{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:17:20Z","timestamp":1790867840085,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T00:00:00Z","timestamp":1713916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The most prevalent probiotic bacterium employed in the food industry is\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      because it can produce metabolites with antibacterial capabilities and exhibits hostility towards infections and microorganisms that cause spoilage.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      This study set out to identify naturally occurring\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      and plantaricin\n                      <jats:italic>(pln EF)<\/jats:italic>\n                      coding genes in raw cow milk and to assess the antibacterial potency of isolated\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Following enrichment in De Man, Rogosa and Sharpe (MRS) broth, single colonies were isolated, and pure colonies were obtained by streaking on MRS agar. The\n                      <jats:italic>16S rRNA<\/jats:italic>\n                      gene was amplified using polymerase chain reaction (PCR) to confirm the cultural positivity of all isolates. Additionally, the presence of plantaricin was verified by targeting the\n                      <jats:italic>pln EF<\/jats:italic>\n                      gene through PCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Outcome<\/jats:title>\n                    <jats:p>\n                      Out of the 166 raw milk specimens acquired from cows, 153 (91.17%; CI: 86.98\u201395.76) were identified as positive for\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      through both culture and biochemical screening. Subsequently, 121 (72.89%; CI: 65.46\u201379.49) of the isolates were affirmed to harbour\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      through PCR analysis. Within this subset, 6 isolates (4.96%; CI: 1.84\u201310.48) were found to possess the\n                      <jats:italic>plnEF<\/jats:italic>\n                      gene. When exposed to Lactobacillus isolates,\n                      <jats:italic>Salmonella Typhimurium<\/jats:italic>\n                      and\n                      <jats:italic>Salmonella enterica<\/jats:italic>\n                      displayed an average maximum zone of inhibition with a diameter measuring 24\u00a0mm. In contrast,\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      exhibited an average minimum zone of inhibition, featuring a diameter of 11\u00a0mm. Additionally, the\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      isolates demonstrated inhibitory zones against\n                      <jats:italic>Staphylococcus aureus, Klebsiella pneumoniae<\/jats:italic>\n                      and\n                      <jats:italic>Klebsiella oxytoca<\/jats:italic>\n                      , measuring 14, 22 and 19\u00a0mm, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical significance<\/jats:title>\n                    <jats:p>Lactic acid bacteria, particularly Lactobacilli, are plentiful in cow milk and possess broad\u2010spectrum antibacterial properties.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.1463","type":"journal-article","created":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T02:53:16Z","timestamp":1714013596000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Antibacterial potential of lactic acid bacteria isolated from raw cow milk in Sylhet district, Bangladesh: A molecular approach"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1431-3808","authenticated-orcid":false,"given":"Mashuka Nahida","family":"Asha","sequence":"first","affiliation":[{"name":"Department of Medicine Faculty of Veterinary Animal and Biomedical Sciences Sylhet Agricultural University  Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0210-6581","authenticated-orcid":false,"given":"Md. 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Anisur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Medicine Faculty of Veterinary Animal and Biomedical Sciences Sylhet Agricultural University  Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4426-3004","authenticated-orcid":false,"given":"Ahsan Al","family":"Emon","sequence":"additional","affiliation":[{"name":"Department of Medicine Faculty of Veterinary Animal and Biomedical Sciences Sylhet Agricultural University  Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0202-2727","authenticated-orcid":false,"given":"Fatema Yeasmin","family":"Tanni","sequence":"additional","affiliation":[{"name":"Department of Medicine Faculty of Veterinary Animal and Biomedical Sciences Sylhet Agricultural University  Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9100-7632","authenticated-orcid":false,"given":"Md. 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