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It was first discovered in Iran between 1967 and 1976 in small ruminants, equines, environments and humans. No subsequent studies have been conducted to determine the existence and prevalence of this pathogen in the country.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      The present study aims to monitor the presence of\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      in the ruminant population of the Golestan province of Iran, which largely depends on pastures. The ruminants can serve as sentinels to indicate the presence of the bacteria in the environment and its potential impact on human health in the One Health triad.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Liver and lung abscesses from domestic sheep, cattle and goats in three industrial and three conventional slaughterhouses were sampled and analysed using 23S ribosomal DNA polymerase chain reaction (rDNA PCR) with primers CVMP 23\u20101 and CVP\u201023\u20102 for\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      ,\n                      <jats:italic>Burkholderia cepacia<\/jats:italic>\n                      and\n                      <jats:italic>Burkholderia vietnamiensis<\/jats:italic>\n                      , as well as\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      \u2013specific TTS1 real\u2010time PCR, along with microbiological and biochemical assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Out of the 97 animals sampled, only 14 (15%) tested positive for 23S rDNA PCR. However, the follow\u2010up evaluation using TTS1 real\u2010time PCR and microbiological and biochemical assays did not confirm the presence of\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      in the samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Although\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      was not detected in the current survey, conducting abattoir\u2010based surveillance of ruminants is a cost\u2010effective One Health approach to monitor pathogenic\n                      <jats:italic>Burkholderia<\/jats:italic>\n                      . Developing standards of clinical and laboratory good practices for\n                      <jats:italic>Burkholderia<\/jats:italic>\n                      infections is crucial for One Health surveillance.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.1503","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T13:52:48Z","timestamp":1719323568000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Tracking melioidosis in Iran: Utilizing abattoir\u2010based surveillance as a One Health approach"],"prefix":"10.1002","volume":"10","author":[{"given":"Nader","family":"Mosavari","sequence":"first","affiliation":[{"name":"Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO) Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1174-4761","authenticated-orcid":false,"given":"Mohsen","family":"Bashashati","sequence":"additional","affiliation":[{"name":"Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO) Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahdi","family":"Dehghanpour","sequence":"additional","affiliation":[{"name":"Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO) Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohsen","family":"Abdolvand","sequence":"additional","affiliation":[{"name":"Iran Veterinary Organization  Gorgan Golestan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faezeh","family":"Heshmatinia","sequence":"additional","affiliation":[{"name":"Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO) Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fereshteh","family":"Sabouri","sequence":"additional","affiliation":[{"name":"Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO) Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shojaat","family":"Dashtipour","sequence":"additional","affiliation":[{"name":"Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO) Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeid Mohammad","family":"Hosseini","sequence":"additional","affiliation":[{"name":"Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO) Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Najafpour","sequence":"additional","affiliation":[{"name":"Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO) Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7959-4718","authenticated-orcid":false,"given":"Zahra","family":"Baradaran\u2010Seyed","sequence":"additional","affiliation":[{"name":"Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO) Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,25]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","first-page":"209","article-title":"Equine melioidosis in Iran","volume":"22","author":"Baharsefat M.","year":"1970","journal-title":"Archives of Razi Institute"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.34172\/ijtmgh.2020.16"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.9.2737-2741.1998"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.3.990-994.2001"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S299797"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed3010013"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-024-03957-5"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0399-077X(76)80123-0"},{"issue":"1","key":"e_1_2_10_10_1","first-page":"42","article-title":"Isolement du bacille de Whitmore a partir de lesions rencontrees chez le porc a l'abattoir de","volume":"66","author":"Ferry R.","year":"1973","journal-title":"Niamey au Niger Bull Soc Pathol Exot"},{"key":"e_1_2_10_11_1","first-page":"3","article-title":"R\u00e9partition de Pseudomonas pseudomallei en\u00a0France et dans le monde","volume":"66","author":"Galimand M.","year":"1982","journal-title":"Bulletin Mensuel de la Soci\u00e9t\u00e9 V\u00e9t\u00e9rinaire Pratique de France"},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","unstructured":"Gasque M. 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