{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:56:41Z","timestamp":1786978601662,"version":"build-2736575974"},"reference-count":73,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,9]],"date-time":"2023-11-09T00:00:00Z","timestamp":1699488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The European bison (\n                      <jats:italic>Bison bonasus<\/jats:italic>\n                      ), a symbol of Polish nature, is a protected species that requires active health monitoring. However, conservation efforts are made difficult by the zoonotic diseases such as brucellosis and tuberculosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      The aim of this study was to screen the Polish European bison population for exposure to the\n                      <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                      complex (MTC) and\n                      <jats:italic>Brucella<\/jats:italic>\n                      spp.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 323 free\u2010living and captive European bison from 13 localities were tested serologically for antibodies against the\n                      <jats:italic>M. bovis<\/jats:italic>\n                      P22 multi\u2010protein complex (in\u2010house ELISA) and against\n                      <jats:italic>Brucella<\/jats:italic>\n                      spp. (commercial ELISA).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Antibodies against the MTC (P22) were detected in 7% (22\/323) of the tested European bison. Anti\u2010MTC antibody positivity was not significantly different by sex, age, and captive\/free range status. Anti\u2010MTC antibodies were found in six of 13 populations sampled, always in populations with larger sample sizes including the four free\u2010living ones. Antibodies against\n                      <jats:italic>Brucella<\/jats:italic>\n                      spp. were detected in 36% (116\/323) of the tested bison. While\n                      <jats:italic>Brucella<\/jats:italic>\n                      spp. antibody prevalence was not different by sex, it was significantly different by age (lower in adults) and captive\/free\u2010living status.\n                      <jats:italic>Brucella<\/jats:italic>\n                      spp. seroprevalence decreased with sample size and seropositive bison were found in 12 of 13 sampling populations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our findings identify potential emerging threats to the European bison population and confirm the first serological response to P22 in European bison. As Poland is currently officially free of brucellosis and bovine tuberculosis, our results require careful interpretation. Further studies are needed to establish the presence of cross\u2010reactions with atypical mycobacteria in the case of MTC and other bacteria (e.g.\n                      <jats:italic>Yersinia enterocolitica<\/jats:italic>\n                      O:9) in the case of\n                      <jats:italic>Brucella<\/jats:italic>\n                      spp.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.1314","type":"journal-article","created":{"date-parts":[[2023,11,9]],"date-time":"2023-11-09T14:45:25Z","timestamp":1699541125000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antibodies against the\n                    <i>Mycobacterium tuberculosis<\/i>\n                    complex and\n                    <i>Brucella<\/i>\n                    spp. in captive and free\u2010living European bison (\n                    <i>Bison bonasus<\/i>\n                    ) in Poland"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1073-174X","authenticated-orcid":false,"given":"Anna","family":"Didkowska","sequence":"first","affiliation":[{"name":"Department of Food Hygiene and Public Health Protection Institute of Veterinary Medicine Warsaw University of Life Sciences (SGGW)  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4790-5259","authenticated-orcid":false,"given":"Elisa Ferreras","family":"Colino","sequence":"additional","affiliation":[{"name":"SaBio Instituto de Investigaci\u00f3n en Recursos Cineg\u00e9ticos IREC Consejo Superior de Investigaciones Cient\u00edficas, Universidad de Castilla \u2013 La Mancha  Ciudad Real Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6166-3954","authenticated-orcid":false,"given":"Wanda","family":"Olech","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics and Conservation Institute of Animal Sciences University of Life Sciences (SGGW)  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugguette","family":"Gloddy","sequence":"additional","affiliation":[{"name":"SaBio Instituto de Investigaci\u00f3n en Recursos Cineg\u00e9ticos IREC Consejo Superior de Investigaciones Cient\u00edficas, Universidad de Castilla \u2013 La Mancha  Ciudad Real Spain"},{"name":"Centre de Recherche\u00a0en Sciences Naturelles de Lwiro (CRSN\u00a0Lwiro), Lwiro  South Kivu Democratic Republic of Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8116-8491","authenticated-orcid":false,"given":"Krzysztof","family":"Anusz","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Public Health Protection Institute of Veterinary Medicine Warsaw University of Life Sciences (SGGW)  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0446-7682","authenticated-orcid":false,"given":"Jos\u00e9 Antonio","family":"Infantes\u2010Lorenzo","sequence":"additional","affiliation":[{"name":"Unidad de Inmunolog\u00eda Microbiana Centro Nacional de Microbiolog\u00eda Instituto de Salud Carlos III  Majadahonda Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0012-4006","authenticated-orcid":false,"given":"Christian","family":"Gort\u00e1zar","sequence":"additional","affiliation":[{"name":"SaBio Instituto de Investigaci\u00f3n en Recursos Cineg\u00e9ticos IREC Consejo Superior de Investigaciones Cient\u00edficas, Universidad de Castilla \u2013 La Mancha  Ciudad Real Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,9]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci4040065"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.00374"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40064\u2010016\u20102547\u20100"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2018.03.006"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2017.05.012"},{"key":"e_1_2_12_7_1","first-page":"45","article-title":"European bison restoration in Spain\u20149 Years of the work","volume":"12","author":"Castillo M. F.","year":"2019","journal-title":"European Bison Conservation Newsletter"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250\u2010018\u20101528\u20104"},{"key":"e_1_2_12_9_1","first-page":"78","article-title":"Commission Implementing Regulation (EU) 2021\/620 of 15 April 2021 laying down rules for the application of Regulation (EU) 2016\/429 of the European Parliament and of the Council as regards the approval of the disease\u2010free and non\u2010vaccination status of certain Member States or zones or compartments thereof as regards certain listed diseases and the approval of eradication programmes for those listed diseases (Text with EEA relevance)","volume":"131","author":"European Union","year":"2021","journal-title":"OJL"},{"issue":"3","key":"e_1_2_12_10_1","first-page":"1039","article-title":"A review of tests available for use in the diagnosis of tuberculosis in non\u2010bovine species","volume":"24","author":"Cousins D. V.","year":"2005","journal-title":"Revue Scientifique et Technique (International Office of Epizootics)"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13735"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/f13060920"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vas.2021.100174"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2020.108952"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.26444\/aaem\/130822"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11020260"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10112017"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11072094"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jhered\/esaa002"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2016.02.002"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2022.105612"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2708.210634"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2009.07.014"},{"issue":"6","key":"e_1_2_12_24_1","first-page":"593","article-title":"An outbreak of bovine tuberculosis in an intensively managed conservation herd of wild bison in the Northwest Territories","volume":"51","author":"Himsworth C. G.","year":"2010","journal-title":"The Canadian Veterinary Journal = La Revue Veterinaire Canadienne"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.134"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12014\u2010017\u20109171\u2010z"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010019\u20101814\u2010z"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2018.00189"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2018.02.003"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0021-8790.2004.00836.x"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2020.1868616"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090\u20103558\u201027.1.16"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.143926"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0228243"},{"key":"e_1_2_12_35_1","first-page":"842","article-title":"Bovine tuberculosis in bison (Bison bonasus caucasicus) located in Poland","volume":"10","author":"Krajewska M.","year":"2011","journal-title":"Post\u0119py Nauk Medycznych"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.7589\/2013\u201007\u2010187"},{"key":"e_1_2_12_37_1","unstructured":"Krajewska M. Kozi\u0144ska M. Or\u0142owska B. Augustynowicz\u2010Kope\u0107 E. Anusz K. Welz M. &Szulowski K.(2016).Mycobacterium bovis u \u017cubra w Puszczy Boreckiej.Materia\u0142y konferencyjne \u201c\u017bubry w Krainie Dinozaur\u00f3w\u201d 8\u20139 wrze\u015bnia 2016."},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani13071205"},{"key":"e_1_2_12_39_1","unstructured":"Krasi\u0144ska M. &Krasi\u0144ski Z. A.(2017).\u017bubr. Monografia Przyrodnicza. Wydawnictwo Chyra.pl."},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.2478\/jvetres\u20102022\u20100038"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2018.01.005"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.20506\/rst.21.2.1341"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9061257"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102334\u201010\u201046"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2003.12.010"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749\u20106632.2002.tb04385.x"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.7589\/2015\u201012\u2010329"},{"key":"e_1_2_12_48_1","volume-title":"European bison (Bison bonasus) strategic species status review 2020","author":"Olech W.","year":"2022"},{"key":"e_1_2_12_49_1","volume-title":"Podr\u0119cznik Najlepszych Praktyk Ochrony Zubra","author":"Olech W.","year":"2014"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2511.190746"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/acv.12530"},{"key":"e_1_2_12_52_1","volume-title":"Bison bonasus","author":"Plumb G.","year":"2020"},{"key":"e_1_2_12_53_1","volume-title":"European bison pedigree book 2021","author":"Raczynski J.","year":"2022"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250\u2010019\u201002151\u20102"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.20506\/rst.32.1.2203"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090\u20103558\u201037.1.101"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090\u20103558\u201045.3.729"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1912.130167"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03548"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090\u20103558\u201046.2.442"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.7589\/2014-06-167"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.7589\/2014-06-167"},{"issue":"4","key":"e_1_2_12_63_1","first-page":"672","article-title":"Brucella suis biovar 2 isolations from cattle in Poland","volume":"20","author":"Szulowski K.","year":"2013","journal-title":"Annals of Agricultural and Environmental Medicine: AAEM"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.2478\/pjvs\u20102013\u20100109"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9102084"},{"issue":"3","key":"e_1_2_12_66_1","first-page":"174","article-title":"A survey of brucellosis and tuberculosis in bison in and around Wood Buffalo National Park, Canada","volume":"31","author":"Tessaro S. V.","year":"1990","journal-title":"The Canadian Veterinary Journal = La Revue Veterinaire Canadienne"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2018.11.004"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2018.12.021"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567\u2010020\u201000881\u2010y"},{"key":"e_1_2_12_70_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0231559"},{"key":"e_1_2_12_71_1","first-page":"441","article-title":"Bovine tuberculosis in European bison in the Bieszczady region","volume":"61","author":"Welz M.","year":"2005","journal-title":"Medycyna Weterynaryjna"},{"issue":"11","key":"e_1_2_12_72_1","first-page":"1169","article-title":"Bovine tuberculosis in Canadian wildlife: An updated history","volume":"50","author":"Wobeser G.","year":"2009","journal-title":"The Canadian Veterinary Journal = La Revue Veterinaire Canadienne"},{"key":"e_1_2_12_73_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104\u2010017\u20102413\u20103"},{"key":"e_1_2_12_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2015.12.032"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.1314","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T09:34:36Z","timestamp":1710840876000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.1314"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,9]]},"references-count":73,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1002\/vms3.1314"],"URL":"https:\/\/doi.org\/10.1002\/vms3.1314","archive":["Portico"],"relation":{},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,9]]},"assertion":[{"value":"2022-10-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1314"}}