{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T08:47:50Z","timestamp":1773132470321,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,1,14]],"date-time":"2019-01-14T00:00:00Z","timestamp":1547424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["2016YFD0501105"],"award-info":[{"award-number":["2016YFD0501105"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["2016YFC1201603\u2010X"],"award-info":[{"award-number":["2016YFC1201603\u2010X"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Porcine circovirus type 3 (<jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>3) is a novel pathogen first identified in the United States in 2016. As there is a high possibility that no clinical signs of infection are observed in the host, an accurate and sensitive method is needed for quarantine on numerous live pigs especially for international pig trade. In this study, a TaqMan\u2010based real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assay specifically for <jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>3 was established without cross\u2010reactions with other non\u2010targeted pig viruses. The sensitivity of the current approach is about 1.5\u00a0\u00d7\u00a010<jats:sup>1\u00a0<\/jats:sup>copies\u00a0<jats:italic>\u03bc<\/jats:italic>L<jats:sup>\u22121<\/jats:sup> plasmid <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> while the sensitivity of the conventional <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> is about 1.5\u00a0\u00d7\u00a010<jats:sup>2<\/jats:sup>\u00a0copies\u00a0<jats:italic>\u03bc<\/jats:italic>L<jats:sup>\u22121<\/jats:sup> plasmid <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. Further, this assay was applied in the retrospective quarantine on serum samples of 601 commercial live boars imported to China from the United States, France and the United Kingdom from 2011 to 2017. The results revealed that <jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>3 could be detected positive in the commercial boars imported from the United States and the above\u2010mentioned western European countries and phylogenetic study also revealed that viral isolates were grouped with some isolates from Korea and the United States. Our study suggested that <jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>3 may be prevalent globally since 2011.<\/jats:p>","DOI":"10.1002\/vms3.141","type":"journal-article","created":{"date-parts":[[2019,1,14]],"date-time":"2019-01-14T10:39:00Z","timestamp":1547462340000},"page":"168-175","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Establishment of a sensitive TaqMan\u2010based real\u2010time <scp>PCR<\/scp> assay for porcine circovirus type 3 and its application in retrospective quarantine of imported boars to China"],"prefix":"10.1002","volume":"5","author":[{"given":"Chunyan","family":"Feng","sequence":"first","affiliation":[{"name":"Institute of Animal Quarantine Chinese Academy of Inspection and Quarantine Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CaiXia","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Animal 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