{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T20:09:32Z","timestamp":1789157372431,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:00:00Z","timestamp":1711065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["NU23\u201005\u201000511"],"award-info":[{"award-number":["NU23\u201005\u201000511"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hippoboscid flies are bloodsucking arthropods that can transmit pathogenic microorganisms and are therefore potential vectors for pathogens such as<jats:italic>Bartonella<\/jats:italic>spp. These Gram\u2010negative bacteria can cause mild\u2010to\u2010severe clinical signs in humans and animals; therefore, monitoring<jats:italic>Bartonella<\/jats:italic>spp. prevalence in louse fly populations appears to be a useful prerequisite for zoonotic risk assessment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using convenience sampling, we collected 103 adult louse flies from four ked species (<jats:italic>Lipoptena cervi<\/jats:italic>,<jats:italic>n<\/jats:italic>\u00a0=\u00a022;<jats:italic>Lipoptena fortisetosa<\/jats:italic>,<jats:italic>n<\/jats:italic>\u00a0=\u00a061;<jats:italic>Melophagus ovinus<\/jats:italic>,<jats:italic>n<\/jats:italic>\u00a0=\u00a012;<jats:italic>Hippobosca equina<\/jats:italic>,<jats:italic>n<\/jats:italic>\u00a0=\u00a08) and the pupae of<jats:italic>M. ovinus<\/jats:italic>(<jats:italic>n<\/jats:italic>\u00a0= 10) in the federal state of Saxony, Germany. All the samples were screened by polymerase chain reaction (PCR) for<jats:italic>Bartonella<\/jats:italic>spp. DNA, targeting the citrate synthase gene (<jats:italic>gltA<\/jats:italic>). Subsequently, PCRs targeting five more genes (16S,<jats:italic>ftsZ<\/jats:italic>,<jats:italic>nuoG<\/jats:italic>,<jats:italic>ribC<\/jats:italic>and<jats:italic>rpoB<\/jats:italic>) were performed for representatives of revealed<jats:italic>gltA<\/jats:italic>genotypes, and all the PCR products were sequenced to identify the<jats:italic>Bartonella<\/jats:italic>(sub)species accurately.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and Conclusions<\/jats:title><jats:p>The overall detection rates for<jats:italic>Bartonella<\/jats:italic>spp. were 100.0%, 59.1%, 24.6% and 75.0% in<jats:italic>M. ovinus<\/jats:italic>,<jats:italic>L. cervi<\/jats:italic>,<jats:italic>L. fortisetosa<\/jats:italic>and<jats:italic>H. equina<\/jats:italic>, respectively. All the identified bartonellae belong to the<jats:italic>Bartonella schoenbuchensis<\/jats:italic>complex. Our data support the proposed reclassification of the (sub)species status of this group, and thus we conclude that several genotypes of<jats:italic>B. schoenbuchensis<\/jats:italic>were detected, including<jats:italic>Bartonella schoenbuchensis<\/jats:italic>subsp.<jats:italic>melophagi<\/jats:italic>and<jats:italic>Bartonella schoenbuchensis<\/jats:italic>subsp.<jats:italic>schoenbuchensis<\/jats:italic>, both of which have previously validated zoonotic potential. The extensive PCR analysis revealed the necessity of multiple PCR approach for proper identification of the ruminant\u2010associated bartonellae.<\/jats:p><\/jats:sec>","DOI":"10.1002\/vms3.1417","type":"journal-article","created":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T09:24:48Z","timestamp":1711099488000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Detection of<i>Bartonella schoenbuchensis<\/i>(sub)species DNA in different louse fly species in Saxony, Germany: The proof of multiple PCR analysis necessity in case of ruminant\u2010associated bartonellae determination"],"prefix":"10.1002","volume":"10","author":[{"given":"Isabelle","family":"Vogt","sequence":"first","affiliation":[{"name":"Faculty of Agriculture\/Environment\/Chemistry HTW Dresden \u2013 University of Applied Sciences Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Schr\u00f6ter","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture\/Environment\/Chemistry HTW Dresden \u2013 University of Applied Sciences Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruben","family":"Schreiter","sequence":"additional","affiliation":[{"name":"ZAFT e.V. \u2013 Centre for Applied Research and Technology Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hein","family":"Sprong","sequence":"additional","affiliation":[{"name":"Laboratory for Zoonoses and Environmental Microbiology National Institute for Public Health and the Environment (RIVM) Bilthoven The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Volfov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Parasitology Faculty of Science, Charles University Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Jentzsch","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture\/Environment\/Chemistry HTW Dresden \u2013 University of Applied Sciences Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2695-8316","authenticated-orcid":false,"given":"Markus","family":"Freick","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture\/Environment\/Chemistry HTW Dresden \u2013 University of Applied Sciences Dresden Germany"},{"name":"ZAFT e.V. \u2013 Centre for Applied Research and Technology Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03159-14"},{"key":"e_1_2_9_3_1","unstructured":"Baltes\u2010G\u00f6tz B.(2012).Logistische Regressionsanalyse mit SPSS.SPSS."},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-46076-4"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects12060485"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2020.105521"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2019.05.010"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-0764-y"},{"key":"e_1_2_9_9_1","unstructured":"B\u00fcttiker W.(1994).Die Lausfliegen der Schweiz (Diptera Hippoboscidae): Mit Bestimmungsschl\u00fcssel(Documenta faunistica Helvetiae).Centre suisse de cartographie de la faune.https:\/\/books.google.de\/books?id=BsuePQAACAAJ"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2003.tb07376.x"},{"issue":"7","key":"e_1_2_9_11_1","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.3201\/eid1007.030896","article-title":"Bartonella spp. DNA associated with biting flies from California","volume":"10","author":"Chung C. 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