{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T21:33:44Z","timestamp":1770154424983,"version":"3.49.0"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T00:00:00Z","timestamp":1675900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was to evaluate the effects of zinc chloride (ZnCl<jats:sub>2<\/jats:sub>) at 20, 50, 100, and 200\u00a0\u03bcg\/mL on the quality of seminal plasma\u2010free boar sperm stored at 17\u00b0C for 7 days and to explore the underlying mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Boar sperm were collected and incubated in non\u2010capacitation\/capacitation medium to analyze sperm quality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the non\u2010capacitated state, the addition of ZnCl<jats:sub>2<\/jats:sub> at 20 and 50\u00a0\u03bcg\/mL improved the survival rate and plasma membrane integrity of boar sperm (<jats:italic>p<\/jats:italic> &lt; 0.05). Compared to the control group, the addition of ZnCl<jats:sub>2<\/jats:sub> significantly increased total antioxidative capacity and CuZn superoxide dismutase activity, while reducing the malondialdehyde content (<jats:italic>p<\/jats:italic> &lt; 0.05). ZnCl<jats:sub>2<\/jats:sub> at 100 and 200\u00a0\u03bcg\/mL significantly decreased sperm motility, protein kinase A (PKA) substrate phosphorylation, and tyrosine phosphorylation. These proteins were mainly located on the mid\u2010pieces of the flagellum. The addition of ZnCl<jats:sub>2<\/jats:sub> at 20 and 50\u00a0\u03bcg\/mL conveyed a protective effect to boar sperm stored at 17\u00b0C. Furthermore, ZnCl<jats:sub>2<\/jats:sub> at 100 and 200\u00a0\u03bcg\/mL inhibited sperm motility via tyrosine phosphorylation, thus preventing the \u2018capacitation\u2010like\u2019 state. In the capacitated state, there was no change in PKA substrate phosphorylation and tyrosine phosphorylation of the mid\u2010pieces of the flagellum compared to the control groups, indicating that the addition of Zn<jats:sup>2+<\/jats:sup> did not negatively affect capacitation of preserved sperm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>ZnCl<jats:sub>2<\/jats:sub> showed protective capacity to the preservation extender used for boar sperm during the process of 17\u00b0C storage, and the optimal concentration of ZnCl<jats:sub>2<\/jats:sub> for the preservation extender was 100\u00a0\u03bcg\/mL.<\/jats:p><\/jats:sec>","DOI":"10.1002\/vms3.1094","type":"journal-article","created":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T13:01:10Z","timestamp":1675947670000},"page":"1217-1225","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects of zinc chloride on boar sperm quality during liquid storage at 17\u00b0C"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2400-4500","authenticated-orcid":false,"given":"Qimeng","family":"Hu","sequence":"first","affiliation":[{"name":"Hebei Key Laboratory of Animal Diversity College of Life Sciences, Langfang Normal University  Langfang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,2,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13547"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ucl.2020.09.009"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2014.02.013"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.5534\/wjmh.2014.32.1.1"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gap099"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00269"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2016.0173"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00612"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.23128"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1515\/pjvs\u20102015\u20100095"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00270a032"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/der132"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1071\/rd9960581"},{"key":"e_1_2_10_15_1","first-page":"137","article-title":"Zona pellucida binding assay\u2010a method for evaluation of canine spermatozoa","volume":"57","author":"Holst B. 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