{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:15:38Z","timestamp":1772118938447,"version":"3.50.1"},"reference-count":76,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T00:00:00Z","timestamp":1719878400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Acute flaccid paralysis (AFP) is a complex clinical syndrome with various aetiologies. If untreated, AFP may lead to death due to failure of respiratory muscles. Tick paralysis, which is a noninfectious neurologic syndrome of AFP, occurs following tick attachment, engorgement, and injection of tick saliva toxins. There is no specific diagnostic test for tick paralysis, and mortality increases as definitive diagnosis is delayed. Although metabolomic investigation of tick saliva was conducted, there is a lack of research on metabolomic evaluation of hosts affected by tick paralysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Thus, the aim of this study is to investigate metabolomic changes in serum samples of dogs with tick paralysis due to\n                      <jats:italic>Rhipicephalus sanguineus<\/jats:italic>\n                      using NMR\u2010based metabolomics and to identify potential diagnostic\/prognostic markers.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Forty dogs infested with\n                      <jats:italic>R. sanguineus<\/jats:italic>\n                      , with clinical findings compatible with AFP and with a confirmed tick paralysis diagnosis ex juvantibus, constituted the Paralysis Group. Ten healthy dogs, which were admitted either for vaccination and\/or check\u2010up purposes, constituted the Control Group. After the confirmation tick paralysis, medical history, vaccination and nutritional status, body surface area and estimated tick numbers of all the dogs were noted. Physical examination included body temperature, heart and respiratory rate, capillary refill time evaluation and Modified Glasgow Coma Scale calculation. Serum samples were extracted from venous blood samples of all the dogs and were prepared for NMR analysis, and NMR\u2010based metabolomics identification and quantification were performed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>NMR\u2010based serum metabolomics of the present study revealed distinct up\/down\u2010regulated expressions, presenting a promising avenue. Moreover, it was observed that energy metabolism and especially liver functions were impaired in dogs with tick paralysis, and not only the respiratory system but also the kidneys were affected.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>It was concluded that the present approach may help to better understand the pathological mechanisms developing in cases of AFP due to tick paralysis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.1528","type":"journal-article","created":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T03:25:02Z","timestamp":1719890702000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["NMR\u2010based metabolomic investigation of dogs with acute flaccid paralysis due to tick paralysis"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8511-0150","authenticated-orcid":false,"given":"Erdem","family":"G\u00fclersoy","sequence":"first","affiliation":[{"name":"Veterinary Faculty Department of Internal Medicine Harran University  \u015eanl\u0131urfa Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7473-5163","authenticated-orcid":false,"given":"Canberk","family":"Bal\u0131k\u00e7\u0131","sequence":"additional","affiliation":[{"name":"Veterinary Faculty Department of Internal Medicine Harran University  \u015eanl\u0131urfa Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4779-0893","authenticated-orcid":false,"given":"Adem","family":"\u015eahan","sequence":"additional","affiliation":[{"name":"Veterinary Faculty Department of Internal Medicine Harran University  \u015eanl\u0131urfa Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3679-4132","authenticated-orcid":false,"given":"\u0130smail","family":"G\u00fcnal","sequence":"additional","affiliation":[{"name":"Veterinary Faculty Department of Internal Medicine Harran University  \u015eanl\u0131urfa Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9853-5334","authenticated-orcid":false,"given":"Mehmet Osman","family":"Atl\u0131","sequence":"additional","affiliation":[{"name":"Veterinary Faculty Department of Reproduction and Artificial Insemination Harran University  \u015eanl\u0131urfa Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2013.0065"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/15563659009038588"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/adb.12571"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002\u20109343(61)90091\u20102"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2019.1707907"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-016-9866-6"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-64142-7.00052-7"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M407120200"},{"key":"e_1_2_11_10_1","first-page":"303","article-title":"Biomarkers in clinical medicine","author":"Chen X. 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