{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T09:50:50Z","timestamp":1784713850263,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,6,16]],"date-time":"2024-06-16T00:00:00Z","timestamp":1718496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>This study presents the case of non\u2010purulent encephalomyelitis associated with astrovirus infection in a sheep from Eastern Anatolia, T\u00fcrkiye.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A necropsy was performed on a sheep showing nervous signs. Afterwards, brain tissue samples were taken and examined with histopathological, immunohistochemical and molecular techniques.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Neuropathologic changes included neuronal degeneration, diffuse gliosis, multifocal perivascular cuffing, neuronophagy and neuronal necrosis in the cerebrum, the cerebellum and the cervical spinal cord. Aerobic and anaerobic bacterial culture, selective culture for\n                      <jats:italic>Listeria monocytogenes<\/jats:italic>\n                      , and PCR analysis for rabies virus, tick\u2010borne encephalitis virus, T\u00fcrkiye encephalitis virus, small ruminant lentiviruses and border disease virus were negative. However, the presence of astrovirus RNA in cerebral, cerebellar and spinal cord samples was demonstrated by a pan\u2010astrovirus RT\u2010PCR. Immunohistochemical examinations revealed astrovirus antigens within the neuronal cytoplasm. High\u2010throughput sequencing techniques identified the causative agent as a member of the genotype species\n                      <jats:italic>Mamastrovirus<\/jats:italic>\n                      13 but representing a distinct genetic lineage with similarity to ovine astrovirus 1 in the open\u2010reading frames (ORF)1ab region and muskox astrovirus in the ORF2 region.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This report provides evidence that astroviruses are potentially encephalitis\u2010causing pathogens in ovine populations in T\u00fcrkiye, featuring an astrovirus strain distinct from those previously identified in sheep.<\/jats:p>\n                  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