{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:15:36Z","timestamp":1772118936327,"version":"3.50.1"},"reference-count":66,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T00:00:00Z","timestamp":1692576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007298","name":"Urmia University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007298","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Chlamydiaceae are a group of gram\u2010negative intracellular bacteria which can infect a wide variety of hosts. Some chlamydial agents are capable of crossing the host barrier and though they are potentially a risk to very different species. They also pose a zoonotic risk for human and different chlamydial agents are linked to several medical maladies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>In this study, the presence of chlamydial agents in different commercial poultry flocks in Iran was investigated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Swab and tissue samples were collected from 435 birds in 24 different commercial poultry flocks. These samples were examined using a Chlamydiaceae\u2010specific real\u2010time PCR assay targeting 23S rRNA gene. Positive samples then were subjected to intergenic spacer rRNA (IGS) gene and major outer membrane protein gene (\n                      <jats:italic>omp<\/jats:italic>\n                      A) PCRs. Finally, positive PCR products were sequenced and analysed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Only one flock of commercial turkey became positive. Partial DNA sequencing of IGS gene revealed that all positive samples from the infected flock were\n                      <jats:italic>Chlamydia pneumoniae<\/jats:italic>\n                      and were identical to previously studied isolates from koala (LPCoLN) and frog (DC9). Further investigations showed slight dissimilarity in\n                      <jats:italic>omp<\/jats:italic>\n                      A gene of\n                      <jats:italic>C. pneumoniae<\/jats:italic>\n                      from different hosts. The detected turkey isolates were located in a different clade of phylogenetic tree, close to Western barred bandicoot and koala isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>C. pneumoniae<\/jats:italic>\n                      has passed the cross\u2010species barrier in the past and therefor it could potentially be zoonotic. To the best of authors\u2019 knowledge, this is the first report of\n                      <jats:italic>C. pneumoniae<\/jats:italic>\n                      infection in commercial turkey.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.1244","type":"journal-article","created":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T23:09:22Z","timestamp":1692659362000},"page":"2168-2175","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A molecular survey of\n                    <i>Chlamydia<\/i>\n                    spp. infection in commercial poultry and detection of\n                    <i>Chlamydia pneumoniae<\/i>\n                    in a commercial turkey flock in Iran"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5130-3288","authenticated-orcid":false,"given":"Seyed Mohammad Mahdi","family":"Hashemian","sequence":"first","affiliation":[{"name":"Department of Poultry Health and Diseases, Faculty of Veterinary Medicine University of Urmia  Urmia Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6498-2942","authenticated-orcid":false,"given":"Seyed Ahmad","family":"Madani","sequence":"additional","affiliation":[{"name":"Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine University of Tehran  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8656-0014","authenticated-orcid":false,"given":"Manoochehr","family":"Allymehr","sequence":"additional","affiliation":[{"name":"Department of Poultry Health and Diseases, Faculty of Veterinary Medicine University of Urmia  Urmia Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6476-0488","authenticated-orcid":false,"given":"Alireza","family":"Talebi","sequence":"additional","affiliation":[{"name":"Department of Poultry Health and Diseases, Faculty of Veterinary Medicine University of Urmia  Urmia Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,8,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2005.03.002"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a112516"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004300050071"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u20100691.2008.02130.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1078\/0723\u20102020\u201000086"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1758\u20102229.2010.00174.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)89423-4"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287\u2010137\u201011\u20102525"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00698\u201010"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.5539\/ijb.v3n4p79"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2005.09.003"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2014.993593"},{"key":"e_1_2_10_14_1","first-page":"59","article-title":"Seroprevalence of turkeys chlamydiosis in Khuzestan province","volume":"63","author":"Ghorbanpoor M.","year":"2007","journal-title":"Journal of Veterinary Research"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.18502\/ijm.v12i4.3937"},{"issue":"3","key":"e_1_2_10_16_1","first-page":"220","article-title":"Immunization against trachoma","volume":"65","author":"Grayston J. 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