Espa\u00f1a [Study on the impact of the Equestrian Sector in Spain].Real Federacion Hipica Espa\u00f1ola (RFHE);2013. Available from:http:\/\/www.rfhe.com\/estudio\u2010global\u2010de\u2010impacto\u2010econo\/. Accessed January 20 2014."},{"key":"e_1_2_10_23_1","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1111\/eve.12672","article-title":"Equine viral arteritis: a respiratory and reproductive disease of significant economic importance to the equine industry","volume":"30","author":"Balasuriya UBR","year":"2016","journal-title":"Equine Vet Educ."},{"key":"e_1_2_10_24_1","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/B978-1-4557-0891-8.00063-4","volume-title":"Equine infectious diseases","author":"Timoney PJ","year":"2014"},{"key":"e_1_2_10_25_1","doi-asserted-by":"crossref","first-page":"946","DOI":"10.2460\/javma.2001.219.946","article-title":"Seroprevalence of antibodies against equine arteritis virus in horses residing in the United States and imported horses","volume":"219","author":"Hullinger PJ","year":"2001","journal-title":"J Am Vet Med Assoc."},{"key":"e_1_2_10_26_1","unstructured":"World Organisation for Animal Health.Codes and manuals.2025. Available from:https:\/\/www.woah.org\/en\/what\u2010we\u2010do\/standards\/codes\u2010and\u2010manuals\/. Accessed June 23 2025."},{"key":"e_1_2_10_27_1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.vetmic.2013.06.015","article-title":"Equine arteritis virus","volume":"167","author":"Balasuriya UBR","year":"2013","journal-title":"Vet Microbiol."},{"key":"e_1_2_10_28_1","first-page":"29","article-title":"Equine viral arteritis: a standard procedure for the virus neutralization test and comparison of results of a proficiency test performed at five laboratories","volume":"89","author":"Senne D","year":"1985","journal-title":"Proc U S Animal Health Assoc."},{"key":"e_1_2_10_29_1","doi-asserted-by":"crossref","first-page":"4117","DOI":"10.1016\/j.vaccine.2004.02.052","article-title":"Evidence that use of an inactivated equine herpesvirus vaccine induces serum cytotoxicity affecting the equine arteritis virus neutralisation test","volume":"22","author":"Newton J","year":"2004","journal-title":"Vaccine."},{"key":"e_1_2_10_30_1","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1292\/jvms.60.1043","article-title":"Enzyme\u2010linked immunosorbent assay for serological survey of equine arteritis virus in racehorses","volume":"60","author":"Kondo T","year":"1998","journal-title":"J Vet Med Sci."},{"key":"e_1_2_10_31_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0166-0934(00)00231-7","article-title":"Development and evaluation of ELISA procedures to detect antibodies against the major envelope protein (GL) of equine arteritis virus","volume":"90","author":"Nugent J","year":"2000","journal-title":"J Virol Methods."},{"key":"e_1_2_10_32_1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1294\/jes.29.111","article-title":"Evaluation of two enzyme\u2010linked immunosorbent assays for the detection of antibodies against equine arteritis virus","volume":"29","author":"Bannai H","year":"2018","journal-title":"J Equine Sci."},{"key":"e_1_2_10_33_1","first-page":"38","article-title":"Detection of antibodies to equine arteritis virus by a monoclonal antibody\u2010based blocking ELISA","volume":"64","author":"Cho HJ","year":"2000","journal-title":"Can J Vet Res."},{"key":"e_1_2_10_34_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1136\/vr.103362","article-title":"Further evaluation and validation of a commercially available competitive ELISA (cELISA) for the detection of antibodies specific to equine arteritis virus (EAV)","volume":"178","author":"Pfahl K","year":"2016","journal-title":"Vet Rec."},{"key":"e_1_2_10_35_1","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1136\/vr.164.14.437-a","article-title":"Testing for antibodies to equine arteritis virus","volume":"164","author":"Legrand L","year":"2009","journal-title":"Vet Rec."},{"key":"e_1_2_10_36_1","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1111\/evj.12500","article-title":"Seroprevalence and factors associated with seropositivity to equine arteritis virus in Spanish Purebred horses in Spain","volume":"48","author":"Cruz F","year":"2016","journal-title":"Equine Vet J."},{"key":"e_1_2_10_37_1","first-page":"20","article-title":"Estudio preliminar de la seroprevalencia de arteritis viral equina en caballos pura raza espa\u00f1ola de la comunidad valenciana","volume":"97","author":"Gimeno Suarep S","year":"2011","journal-title":"Actualidad Vet."},{"key":"e_1_2_10_38_1","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.rvsc.2017.01.022","article-title":"Equine viral arteritis in breeding and sport horses in central Spain","volume":"115","author":"Cruz\u2010Lopez F","year":"2017","journal-title":"Res Vet Sci."},{"key":"e_1_2_10_39_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.rvsc.2025.105701","article-title":"Equine viral arteritis: aeroprevalence patterns and risk factors in equids from western Europe","volume":"192","author":"Franco JJ","year":"2025","journal-title":"Res Vet Sci."},{"key":"e_1_2_10_40_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2307\/2529310","article-title":"The measurement of observer agreement for categorical data","volume":"33","author":"Landis JR","year":"1977","journal-title":"Biometrics."},{"key":"e_1_2_10_41_1","unstructured":"Ministerio de Agricultura Pesca y Alimentaci\u00f3n.Registro: Sistema Integral de Trazabilidad Animal (SITRAN).2024. Available from:https:\/\/www.mapa.gob.es\/eu\/ganaderia\/temas\/trazabilidad\u2010animal\/sitran\/default.aspx. Accessed March 1 2024."},{"key":"e_1_2_10_42_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0166-0934(95)00020-U","article-title":"Development and evaluation of an ELISA using recombinant fusion protein to detect the presence of host antibody to equine arteritis virus","volume":"54","author":"Chirnside ED","year":"1995","journal-title":"J Virol Methods."},{"key":"e_1_2_10_43_1","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/0378-1135(89)90041-2","article-title":"The effects of vaccination with tissue culture\u2010derived viral vaccines on detection of antibodies to equine arteritis virus by enzyme\u2010linked immunosorbent assay (ELISA)","volume":"20","author":"Cook RF","year":"1989","journal-title":"Vet Microbiol."},{"key":"e_1_2_10_44_1","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1177\/1040638713508401","article-title":"Validation of an improved competitive enzyme\u2010linked immunosorbent assay to detect Equine arteritis virus antibody","volume":"25","author":"Chung C","year":"2013","journal-title":"J Vet Diag Investig."},{"key":"e_1_2_10_45_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.prevetmed.2023.106096","article-title":"Short communication: retrospective analysis of obligatory testing results for Equine virus arteritis reveals a decrease of its seroprevalence in stallions used for artificial insemination","volume":"223","author":"Kaps M","year":"2024","journal-title":"Prevent Vet Med."},{"key":"e_1_2_10_46_1","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/S0737-0806(07)80023-2","article-title":"Equine viral arteritis in perspective in relation to international trade","volume":"13","author":"Timoney PJ","year":"1993","journal-title":"J Equine Vet Sci."},{"key":"e_1_2_10_47_1","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/S0749-0739(17)30094-9","article-title":"Factors influencing the international spread of equine diseases","volume":"16","author":"Timoney PJ","year":"2000","journal-title":"Vet Clin N Am Equine Pract."},{"key":"e_1_2_10_48_1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.theriogenology.2008.04.020","article-title":"Equine viral arteritis: current status and prevention","volume":"70","author":"Holyoak GR","year":"2008","journal-title":"Theriogenology"},{"key":"e_1_2_10_49_1","first-page":"263","article-title":"Studies of an epizootic of equine viral arteritis in racehorses","volume":"2","author":"McCollum WH","year":"1978","journal-title":"J Equine Med Surg."},{"key":"e_1_2_10_50_1","doi-asserted-by":"crossref","first-page":"2142","DOI":"10.3390\/v13112142","article-title":"Equine arteritis virus (EAV) Outbreak in a show stallion population","volume":"13","author":"Otzdorff C","year":"2021","journal-title":"Viruses."},{"key":"e_1_2_10_51_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1080\/01652176.1996.9694625","article-title":"Equine arteritis virus: a review of clinical features and management aspects","volume":"18","author":"Glaser AL","year":"1996","journal-title":"Vet Q."},{"key":"e_1_2_10_52_1","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.vetmic.2010.09.008","article-title":"Epizotiology and phylogeny of equine arteritis virus in Hucul horses","volume":"148","author":"Rola J","year":"2011","journal-title":"Vet Microbiol."}],"container-title":["Veterinary Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vetr.71223","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vetr.71223","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vetr.71223","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:37:34Z","timestamp":1790818654000},"score":1,"resource":{"primary":{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/10.1002\/vetr.71223"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":51,"alternative-id":["10.1002\/vetr.71223"],"URL":"https:\/\/doi.org\/10.1002\/vetr.71223","archive":["Portico"],"relation":{},"ISSN":["0042-4900","2042-7670"],"issn-type":[{"value":"0042-4900","type":"print"},{"value":"2042-7670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"article-number":"vetr.71223"}}