{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T18:24:03Z","timestamp":1780597443075,"version":"3.54.1"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,5,23]],"date-time":"2018-05-23T00:00:00Z","timestamp":1527033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"HIS MAJESTY's Sultan Qaboos Bin Said for strategic research fund","award":["SR\/ANVS\/14\/01"],"award-info":[{"award-number":["SR\/ANVS\/14\/01"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Brucellosis, one of the most common zoonotic diseases and has significant public health and economic importance worldwide. Few studies and reports have been performed to estimate the true prevalence of animal brucellosis in the Sultanate of Oman; however, no incidence of the disease was previously reported in Al Jabal Al Akhdar. The purpose of this study was to investigate the prevalence of brucellosis in goats in eight villages in Al Jebal Al Akhdar, Sultanate of Oman, namely: Al Aqaieb, Al Helailat, Al Ghilayil, Hail Al Hedap, Da'an Al Hamra, Shnoot, Al Qasha'e and Al Sarah<jats:bold>,\u00a0<\/jats:bold>Al Jabal Al Akhdar in the Sultanate of Oman. In this study we used different diagnostic serological tests, namely, <jats:styled-content style=\"fixed-case\">RBT<\/jats:styled-content>, I\u2010<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CFT<\/jats:styled-content> to study the prevalence of Brucella infection in goats in Al Jabal Al Akhdar. Statistical analysis using Kappa statistics was used to compare the performance of the serological tests. Biochemical tests and species\u2010specific Multiplex <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> were used to identify the <jats:italic>brucella<\/jats:italic> species involved in the infection. A structured questionnaire and Chi\u2010square (x<jats:sup>2<\/jats:sup>) statistical analysis was used to identify related brucellosis risk factors. This study is the first to reveal brucellosis infection in goats in eight villages in Al Jebal Al Akhdar, Sultanate of Oman, namely: Al Aqaieb, Al Helailat, Al Ghilayil, Hail Al Hedap, Da'an Al Hamra, Shnoot, Al Qasha'e and Al Sarah<jats:bold>,<\/jats:bold> with an overall seroprevalence of 11.1%. The study also compared the performance of three different serological tests, namely, <jats:styled-content style=\"fixed-case\">RBT<\/jats:styled-content>, I\u2010<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CFT<\/jats:styled-content>. Statistical analysis using Kappa statistics showed that the degree of agreement was best seen between <jats:styled-content style=\"fixed-case\">RBT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CFT<\/jats:styled-content> (96%), followed by <jats:styled-content style=\"fixed-case\">RBT<\/jats:styled-content>, I\u2010 <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> (91.4%) and <jats:styled-content style=\"fixed-case\">CFT<\/jats:styled-content> and I\u2010 <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> (89.2%). Biochemical tests and species\u2010specific Multiplex <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> showed the typical profile for<jats:italic>\u00a0B. melitensis<\/jats:italic>. A structured questionnaire and Chi\u2010square (x<jats:sup>2<\/jats:sup>) statistical analysis indicated that the presence of abortion is the major risk factor for the prevalence of brucellosis, whereas age and sex were not significant factors in the tested animals. Besides, poor knowledge about brucellosis, consumption of unpasteurized milk or milk products, free trade of animals and the introduction of new animal breeds to herds were all contributing risk factors to the prevalence of brucellosis. The prevalence of human brucellosis obtained verbally from pastoralists gave an insight that brucellosis could pose a public health hazard, especially in those high\u2010risk groups, mainly the pastoralists in the study area. Because of their constant and increasing interaction with their animals, pastoralists could be at a high risk of occupational infection.<\/jats:p>","DOI":"10.1002\/vms3.103","type":"journal-article","created":{"date-parts":[[2018,5,23]],"date-time":"2018-05-23T07:10:06Z","timestamp":1527059406000},"page":"190-205","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Serological, cultural and molecular evidence of <i>Brucella melitensis<\/i> infection in goats in Al Jabal Al Akhdar, Sultanate of Oman"],"prefix":"10.1002","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0246-826X","authenticated-orcid":false,"given":"Yasmin","family":"ElTahir","sequence":"first","affiliation":[{"name":"College of Agricultural &amp; Marine Sciences Department of Animal &amp; Veterinary Sciences Sultan Qaboos University Alkhod Sultanate of Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Al Ghalya","family":"Al Toobi","sequence":"additional","affiliation":[{"name":"College of Agricultural &amp; Marine Sciences Department of Animal &amp; Veterinary Sciences Sultan Qaboos University Alkhod Sultanate of Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waleed","family":"Al\u2010Marzooqi","sequence":"additional","affiliation":[{"name":"College of Agricultural &amp; Marine Sciences Department of Animal &amp; Veterinary Sciences Sultan Qaboos University Alkhod Sultanate of Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osman","family":"Mahgoub","sequence":"additional","affiliation":[{"name":"College of Agricultural &amp; Marine Sciences Department of Animal &amp; Veterinary Sciences Sultan Qaboos University Alkhod Sultanate of Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryne","family":"Jay","sequence":"additional","affiliation":[{"name":"EU\/OIE\/FAO &amp; National Reference Laboratory for Brucellosis Animal Health Laboratory Paris\u2010Est University\/Anses Maisons\u2010Alfort France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yannick","family":"Corde","sequence":"additional","affiliation":[{"name":"EU\/OIE\/FAO &amp; National Reference Laboratory for Brucellosis Animal Health Laboratory Paris\u2010Est University\/Anses Maisons\u2010Alfort France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadi","family":"Al Lawati","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture &amp; Fisheries Directorate General of Animal wealth Muscat Sultanate of Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shekar","family":"Bose","sequence":"additional","affiliation":[{"name":"Department of Natural Resources Economics Sultan Qaboos University Alkhod Sultanate of Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abeer","family":"Al Hamrashdi","sequence":"additional","affiliation":[{"name":"College of Agricultural &amp; Marine Sciences Department of Animal &amp; Veterinary Sciences Sultan Qaboos University Alkhod Sultanate of Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaadhia","family":"Al Kharousi","sequence":"additional","affiliation":[{"name":"College of Agricultural &amp; Marine Sciences Department of Animal &amp; Veterinary Sciences Sultan Qaboos University Alkhod Sultanate of Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasseb","family":"Al\u2010Saqri","sequence":"additional","affiliation":[{"name":"College of Agricultural &amp; Marine Sciences Department of Animal &amp; Veterinary Sciences Sultan Qaboos University Alkhod Sultanate of Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudaina","family":"Al Busaidi","sequence":"additional","affiliation":[{"name":"College of Agricultural &amp; Marine Sciences Department of Animal &amp; Veterinary Sciences Sultan Qaboos University Alkhod Sultanate of Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugene H.","family":"Johnson","sequence":"additional","affiliation":[{"name":"College of Agricultural &amp; Marine Sciences Department of Animal &amp; Veterinary Sciences Sultan Qaboos University Alkhod Sultanate of Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,5,23]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"5","article-title":"Brucella biovars isolated from domestic livestock in Dhofar Province in the Sultanate of Oman","volume":"6","author":"Adam A.M.","year":"2013","journal-title":"Veterinary Research"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.5110"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268803001857"},{"key":"e_1_2_11_5_1","unstructured":"Al\u2010RawahiA. 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