{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T13:54:02Z","timestamp":1781877242553,"version":"3.54.5"},"reference-count":56,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T00:00:00Z","timestamp":1723075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T00:00:00Z","timestamp":1723075200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003121","name":"Ferdowsi University of Mashhad","doi-asserted-by":"publisher","award":["3\/57427"],"award-info":[{"award-number":["3\/57427"]}],"id":[{"id":"10.13039\/501100003121","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background\/Objective<\/jats:title>\n                    <jats:p>\n                      The cestode\n                      <jats:italic>Echinococcus granulosus<\/jats:italic>\n                      causes cystic echinococcosis, a zoonotic parasitic infection that constitutes a significant public health risk. This parasite has been documented to have potential reservoirs and carriers among wild canids, namely wolves, foxes and jackals. This study aimed to determine the prevalence and molecular characteristics of\n                      <jats:italic>E. granulosus sensu lato<\/jats:italic>\n                      species\/genotypes among wild canids in three northern, northeastern and north\u2010western Iran regions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      From 2019 to 2022, 93 wild canid carcasses (69 jackals), (22 foxes) and (2 wolves) were collected that were killed in car accidents or illnesses. Analyses of morphology and morphometry were performed to verify the presence of\n                      <jats:italic>E. granulosus<\/jats:italic>\n                      . To determine\n                      <jats:italic>E. granulosus s.l<\/jats:italic>\n                      . species\/genotypes, polymerase chain reaction (PCR)\u2010RFLP (ITS1) was performed utilizing the Bsh1236I (BstUI) restriction enzyme.\n                      <jats:italic>COX1<\/jats:italic>\n                      ,\n                      <jats:italic>NADH1<\/jats:italic>\n                      and\n                      <jats:italic>ITS1<\/jats:italic>\n                      gene sequencing were also performed to confirm the PCR\u2010RFLP results.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      During this study, 93 wild canids were examined, and 3.2% (95% CI: 0%\u20137%) of the 93 were infected with\n                      <jats:italic>Echinococcus<\/jats:italic>\n                      . The north\u2010western region of Iran showed two out of 30 jackals (6.6%) infected with adult\n                      <jats:italic>Echinococcus<\/jats:italic>\n                      compared to one out of 35 jackals (2.8%) in the northern region. DNA from\n                      <jats:italic>Echinococcus<\/jats:italic>\n                      was detected in these individuals by PCR. Based on PCR\u2010RFLP analysis of the\n                      <jats:italic>ITS1<\/jats:italic>\n                      gene and sequencing of\n                      <jats:italic>COX1<\/jats:italic>\n                      ,\n                      <jats:italic>NADH1<\/jats:italic>\n                      and\n                      <jats:italic>ITS1<\/jats:italic>\n                      gene,\n                      <jats:italic>E. granulosus sensu stricto<\/jats:italic>\n                      genotype was confirmed in the jackals that had been infected.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Evidence shows that\n                      <jats:italic>E. granulosus<\/jats:italic>\n                      occurs in jackals in Iran, with the\n                      <jats:italic>E. granulosus s.s<\/jats:italic>\n                      . genotype being the most common. This parasite has been identified as a zoonotic parasite with a genotype that can be transmitted to livestock and humans. Establishing effective control measures to prevent the spread of echinococcosis and ensure public health is crucial.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.1554","type":"journal-article","created":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T10:01:17Z","timestamp":1723111277000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Morphometrical and molecular identification of\n                    <i>Echinococcus granulosus<\/i>\n                    genotypes in wild canids in north of Iran"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6693-0091","authenticated-orcid":false,"given":"Moein Abolhasani","family":"Darounkola","sequence":"first","affiliation":[{"name":"Department of Pathobiology Faculty of Veterinary Medicine Ferdowsi University of Mashhad  Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6470-2528","authenticated-orcid":false,"given":"Elahe","family":"Ebrahimzadeh","sequence":"additional","affiliation":[{"name":"Department of Pathobiology Faculty of Veterinary Medicine Ferdowsi University of Mashhad  Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4706-4995","authenticated-orcid":false,"given":"Hassan","family":"Borji","sequence":"additional","affiliation":[{"name":"Department of Pathobiology Faculty of Veterinary Medicine Ferdowsi University of Mashhad  Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5570-0404","authenticated-orcid":false,"given":"Mohammadreza","family":"Khoshvaght","sequence":"additional","affiliation":[{"name":"Department of Pathobiology Faculty of Veterinary Medicine Ferdowsi University of Mashhad  Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.37077\/25200860.2022.35.1.06"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1079\/JOH2005279"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X10000234"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2023.106925"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-2170-x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2017.02.001"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001379"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2020.105451"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00290\u201000220"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(92)90109-W"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(93)90065-7"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-022-05197-8"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.12647"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(02)00005-5"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2006.06.024"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050386"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.1.107-135.2004"},{"issue":"3","key":"e_1_2_10_19_1","first-page":"302","article-title":"Morphological and genetic characteristics of the liver hydatid cyst of a donkey with Iran origin","volume":"9","author":"Eslami A.","year":"2014","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.09.029"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.2478\/helm-2019-0023"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001915"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/8859116"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vprsr.2017.03.011"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X10000520"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X1800010X"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.5455\/ijlr.20200418070900"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-021-03131-1"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182019000544"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijppaw.2019.08.005"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-019-3859-z"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1017\/s0031182011001661"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.14469"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2009.02.015"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-53230-7_7"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2014.04.001"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X18000275"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182018000719"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2018.06.016"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4458-5"},{"issue":"1","key":"e_1_2_10_41_1","first-page":"204","article-title":"The Echinococcus canadensis (G7) genome: A key knowledge of parasitic platyhelminth human diseases","volume":"18","author":"Maldonado L. L.","year":"2017","journal-title":"BMC Genomics [Electronic Resource]"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X13000631"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2023.106951"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002249"},{"issue":"2","key":"e_1_2_10_45_1","first-page":"131","article-title":"Cystic echinococcosis: A comprehensive review on life cycle, epidemiology, pathogenesis, clinical spectrum, diagnosis, public health and economic implications, treatment, and control","volume":"6","author":"Pal M.","year":"2022","journal-title":"International Journal of Clinical and Experimental Medicine"},{"issue":"2","key":"e_1_2_10_46_1","first-page":"310","article-title":"Epidemiology and clinical features of hydatid cyst in Northern Iran from 2005 to 2015","volume":"13","author":"Parkoohi P. I.","year":"2018","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2012.07.010"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.07.035"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2020.104575"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-1025-9"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008519"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X23000020"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(84)90079-1"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9060453"},{"issue":"4","key":"e_1_2_10_55_1","first-page":"179","article-title":"Genetic variants of Echinococcus granulosus in Slovakia recorded by random amplification of polymorphic DNA","volume":"35","author":"Turcekov\u00e1 L.","year":"1998","journal-title":"Helminthologia"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2020024"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-022-07649-7"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.1554","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vms3.1554","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.1554","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T13:20:21Z","timestamp":1781875221000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.1554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,8]]},"references-count":56,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1002\/vms3.1554"],"URL":"https:\/\/doi.org\/10.1002\/vms3.1554","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.1554\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.1554\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.1554\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.1554\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.1554\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.1554\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.1554\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.1554\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.1554\/v3\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.1554\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.1554\/v3\/review2","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,8]]},"assertion":[{"value":"2023-10-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1554"}}