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The pooled sperm were divided into nine different groups: control (Tris\u2010based extender); 0.4, 0.6, 0.8 and 1\u00a0mM rutin (rutin\u00a0+\u00a0Tris\u2010based extender); and 50, 100, 150 and 200\u00a0\u00b5M CGG (CGA\u00a0+\u00a0Tris\u2010based extender). Sperm kinematics, viability, hypo\u2010osmotic swelling test, mitochondrial activity, antioxidant activities and malondialdehyde (MDA) concentration of frozen and thawed buffalo sperm were evaluated. In addition, 48 buffalo were finally inseminated, and pregnancy was rectally determined 1 month after insemination.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Compared to the control group, adding R\u20100.4, R\u20100.6, CGA\u2010100 and CGA\u2010150 can improve total and progressive motility, motility characteristics, viability, PMF and DNA damage in buffalo sperm. In addition, the results showed that R\u20100.4, R\u20100.6, CGA\u201050, CGA\u2010100 and CGA\u2010150 increased total antioxidant capacity, catalase, glutathione peroxidase and glutathione activities and decreased MDA levels compared to the control group. Furthermore, it has been shown that adding 150\u00a0\u00b5M CGA and 0.6\u00a0mM rutin to an extender can increase in vivo fertility compared to the control group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In conclusion, adding rutin and CGA to the extender improves membrane stability and in vivo fertility of buffalo sperm by reducing oxidative stress.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.1548","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T11:54:41Z","timestamp":1724068481000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The influence of rutin and chlorogenic acid on oxidative stress and in vivo fertility: Evaluation of the quality and antioxidant status of post\u2010thaw semen from Azari water buffalo bulls"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9147-9371","authenticated-orcid":false,"given":"Tohid","family":"Mohammadi","sequence":"first","affiliation":[{"name":"Department of Basic Science, Faculty of Veterinary Medicine Urmia Branch, Islamic Azad University  Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8627-2178","authenticated-orcid":false,"given":"Mohammadreza","family":"Hosseinchi Gharehaghaji","sequence":"additional","affiliation":[{"name":"Department of Basic Science, Faculty of Veterinary Medicine Urmia Branch, Islamic Azad University  Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,19]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.05.010"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.10.024"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.05.012"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12593"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080420311"},{"issue":"4","key":"e_1_2_12_7_1","first-page":"491","article-title":"Partial role of multiple pathways in infarct size limiting effect of quercetin and rutin against cerebral ischemia\u2010reperfusion injury in rats","volume":"17","author":"Annapurna A.","year":"2013","journal-title":"European Review for Medical & Pharmacological Sciences"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbio.2012.10.001"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.2.354"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/19396368.2023.2198070"},{"key":"e_1_2_12_11_1","unstructured":"Ashabi M. 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