{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T23:04:21Z","timestamp":1793315061309,"version":"4.5.0"},"reference-count":61,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T00:00:00Z","timestamp":1684281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>This study investigates the effects of cryopreservation and supplementation of Azeri water buffalo's semen with proline (Lp) and fulvic acid (FA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Therefore, this study aimed to assess motility parameters, sperm viability, oxidative stress parameters, and DNA damage to detect the optimum concentrations of Lp and FA for buffalo semen cryopreservation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Thirty semen samples of three buffalo bulls were diluted in Tris\u2010egg yolk extender and divided into 12 equal groups including control (C), Lp\u201010, Lp\u201020, Lp\u201040, Lp\u201060, Lp\u201080 (containing 10, 20, 40, 60, 80\u00a0mM L\u2010proline, respectively), FA\u20100.2, FA\u20100.5, FA\u20100.8, FA\u20101.1, FA\u20101.4 and FA\u20101.7 (containing 0.2%, 0.5%, 0.8%, 1.1%, 1.4% and 1.7% fulvic acid, respectively).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The velocity parameters, TM and PM were improved by FA\u20101.7, FA\u20101.4, Lp\u201040 and Lp\u201060 groups compared to the C group but no significant difference was found regarding the amplitude of lateral head displacement and straightness compared to the control groups. The percentage of sperm viability and PMF were increased by FA\u20101.7, FA\u20101.4, FA\u20101.1, Lp\u201040 and Lp\u201060 groups compared to C group, while in terms of sperm DNA damage FA\u20101.7, FA\u20101.4, FA\u20101.1, Lp\u201010, Lp\u201020, Lp\u201040 and Lp\u201060 groups showed better results compared to C group. The results also showed that FA\u20101.7, FA\u20101.4, FA\u20101.1, Lp\u201020, Lp\u201040 and Lp\u201060 groups could improve TAC, SOD, GSH and decrease MDA levels. Also, FA\u20101.7, FA\u20101.4, Lp\u201020 and Lp\u201040 groups could improve GPx levels but just FA\u20101.7, and Lp\u201040 groups could improve CAT levels compared to C group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Thus, it can be concluded that L\u2010proline and fulvic acid supplementations can improve the quality parameters of post\u2010thawed buffalo bull semen.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.1158","type":"journal-article","created":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T19:53:36Z","timestamp":1684353216000},"page":"1791-1802","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":71,"title":["The influence of L\u2010proline and fulvic acid on oxidative stress and semen quality of buffalo bull semen following cryopreservation"],"prefix":"10.1002","volume":"9","author":[{"given":"Negin","family":"Ramazani","sequence":"first","affiliation":[{"name":"Researcher in Biology and Animal Reproduction  Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farid","family":"Mahd Gharebagh","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine Urmia University  Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1591-2198","authenticated-orcid":false,"given":"Ali","family":"Soleimanzadeh","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine Urmia University  Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Halil Ozancan","family":"Arslan","sequence":"additional","affiliation":[{"name":"Republic of Turkey Ministry of Agriculture and Foresty International Center for Livestock Research and Training  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esin","family":"Keles","sequence":"additional","affiliation":[{"name":"Republic of Turkey Ministry of Agriculture and Foresty International Center for Livestock Research and Training  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1096-0972","authenticated-orcid":false,"given":"Desislava Georgieva","family":"Gradinarska\u2010Yanakieva","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biotechnologies and Cryobiology of Gametes Institute of Biology and Immunology of Reproduction \u2018Acad. Kiril Bratanov\u2019 at Bulgarian Academy of Sciences  Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damla","family":"Arslan\u2010Acar\u00f6z","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Biochemistry Afyon Kocatepe University  Afyonkarahisar Turkey"},{"name":"ACR Bio Food and Biochemistry Research and Development  Afyonkarahisar Turkey"},{"name":"Faculty of Veterinary Medicine Kyrgyz\u2010Turkish Manas University  Bishkek Kyrgyzstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2886-9747","authenticated-orcid":false,"given":"Mahdi","family":"Zhandi","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Department of Animal Science College of Agriculture and Natural Resources University of Tehran  Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alper","family":"Baran","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Reproduction and Artificial Insemination Clinical Sciences, Istanbul University\u2010Cerrahpasa  Avcilar\u2010Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esmail","family":"Ayen","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine Urmia University  Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dursun Ali","family":"Din\u00e7","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Obstetrics and Gynecology University of Selcuk  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,5,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.21608\/jappmu.2019.43014"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.05.010"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.10.024"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2019.11.044"},{"key":"e_1_2_11_6_1","first-page":"1499","article-title":"Genetic diversity in buffalo population of Guilan using microsatellite markers","volume":"7","author":"Aminafshar M.","year":"2008","journal-title":"Journal of Animal and Veterinary Advances"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbio.2012.10.001"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.5194\/aab-55-415-2012"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2013.10.013"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/686137"},{"issue":"6","key":"e_1_2_11_11_1","first-page":"1260","article-title":"The effect of cysteine on post\u2010thawed buffalo bull (Bubalus bubalis) sperm parameters","volume":"5","author":"Beheshti R.","year":"2011","journal-title":"Advances in Environmental Biology"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12279"},{"key":"e_1_2_11_13_1","first-page":"1","article-title":"Buffalo population and strategies in the world","volume":"67","author":"Borghese A.","year":"2005","journal-title":"Buffalo Production and Research"},{"key":"e_1_2_11_14_1","first-page":"1836","article-title":"Effects of dietary FA on the growth performance, serum biochemical indices, routine blood parameter and immunity of growing swine","volume":"25","author":"Chang Q. 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