{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:57:30Z","timestamp":1786978650454,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T00:00:00Z","timestamp":1566777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Brucellosis is a zoonotic disease caused by bacteria\n                      <jats:italic>Brucella<\/jats:italic>\n                      spp. belonging to the genus\n                      <jats:italic>Brucella<\/jats:italic>\n                      . It is endemic in domesticated animals in Bangladesh. Isolation, identification and genetic characterization of\n                      <jats:italic>Brucella<\/jats:italic>\n                      spp. in dairy cattle are essential to undertake appropriate control and preventive measures. The study was conducted to isolate and characterize the\n                      <jats:italic>Brucella<\/jats:italic>\n                      spp. circulating in dairy cattle.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Uterine discharge (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a045), milk (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0115), vaginal swab (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a071), placenta (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a07) and aborted fetus (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a02) were collected. Brucella selective agar plates were inoculated with samples and incubated at 37\n                      <jats:sup>\u25e6<\/jats:sup>\n                      C for 14\u00a0days under 5% CO\n                      <jats:sub>2<\/jats:sub>\n                      for isolation of\n                      <jats:italic>Brucella<\/jats:italic>\n                      spp.\n                      <jats:italic>Brucella<\/jats:italic>\n                      suspected colonies were recovered from samples were confirmed by genus and species specific PCR assays. Genetic characterization was performed by Multi Locus Variable number tandem\u2010repeat Analysis\u201016 (MLVA\u201016).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The isolates of\n                      <jats:italic>Brucella<\/jats:italic>\n                      recovered from samples were confirmed as\n                      <jats:italic>B. abortus<\/jats:italic>\n                      by AMOS\u2010ERY PCR assay. The classical biotyping method confirmed all 10\n                      <jats:italic>B. abortus<\/jats:italic>\n                      isolates belonged to the biovar 3. The MLVA\u201016 assay indicated all\n                      <jats:italic>B. abortus<\/jats:italic>\n                      isolates identical and the same genotype 40, based on panel 1 MLVA\u20108.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Dendrogram analysis revealed all\n                      <jats:italic>B. abortus<\/jats:italic>\n                      isolates of the study were identical to three isolates from Brazil, one isolate of France and closely related to Chinese isolates. This is the first report of isolation and genetic characterization of\n                      <jats:italic>B. abortus<\/jats:italic>\n                      from the dairy cattle in Bangladesh.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.193","type":"journal-article","created":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T23:04:20Z","timestamp":1566860660000},"page":"556-562","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["First isolation, identification and genetic characterization of\n                    <i>Brucella abortus<\/i>\n                    biovar 3 from dairy cattle in Bangladesh"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8683-3251","authenticated-orcid":false,"given":"Md. Sadequl","family":"Islam","sequence":"first","affiliation":[{"name":"Department of Microbiology and Hygiene Bangladesh Agricultural University Mymensingh Bangladesh"},{"name":"Hajee Mohammad Danesh Science and Technology University Dinajpur Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuliano","family":"Garofolo","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise \"G. Caporale\" Teramo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6939-1378","authenticated-orcid":false,"given":"Lorena","family":"Sacchini","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise \"G. Caporale\" Teramo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda C.","family":"Dainty","sequence":"additional","affiliation":[{"name":"Animal and Plant Health Agency Weybridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5561-3643","authenticated-orcid":false,"given":"Mst. Minara","family":"Khatun","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukumar","family":"Saha","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4873-9368","authenticated-orcid":false,"given":"Md. Ariful","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2006.12.015"},{"key":"e_1_2_8_3_1","volume-title":"Techniques for the brucellosis laboratory.","author":"Alton G. G.","year":"1988"},{"key":"e_1_2_8_4_1","unstructured":"Alton G. G. Jones L. M. &Pietz D. E.(1975).Laboratory techniques in brucellosis. Geneva Monograph. 55 113."},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1128\/jcm.32.11.2660-2666.1994","article-title":"Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR","volume":"32","author":"Bricker B. 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