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Antibiotic resistance of\n                      <jats:italic>S. aureus<\/jats:italic>\n                      in raw milk can increase the risk of such infections, particularly among susceptible individuals.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study aimed to investigate the prevalence of enterotoxin genes\n                      <jats:italic>a<\/jats:italic>\n                      ,\n                      <jats:italic>d<\/jats:italic>\n                      ,\n                      <jats:italic>g<\/jats:italic>\n                      ,\n                      <jats:italic>i<\/jats:italic>\n                      and\n                      <jats:italic>j<\/jats:italic>\n                      and the antibiotic resistance of\n                      <jats:italic>S. aureus<\/jats:italic>\n                      isolated from raw milk samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      During a 6\u2010month sampling period, 60 raw milk specimens were obtained from diverse locations in Yazd province, Iran. Antibiogram profiling was conducted via the disc diffusion method. In addition, staphylococcal enterotoxin (SE) genes\n                      <jats:italic>a<\/jats:italic>\n                      ,\n                      <jats:italic>d<\/jats:italic>\n                      ,\n                      <jats:italic>g<\/jats:italic>\n                      ,\n                      <jats:italic>i<\/jats:italic>\n                      , and\n                      <jats:italic>j<\/jats:italic>\n                      were detected through real\u2010time PCR analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Bacteriological assays confirmed the presence of\n                      <jats:italic>S. aureus<\/jats:italic>\n                      in 11 samples (18.3%). All isolates demonstrated 100% resistance to penicillin G but exhibited sensitivity to vancomycin, while resistance to other antibiotics ranged from 36.4% to 45.5%. The prevalence of enterotoxin genes in these strains showed variable distribution, with\n                      <jats:italic>sea<\/jats:italic>\n                      being the predominant SE (45.5%), followed by\n                      <jats:italic>sed<\/jats:italic>\n                      (36.4%),\n                      <jats:italic>seg<\/jats:italic>\n                      (18.2),\n                      <jats:italic>sej<\/jats:italic>\n                      and\n                      <jats:italic>sei<\/jats:italic>\n                      (9.1% each).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study discovered the presence of multiple enterotoxins in\n                      <jats:italic>S. aureus<\/jats:italic>\n                      strains obtained from raw milk samples. These strains also demonstrated resistance to a variety of antibiotics. Since enterotoxigenic\n                      <jats:italic>S. aureus<\/jats:italic>\n                      is known to cause human food poisoning, monitoring food hygiene practices, especially during raw milk production, is critical.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.1407","type":"journal-article","created":{"date-parts":[[2024,3,23]],"date-time":"2024-03-23T00:23:55Z","timestamp":1711153435000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Rapid detection of major enterotoxin genes and antibiotic resistance of\n                    <i>Staphylococcus aureus<\/i>\n                    isolated from raw milk in the Yazd province, Iran"],"prefix":"10.1002","volume":"10","author":[{"given":"Mohamad Javad","family":"Forouzani\u2010Moghaddam","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Sciences Faculty of Allied Medicine Iran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1183-8549","authenticated-orcid":false,"given":"Sina","family":"Habibi","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences Faculty of Allied Medicine Iran University of Medical Sciences Tehran Iran"},{"name":"Department of Hematology and Blood Banking, Faculty of Allied Medicine Iran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1581-3402","authenticated-orcid":false,"given":"Ahmad","family":"Hosseini\u2010Safa","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences Faculty of Allied Medicine Iran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khadijeh","family":"Khanaliha","sequence":"additional","affiliation":[{"name":"Research Center of Pediatric Infectious Diseases Institute of Immunology and Infectious Diseases Iran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roya","family":"Mokarinejad","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences Faculty of Allied Medicine Iran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Akhoundzadeh","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences Faculty of Allied Medicine Iran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mojgan","family":"Oshaghi","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences Faculty of Allied Medicine Iran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/BF03547695"},{"issue":"4","key":"e_1_2_10_3_1","first-page":"229","article-title":"Enterotoxigenic Staphylococcus aureus in raw milk in the North of Palestine","volume":"29","author":"Adwan G. 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