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We therefore, evaluated the effects of eight treatments with different levels of soybean lecithin concentration (1, 3 and 5%) and egg yolk (20%) in Tris\u2010citric\u2010fructose or Tris\u2010citric\u2010fructose\u2010mineral salts extender on chilled canine sperm quality during 10\u00a0days of storage. The sperm motility was analysed by computer\u2010assisted sperm analysis (<jats:styled-content style=\"fixed-case\">CASA<\/jats:styled-content>), whereas plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential parameters were determined using a fluorescent staining combination of propidium iodide (<jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>), Hoechst 33342 (H342), fluorescein isothiocyanate\u2010conjugated Pisum sativum agglutinin (<jats:styled-content style=\"fixed-case\">FITC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PSA<\/jats:styled-content>) and 5,5\u2032,6,6\u2032\u2010tetrachloro\u20101,1\u2032,3,3\u2032\u2010tetraethylbenzimidazolyl\u2010carbocyanine iodide (<jats:styled-content style=\"fixed-case\">JC<\/jats:styled-content>\u20101) by confocal laser scanning microscope. The results showed that egg yolk was found to be better than soybean lecithin in Tris\u2010citric\u2010fructose or Tris\u2010citric\u2010fructose\u2010mineral salts extender for maintaining the quality of chilled canine sperm within 10\u00a0days of storage (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05). Although egg yolk in Tris\u2010citric\u2010fructose extender could maintain the motility better than other extenders, egg yolk in Tris\u2010citric\u2010fructose\u2010mineral salts extender was the highest in intact plasma membrane, intact acrosome membrane and high mitochondrial membrane potential (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05). In contrast, the sperm quality of soybean lecithin in Tris\u2010citric\u2010fructose\u2010mineral salts extender was lower than that of soybean lecithin in Tris\u2010citric\u2010fructose extender, and soybean lecithin 1% was greater than soybean lecithin 3% and 5% in plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05). In conclusion, soybean lecithin cannot replace egg yolk in Tris\u2010citric\u2010fructose or Tris\u2010citric\u2010fructose\u2010mineral salts extenders, and egg yolk in Tris\u2010citric\u2010fructose\u2010mineral salts extender is superior to other extenders in chilling canine sperm.<\/jats:p>","DOI":"10.1002\/vms3.158","type":"journal-article","created":{"date-parts":[[2019,3,8]],"date-time":"2019-03-08T08:51:29Z","timestamp":1552035089000},"page":"345-360","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Effects of egg yolk and soybean lecithin on sperm quality determined by computer\u2010assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm"],"prefix":"10.1002","volume":"5","author":[{"given":"Vui V.","family":"Nguyen","sequence":"first","affiliation":[{"name":"School of Animal Technology and Innovation Institute of Agricultural Technology Suranaree University of Technology 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