{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T04:54:01Z","timestamp":1788670441025,"version":"build-2803163510"},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T00:00:00Z","timestamp":1562112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005624","name":"Medizinischen Hochschule Hannover","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005624","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["643476"],"award-info":[{"award-number":["643476"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Background: Equine hepacivirus (Eq<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>) in equids represents the closest homologue to hepatitis C virus (<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>) infecting humans. A majority of<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>infected patients develop a chronic course of infection leading to liver fibrosis, cirrhosis and liver failure. However, in horses mostly transient mild subclinical infections are reported for Eq<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>to date. Objectives: Eq<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>can be involved in chronic liver diseases of horses. Methods: Biochemical parameters in serum samples were measured. Viral load was determined using<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>. Next generation sequencing (<jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>) of serum was performed. Liver tissue was stained with haematoxylin and eosin and analysed for viral<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>with fluorescent<jats:italic>in situ<\/jats:italic>\u2010hybridization. Results: The horse showed symptoms of severe hepatopathy and was chronically infected with Eq<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>. Viral<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>was detectable in the liver during disease. To rule out other infectious agents<jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>was performed and showed the highest abundance for Eq<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>. The identified virus sequence was similar to other circulating equine hepaciviruses. Conclusions: Eq<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>can be associated with liver disease in horses. Whether it causes the disease or contributes in a multifactorial manner needs further investigation.<\/jats:p>","DOI":"10.1002\/vms3.181","type":"journal-article","created":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T09:01:09Z","timestamp":1562144469000},"page":"372-378","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3875-0707","authenticated-orcid":false,"given":"Birthe","family":"Tegtmeyer","sequence":"first","affiliation":[{"name":"Institute for Experimental Virology TWINCORE Centre for Experimental and Clinical Infection Research Medical School Hannover (MHH) Helmholtz Centre for Infection Research (HZI) Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Echelmeyer","sequence":"additional","affiliation":[{"name":"Clinic for Horses University of Veterinary Medicine Hannover, Foundation Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa M.","family":"Pfankuche","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Veterinary Medicine Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Puff","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Veterinary Medicine Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Todt","sequence":"additional","affiliation":[{"name":"Institute for Experimental Virology TWINCORE Centre for Experimental and Clinical Infection Research Medical School Hannover (MHH) Helmholtz Centre for Infection Research (HZI) Hannover Germany"},{"name":"Department of Molecular and Medical Virology Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Fischer","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Virology and Hygiene University Medical Centre Hamburg\u2010Eppendorf Hamburg Germany"},{"name":"German Center for Infection Research Partner Site Hamburg\u2010Borstel\u2010L\u00fcbeck\u2010Riems Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy","family":"Durham","sequence":"additional","affiliation":[{"name":"Liphook Equine Hospital Liphook United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karsten","family":"Feige","sequence":"additional","affiliation":[{"name":"Clinic for Horses University of Veterinary Medicine Hannover, Foundation Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Baumg\u00e4rtner","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Veterinary Medicine Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eike","family":"Steinmann","sequence":"additional","affiliation":[{"name":"Institute for Experimental Virology TWINCORE Centre for Experimental and Clinical Infection Research Medical School Hannover (MHH) Helmholtz Centre for Infection Research (HZI) Hannover Germany"},{"name":"Department of Molecular and Medical Virology Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessika\u2010M. 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