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Thus, detection of <jats:italic>M.\u00a0bovis<\/jats:italic>\u2010infected badgers may be required for the purposes of surveillance and monitoring of disease levels in infected populations. Current serological assays to detect <jats:italic>M.\u00a0bovis<\/jats:italic> infection in live badgers, while rapid and inexpensive, show limited diagnostic sensitivity. Here we describe and evaluate new <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> platforms for the recognition of the P22 multiprotein complex derived from the purified protein derivative (<jats:styled-content style=\"fixed-case\">PPD<\/jats:styled-content>) of <jats:italic>M.\u00a0bovis<\/jats:italic>. The recognition of IgG against P22 multiprotein complex derived from <jats:styled-content style=\"fixed-case\">PPD<\/jats:styled-content>\u2010B was tested by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> in the serum of badgers from the <jats:styled-content style=\"fixed-case\">UK<\/jats:styled-content>, Ireland and Spain. <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content> infection in the badgers was indicated by the presence of <jats:italic>M.\u00a0bovis<\/jats:italic> in tissues by culture and histology at post\u2010mortem examination and <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>\u2010free status was established by repeated negativity in the interferon <jats:italic>\u03b3<\/jats:italic> release assay (<jats:styled-content style=\"fixed-case\">IGRA<\/jats:styled-content>). In experimentally infected badgers, humoral antibody responses against P22 developed within 45\u00a0days post\u2010infection. The <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> tests showed estimated sensitivity levels of 74\u201382% in experimentally and naturally infected badgers with specificities ranging from 75% to 100% depending on the badger population tested. The P22 multi\u2010antigen based <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>s provide a sensitive and specific test platform for improved tuberculosis surveillance in badgers.<\/jats:p>","DOI":"10.1002\/vms3.134","type":"journal-article","created":{"date-parts":[[2019,1,18]],"date-time":"2019-01-18T08:33:57Z","timestamp":1547800437000},"page":"61-69","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["New serological platform for detecting antibodies against <i>Mycobacterium tuberculosis<\/i> complex in European badgers"],"prefix":"10.1002","volume":"5","author":[{"given":"Jose A.","family":"Infantes\u2010Lorenzo","sequence":"first","affiliation":[{"name":"VISAVET Health Surveillance Centre Universidad Complutense de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipesh","family":"Dave","sequence":"additional","affiliation":[{"name":"Bacteriology 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