{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T21:21:10Z","timestamp":1776460870930,"version":"3.51.2"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,1,7]],"date-time":"2023-01-07T00:00:00Z","timestamp":1673049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2023,3]]},"abstract":"Abstract<\/jats:title>\n \n Backgrounds<\/jats:title>\n Transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2) type I receptor (TGF\u03b2RI) and type II receptor (TGF\u03b2RII) are the members of the TGF\u03b2 superfamily, which are potent regulators of cell proliferation and differentiation in many organ systems, and they play key roles in multiple aspects of follicle development.<\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n We aimed to explore the characterization, expression analysis of\n TGF\u03b2RI<\/jats:italic>\n and\n TGF\u03b2RII<\/jats:italic>\n genes, and the association with litter size in Tibetan sheep.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n In this study, we cloned the complete coding sequences of\n TGF\u03b2RI<\/jats:italic>\n and\n TGF\u03b2RII<\/jats:italic>\n genes in Tibetan sheep and analyzed their genomic structures.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The results showed that percentages of sequences homology of the two proteins in Tibetan sheep were the most similar to\n Ovis aries<\/jats:italic>\n (100%), followed by\n Bos mutus<\/jats:italic>\n (99%). The RT\u2010qPCR showed that two genes were expressed widely in the different tissues of Tibetan sheep. The\n TGF\u03b2RI<\/jats:italic>\n expression was the highest in the lung (\n p<\/jats:italic>\n < 0.05), followed by the spleen and ovary (\n p<\/jats:italic>\n < 0.05). The\n TGF\u03b2RII<\/jats:italic>\n expression was significantly higher in uterus than that in lung and ovary (\n p<\/jats:italic>\n < 0.05). In addition, the\n \u03c7<\/jats:italic>\n 2<\/jats:sup>\n test indicated that all ewes in the population were in Hardy\u2013Weinberg equilibrium, and the population was in medium or low polymorphic information content status. We also found four Single Nucleotide Polymorphism (SNPs), g.9414A > G, g.28881A > G, g.28809T > C, g.10429G > A in sheep\n TGF\u03b2RI<\/jats:italic>\n gene and g.63940C > T, g.63976C > T, g.64538C > T, g.64504T > A in\n TGF\u03b2RII<\/jats:italic>\n gene. Three genotypes, except for g.64504T > A, and three haplotypes were identified in each gene. linkage disequilibrium analysis indicated that there was strong linkage disequilibrium in each gene. The association analysis showed that the four SNPs of\n TGF\u03b2RI<\/jats:italic>\n were associated with litter size (\n p<\/jats:italic>\n < 0.05), and g.63940C > T of\n TGF\u03b2R<\/jats:italic>\n II was confirmed to be associated with litter size (\n p<\/jats:italic>\n < 0.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Based on these preliminary results, we can assume that TGF\u03b2 receptors (TGF\u03b2RI and TGF\u03b2RII) may play an important role in sheep reproduction.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/vms3.1013","type":"journal-article","created":{"date-parts":[[2023,1,7]],"date-time":"2023-01-07T03:40:49Z","timestamp":1673062849000},"page":"934-944","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular characterization and expression of\n TGF\u03b2RI<\/i>\n and\n TGF\u03b2RII<\/i>\n and its association with litter size in Tibetan sheep"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5561-0290","authenticated-orcid":false,"given":"Junxia","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Agriculture and Animal Husbandry Key Laboratory of Livestock and Poultry Genetics and Breeding on the Qinghai\u2010Tibet Plateau Ministry of Agriculture and Rural Affairs Plateau Livestock Genetic Resources Protection and Innovative Utilization Key Laboratory of Qinghai Province Qinghai University Xining P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingming","family":"Li","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry Key Laboratory of Livestock and Poultry Genetics and Breeding on the Qinghai\u2010Tibet Plateau Ministry of Agriculture and Rural Affairs Plateau Livestock Genetic Resources Protection and Innovative Utilization Key Laboratory of Qinghai Province Qinghai University Xining P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruizhe","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry Key Laboratory of Livestock and Poultry Genetics and Breeding on the Qinghai\u2010Tibet Plateau Ministry of Agriculture and Rural Affairs Plateau Livestock Genetic Resources Protection and Innovative Utilization Key Laboratory of Qinghai Province Qinghai University Xining P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"He","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry Key Laboratory of Livestock and Poultry Genetics and Breeding on the Qinghai\u2010Tibet Plateau Ministry of Agriculture and Rural Affairs Plateau Livestock Genetic Resources Protection and Innovative Utilization Key Laboratory of Qinghai Province Qinghai University Xining P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaocheng","family":"Wen","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry Key Laboratory of Livestock and Poultry Genetics and Breeding on the Qinghai\u2010Tibet Plateau Ministry of Agriculture and Rural Affairs Plateau Livestock Genetic Resources Protection and Innovative Utilization Key Laboratory of Qinghai Province Qinghai University Xining P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueping","family":"Han","sequence":"additional","affiliation":[{"name":"Technology Extension Service of Animal Husbandry of Qinghai Xining China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zenghai","family":"Luo","sequence":"additional","affiliation":[{"name":"Technology Extension Service of Animal Husbandry of Qinghai Xining China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,7]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1359\u20106101(96)00042\u20101"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1071809"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/7.11.1047"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0303\u20107207(99)00185\u20109"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.13.15.2105"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0898\u20106568(96)00122\u20102"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349\u20107006.2009.01441.x"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40659\u2010020\u201000290\u20107"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477\u20107827\u20102\u201078"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0378\u20104320(03)00089\u20107"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.01074"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585\u2010021\u201010077\u2010z"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2009.09.007"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/2049\u20101891\u20105\u201052"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441\u2010005\u20100008\u20100"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2013.1860"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472\u20106882\u201014\u2010148"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403\u2010019\u201001960\u20107"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/6.6.498"},{"key":"e_1_2_11_21_1","first-page":"193","volume-title":"Embryonic stem cells: The hormonal regulation of pluripotency and embryogenesis","author":"Ovchinnikov D. 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