{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:31:27Z","timestamp":1792801887307,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T00:00:00Z","timestamp":1720051200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to examine the effects of supplementation of vitamin D to the egg\u2010yolk extender on characteristics of frozen\u2013thawed ram semen.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Semen samples obtained from adult rams were pooled and divided into five equal volumes. It was reconstituted with extenders containing different concentrations of vitamin D: 0 (control), 12.5 (VITD 12.5), 25 (VITD 25), 50 (VITD 50), and 100\u00a0ng\/mL (VITD 100), and then they were frozen. Sperm motility parameters, plasma membrane functional integrity, acrosomal integrity, DNA fragmentation, and mitochondrial membrane potential of the groups were evaluated after sperm thawing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Total motility and progressive motility were higher in VITD 50 than in all other groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Higher sperm straightness, linearity, and wooble were higher in VITD 50 than in the control group (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). A similar pattern of VITD 50 was observed for plasma membrane integrity and mitochondrial membrane potential (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&gt;\u00a00.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In the study, it was observed that adding vitamin D to the extender had a beneficial effect on ram spermatological parameters. In addition, it was concluded that the use of the 50\u00a0ng\/mL vitamin D in the extender provided more effective protection than the other doses.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.1526","type":"journal-article","created":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T07:59:12Z","timestamp":1720079952000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The effects of supplementation of vitamin D to the egg\u2010yolk extender on cryopreservation of ram semen"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2975-2594","authenticated-orcid":false,"given":"Ahmet","family":"Aktar","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination Bursa Uludag University  Bursa 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