{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:15:36Z","timestamp":1772118936020,"version":"3.50.1"},"reference-count":62,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T00:00:00Z","timestamp":1671408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2023,1]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Cryptosporidium<\/jats:italic>\n spp. are opportunistic intestinal protozoans with global distribution and are of great importance as zoonotic protozoans are common to humans and domestic animals, including cattle and calves. Identification and detection of parasite species using precise methods including molecular methods can be an effective step in treating and controlling parasites.\n <\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n This study aimed to investigate the prevalence of\n Cryptosporidium<\/jats:italic>\n among breeding calves of Khorramabad city, Lorestan province, Western Iran, using PCR.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The faecal samples were taken from 181 healthy and diarrhoeal calves and after the Ziehl Neelsen Acid\u2010fast staining and microscopic evaluation, the genomic DNA was extracted for molecular evaluations. To detect\n Cryptosporidium<\/jats:italic>\n species, specific primers targeting the SAM\u20101 gene of\n Cryptosporidium<\/jats:italic>\n and a commercial master mix were used for PCR.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Out of 181 faecal samples of breeding calves in Khorramabad city, 9 samples (5%) were positive for\n Cryptosporidium<\/jats:italic>\n spp. using the PCR method. Statistical analysis of the data showed that there was no significant statistical relationship between\n Cryptosporidium<\/jats:italic>\n infection of the calves and variables of age, breed, type of water consumption, clinical signs of diarrhoea, and sampling location, while parasite infection had a significant relationship with calf gender so that all\n Cryptosporidium<\/jats:italic>\n positive samples were from male calves (\n p<\/jats:italic>\n \u2264 0.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Considering the presence of\n Cryptosporidium<\/jats:italic>\n infection, the region's traditional grazing system, and the close relationship between livestock and humans, there is a possibility of human infection in the region. So preventive measures such as periodic animal testing with sensitive and accurate diagnostic techniques including PCR, pharmacological treatment of livestock, water hygiene and the use of industrial grazing instead of traditional grazing to improve the hygiene of food consumed by livestock are recommended.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/vms3.1024","type":"journal-article","created":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T10:08:48Z","timestamp":1671444528000},"page":"363-371","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular evaluation of\n Cryptosporidium<\/i>\n spp. among breeding calves of Lorestan province Western Iran"],"prefix":"10.1002","volume":"9","author":[{"given":"Razieh","family":"Talebi","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology and Mycology School of Medicine Lorestan University of Medical Sciences Khorramabad 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University of Medical Sciences Khorramabad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farzad","family":"Ebrahimzadeh","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Epidemiology, School of Health and Nutrition Lorestan University of Medical Sciences Khorramabad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamad","family":"Kazempour","sequence":"additional","affiliation":[{"name":"Department of Chemistry Tabriz Branch Islamic Azad University Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3791-9312","authenticated-orcid":false,"given":"Nozhat","family":"Zebardast","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center School of Medicine Guilan University of Medical Sciences Rasht 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