{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T12:55:08Z","timestamp":1759496108140,"version":"3.41.2"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T00:00:00Z","timestamp":1716854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010263","name":"Bing\u00f6l \u00dcniversitesi","doi-asserted-by":"publisher","award":["Project No: BAP\u2010VF.2022.001"],"award-info":[{"award-number":["Project No: BAP\u2010VF.2022.001"]}],"id":[{"id":"10.13039\/501100010263","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The cestode <jats:italic>Taenia hydatigena<\/jats:italic> uses canids, primarily dogs, as definitive hosts, whereas the metacestode larval stage cysticercus infects a range of intermediate hosts, including domestic animals such as goats, sheep and pigs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>In this study, we aimed to determine the genetic differences and haplotypes of <jats:italic>Cysticercus tenuicollis<\/jats:italic> isolates obtained from sheep and goats slaughtered in the Bingol and Elazig provinces of Turkey.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>C. tenuicollis<\/jats:italic> isolates were collected from 44 sheep and 26 goats slaughtered in slaughterhouses in Bingol and Elazig. After the isolation of total genomic DNA from <jats:italic>C. tenuicollis<\/jats:italic> isolates, the genetic characterization of the partial mitochondrial cytochrome oxidase 1 (CO1) gene region (866\u00a0bp) was amplified using specific primers by polymerase chain reaction (PCR), the products were then sequenced, and haplotype and genetic diversity analyses were carried out.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>As a result of the haplotype network analyses, 34 different haplotypes were detected around the main haplotype (Hap02) arranged in a star\u2010like configuration and separated from other haplotypes by 1\u201328 mutation steps and covering 22.85% (16\/70) of all isolates. Twenty\u2010seven polymorphic fields were detected, 77.77% (21\/27) of which were parsimony\u2010informative, and secondary haplotype and nucleotide diversity were observed. Additionally, we detected high intraspecies haplotype diversity (hd: 0.933) and high nucleotide diversity (<jats:italic>\u03c0<\/jats:italic>: 0.00383), with 27 different nucleotide variation positions among the haplotypes of the isolates. Tajima's <jats:italic>D<\/jats:italic> value was negative, indicating population expansion and\/or selection purification. The significantly negative Fu's Fs values indicated recent population expansion or the presence of expected rare haplotypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results of this study confirmed that <jats:italic>C. tenuicollis<\/jats:italic> isolates clustered in one lineage and were closely related to the relevant reference sequences in different countries, confirming the circulation of <jats:italic>C. tenuicollis<\/jats:italic> in different geographical regions.<\/jats:p><\/jats:sec>","DOI":"10.1002\/vms3.1411","type":"journal-article","created":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T12:10:28Z","timestamp":1716898228000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Genetic diversity and haplotypes of <i>Cysticercus tenuicollis<\/i> isolates from slaughtered sheep and goats in Elazig and Bingol provinces of Turkey"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8410-0307","authenticated-orcid":false,"given":"Sirvan","family":"Karakoc","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine University of Bingol  Bingol Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8480-8597","authenticated-orcid":false,"given":"Harun Kaya","family":"Kesik","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine University of Bingol  Bingol Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2188-0196","authenticated-orcid":false,"given":"Figen","family":"Celik","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine University of Firat  Elaz\u0131g Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1769-4558","authenticated-orcid":false,"given":"Muhammed Ahmed","family":"Selcuk","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine University of Siirt  Siirt Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8454-1901","authenticated-orcid":false,"given":"Seyma","family":"Gunyakti Kilinc","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine University of Bingol  Bingol Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3567-326X","authenticated-orcid":false,"given":"Sami","family":"Simsek","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine University of Firat  Elaz\u0131g 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