{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T20:30:08Z","timestamp":1785529808681,"version":"3.56.0"},"reference-count":59,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,8,14]],"date-time":"2019-08-14T00:00:00Z","timestamp":1565740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005680","name":"Gauteng Department of Agriculture and Rural Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005680","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Brucellosis is an infectious and contagious zoonotic bacterial disease of both humans and animals. In developing countries where brucellosis is endemic, baseline data on the prevalence of brucellosis, using abattoir facilities, is important.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was to determine the seroprevalence of antibodies against <jats:italic>Brucella<\/jats:italic> in slaughter cattle at Gauteng province, South Africa and to characterize isolates of <jats:italic>Brucella<\/jats:italic> spp.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this cross\u2010sectional study, un\u2010clotted blood samples with corresponding organ tissue samples were collected from slaughtered cattle. Serological [Rose Bengal test (<jats:styled-content style=\"fixed-case\">RBT<\/jats:styled-content>), complement fixation test (<jats:styled-content style=\"fixed-case\">CFT<\/jats:styled-content>) and indirect <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">iELISA<\/jats:styled-content>)], molecular (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) and bacteriological methods were used to detect <jats:italic>Brucella<\/jats:italic> antibodies and <jats:italic>Brucella<\/jats:italic> spp. from 200 slaughtered cattle in 14 abattoirs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">RBT<\/jats:styled-content> revealed a seroprevalence of brucellosis as 11.0% (22 of 200) and <jats:styled-content style=\"fixed-case\">iELISA<\/jats:styled-content> confirmed 5.5% (11 of 200). The estimated seroprevalence from <jats:styled-content style=\"fixed-case\">RBT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">iELISA<\/jats:styled-content> was 5.5% while <jats:styled-content style=\"fixed-case\">RBT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CFT<\/jats:styled-content> was 2.0% (4 of 200). <jats:italic>Brucella melitensis<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u00a06) and <jats:italic>B. abortus<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u00a05) were isolated from 11 cattle tissues (5.5%) as confirmed to species level with <jats:styled-content style=\"fixed-case\">AMOS PCR<\/jats:styled-content> and differentiated from vaccine strains with Bruce\u2010ladder <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Seven of the 11 isolates originated from seropositive cattle of which five were biotyped as <jats:italic>B<\/jats:italic>. <jats:italic>abortus<\/jats:italic> bv 1 (<jats:italic>n<\/jats:italic>\u00a0=\u00a02) and <jats:italic>B. melitensis bv<\/jats:italic> 2 (<jats:italic>n<\/jats:italic>\u00a0=\u00a01) and <jats:italic>B. melitensis<\/jats:italic> bv 3 (<jats:italic>n<\/jats:italic>\u00a0=\u00a02).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This is the first documentation of <jats:italic>B. melitensis<\/jats:italic> in cattle in South Africa. The zoonotic risk of brucellosis posed by <jats:italic>Brucella<\/jats:italic>\u2010infected slaughter cattle to abattoir workers and consumers of improperly cooked beef cannot be ignored.<\/jats:p><\/jats:sec>","DOI":"10.1002\/vms3.190","type":"journal-article","created":{"date-parts":[[2019,8,15]],"date-time":"2019-08-15T02:29:48Z","timestamp":1565836188000},"page":"545-555","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Seroprevalence and characterization of <i>Brucella<\/i> species in cattle slaughtered at Gauteng abattoirs, South Africa"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2872-8864","authenticated-orcid":false,"given":"Francis B.","family":"Kolo","sequence":"first","affiliation":[{"name":"Department of Veterinary Tropical Diseases University of Pretoria Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9470-9421","authenticated-orcid":false,"given":"Abiodun A.","family":"Adesiyun","sequence":"additional","affiliation":[{"name":"Department of Production Animal Studies University of Pretoria Pretoria South Africa"},{"name":"Department of Basic Veterinary Sciences Faculty of Medical Sciences University of the West Indies St. Augustine Trinidad and Tobago"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Folorunso O.","family":"Fasina","sequence":"additional","affiliation":[{"name":"Department of Veterinary Tropical Diseases University of Pretoria Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles T.","family":"Katsande","sequence":"additional","affiliation":[{"name":"Gauteng Department of Agriculture and Rural Development Johannesburg South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Banenat B.","family":"Dogonyaro","sequence":"additional","affiliation":[{"name":"Department of Veterinary Tropical Diseases University of Pretoria Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Potts","sequence":"additional","affiliation":[{"name":"Agricultural Research Council - Onderstepoort Veterinary Research Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Itumeleng","family":"Matle","sequence":"additional","affiliation":[{"name":"Agricultural Research Council - Onderstepoort Veterinary Research Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6368-5279","authenticated-orcid":false,"given":"Awoke K.","family":"Gelaw","sequence":"additional","affiliation":[{"name":"Agricultural Research Council - Onderstepoort Veterinary Research Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3577-1273","authenticated-orcid":false,"given":"Henriette","family":"van Heerden","sequence":"additional","affiliation":[{"name":"Department of Veterinary Tropical Diseases University of Pretoria Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,14]]},"reference":[{"issue":"1","key":"e_1_2_12_2_1","first-page":"28","article-title":"Seroprevalence of brucellosis Q\u2010fever and toxoplasmosis in slaughter livestock in Trinidad","volume":"49","author":"Adesiyun A. 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