{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:51:56Z","timestamp":1790351516602,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T00:00:00Z","timestamp":1705363200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Reported as being expressed by mono\u2010 and binucleate placental cells, pregnancy\u2010associated glycoproteins (PAGs) are released into the blood circulation from the ruminant placenta. Circulating gestational PAGs levels may differ between sheep breeds.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study was aimed at the close monitoring of the serum PAGs profiles of Karya and Konya Merino sheep during early pregnancy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Fifteen Karya and 15 Konya Merino ewes were synchronized by a 12\u2010day treatment with progesterone\u2010impregnated intravaginal sponges. After the sponges were withdrawn, the ewes were administered 400\u00a0IU of equine chorionic gonadotropin. The ewes were allowed to mate naturally, and all animals were sampled for blood as of the day of mating (day 0) at weeks 1, 2, 3, 4 and 5. Pregnancy diagnoses were made by transabdominal ultrasonography at week 5. At weeks 6, 7, 8, 9 and 11, blood samples were collected only from the pregnant ewes. The blood samples were centrifuged at 3000\u00a0\u00d7\u00a0\n                      <jats:italic>g<\/jats:italic>\n                      , and extracted sera were stored at \u221220\u00b0C until being used for laboratory analyses. Serum PAGs levels were determined with the aid of a commercial PAG\u2010enzyme\u2010linked immunosorbent assay test originally developed for pregnancy diagnosis in cattle. Differences in the between the PAGs levels throughout pregnancy and the group effect (Karya and Konya Merino) were determined with a two\u2010way mixed analysis of variance. Pairwise comparisons were made using a Bonferroni adjustment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>PAGs levels showed a linear increase with the advance of pregnancy in both Karya and Konya Merino sheep. No difference was detected between the breeds for serum PAGs levels. The serum PAGs levels of the pregnant and non\u2010pregnant ewes differed as of the fourth week.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The serum PAGs levels of the Karya and Konya Merino ewes were similar during the first 11 weeks of gestation, and pregnancy diagnosis could be made based on serum PAGs levels as of the 4th week in both breeds.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.1345","type":"journal-article","created":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T07:29:47Z","timestamp":1705390187000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Serum pregnancy\u2013associated glycoprotein profiles during early gestation in Karya and Konya Merino sheep"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7517-4927","authenticated-orcid":false,"given":"Mehmet","family":"Akk\u00f6se","sequence":"first","affiliation":[{"name":"Department of Livestock Dalaman Agricultural Enterprise General Directorate of Agricultural Enterprises  Mu\u011fla Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0684-7213","authenticated-orcid":false,"given":"Elif Merve","family":"\u00c7\u0131nar","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination Faculty of Veterinary Medicine Dicle University  Diyarbak\u0131r Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0039-5597","authenticated-orcid":false,"given":"Murat Onur","family":"Yazl\u0131k","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Faculty of Veterinary Medicine Ankara University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4805-0993","authenticated-orcid":false,"given":"Ufuk","family":"Kaya","sequence":"additional","affiliation":[{"name":"Department of Biostatistics Faculty of Veterinary Medicine Hatay Mustafa Kemal University  Hatay Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5603-2149","authenticated-orcid":false,"given":"Yadigar","family":"Polat","sequence":"additional","affiliation":[{"name":"Department of Medical Services and Techniques Vocational High School of Health Services Kilis 7 Aral\u0131k University  Kilis Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6876-2069","authenticated-orcid":false,"given":"\u00c7i\u011fdem","family":"\u00c7ebi","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination Faculty of Veterinary Medicine Harran University  \u015eanl\u0131urfa Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3748-9992","authenticated-orcid":false,"given":"Ceyhan","family":"\u00d6zbeyaz","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences Faculty of Veterinary Medicine Ankara University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7252-7977","authenticated-orcid":false,"given":"Mehmet R\u0131fat","family":"Vural","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Faculty of Veterinary Medicine Ankara University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2478\/macvetrev-2021-0010"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2022.106722"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.18016\/ksutarimdoga.vi.668707"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2023.01.022"},{"issue":"2","key":"e_1_2_9_6_1","first-page":"1","article-title":"Pregnancy\u2010associated glycoproteins (PAGs) and progesterone concentrations in Holstein heifers following two methods of estrus synchronization","volume":"6","author":"Akkose M.","year":"2019","journal-title":"Veterinaria M\u00e9xico"},{"issue":"11","key":"e_1_2_9_7_1","first-page":"563","article-title":"Pregnancy\u2010associated glycoproteins concentrations during early gestation in pregnant Awassi sheep","volume":"77","author":"Akk\u00f6se M.","year":"2021","journal-title":"Medycyna Weterynaryjna"},{"issue":"1","key":"e_1_2_9_8_1","first-page":"25","article-title":"Determination of pregnancy protein levels to distinguish between singleton and twin pregnancies in Awassi sheep","volume":"78","author":"Akku\u015f T.","year":"2022","journal-title":"Medycyna Weterynaryjna"},{"key":"e_1_2_9_9_1","unstructured":"Alabart J. 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