{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:52:39Z","timestamp":1791499959557,"version":"4.3.4"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,30]],"date-time":"2022-12-30T00:00:00Z","timestamp":1672358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U20A20361"],"award-info":[{"award-number":["U20A20361"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82171819"],"award-info":[{"award-number":["82171819"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2018YFC1603705"],"award-info":[{"award-number":["2018YFC1603705"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Brucellosis, caused by <jats:italic>Brucella spp<\/jats:italic>., is a major zoonotic public health threat. Although several <jats:italic>Brucella<\/jats:italic> vaccines have been demonstrated for use in animals, <jats:italic>Brucella spp<\/jats:italic>. can cause human infection and to date, there are no human\u2010use vaccines licensed by any agency. Recently, methods in vaccine informatics have made major breakthroughs in peptide\u2010based epitopes, opening up a new avenue of vaccine development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The purpose of this article was to identify potential antigenic peptides in <jats:italic>Brucella<\/jats:italic> by proteome and peptidome analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mouse infection models were first established by injection with <jats:italic>Brucella<\/jats:italic> and spleen protein profiles were then analysed. Subsequently, the major histocompatibility complex class I or II (major histocompatibility complex [MHC]\u2010I\/II)\u2010binding peptides in blood samples were collected by immunoprecipitation and peptides derived from <jats:italic>Brucella<\/jats:italic> proteins were identified through liquid chromatography\u2013mass spectrometry (LC\u2010MS\/MS). These peptides were then evaluated in a variety of ways, such as in terms of conservation in <jats:italic>Brucella<\/jats:italic> and synchronicity in predicted peptides (similarity and coverage), which allowed us to more effectively measure their antigenic potential.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression of the inflammatory cytokines IL1B and IFN\u2010\u03b3 was significantly altered in the spleen of infected mice and some <jats:italic>Brucella<\/jats:italic> proteins, such as Muri, AcpP and GroES, were also detected. Meanwhile, in blood, 35 peptides were identified and most showed high conservation, highlighting their potential as antigen epitopes for vaccine development. In particular, we identified four proteins containing both MHC\u2010I\u2010 and MHC\u2010II\u2010binding peptides including AtpA, AtpD, DnaK and BAbS19_II02030. They were also compared with the predicted peptides to estimate their reliability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The peptides we screened could bind to MHC molecules. After being stimulated with antigen T epitopes, Memory T cells can stimulate T cell activation and promote immune responses. Our results indicated that the peptides we identified may be good candidate targets for the design of subunit vaccines and these results pave the way for the study of safer vaccines against <jats:italic>Brucella<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/vms3.1048","type":"journal-article","created":{"date-parts":[[2022,12,30]],"date-time":"2022-12-30T18:17:56Z","timestamp":1672424276000},"page":"523-534","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Identification of potential antigenic peptides of <i>Brucella<\/i> through proteome and peptidome"],"prefix":"10.1002","volume":"9","author":[{"given":"Meijuan","family":"Pei","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory The Third Medical Centre of Chinese PLA General Hospital The Training Site for Postgraduate of Jinzhou Medical University  Beijing China"},{"name":"State Key Laboratory of Pathogen and Biosecurity Beijing 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