{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T19:53:54Z","timestamp":1779047634325,"version":"3.51.4"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,10,24]],"date-time":"2019-10-24T00:00:00Z","timestamp":1571875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Vice-Chancellor of Research of Shiraz University of Medical Sciences","award":["10436"],"award-info":[{"award-number":["10436"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Balantidium coli<\/jats:italic> is a common parasite of pig and wild boars (<jats:italic>Sus scrofa<\/jats:italic>) which can infect humans and several species of mammals. This study aimed to determine the genotype of <jats:italic>Balantidium<\/jats:italic> isolated from Eurasian wild boars in Bushehr province, Southwestern Iran. Twenty\u2010five faecal samples, originating from 25 wild boars captivated in our previous study, were processed. DNA was extracted from the faecal samples and PCR\u2010amplified, targeting an ITS1\u20135.8s\u2010rRNA\u2013ITS2 region of <jats:italic>Balantidium<\/jats:italic> genome. PCR product was purified from the gel, and sequenced. BLAST analysis was performed in order to compare our isolates with other previously reported ones. A phylogenetic tree was constructed, using MegaX software, to find out the phylogenetic diversity of the isolates. With PCR it was possible to detect <jats:italic>Balantidium<\/jats:italic> DNA in the faecal samples of 13 out of 25 (52%) of the wild boars. BLAST analysis of seven isolates revealed that the isolates belong to the newly introduced genus <jats:italic>Neobalantidium coli<\/jats:italic>. Sequences of three isolates were deposited in the GenBank. Moreover, molecular analysis revealed six areas of nucleotide differences within the isolates and nine areas of difference between the sequences obtained in this study and those available in the GenBank. Phylogenetic analysis revealed that the sequences of isolates of this study have up to 2.2% dissimilarity from those published in the GenBank. The findings of this study, for the first time, revealed that some of the isolates of <jats:italic>Balantidium<\/jats:italic> originating from wild boars in Southwestern Iran belonged to the <jats:italic>N. coli.<\/jats:italic><\/jats:p>","DOI":"10.1002\/vms3.210","type":"journal-article","created":{"date-parts":[[2019,10,25]],"date-time":"2019-10-25T06:55:28Z","timestamp":1571986528000},"page":"142-146","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<i>Neobalantidium coli<\/i>: First molecular identification from the Eurasian wild boar, <i>Sus Scrofa<\/i> in Bushehr Province, Southwestern Iran"],"prefix":"10.1002","volume":"6","author":[{"given":"Shamsi","family":"Noorpisheh Ghadimi","sequence":"first","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz Iran"},{"name":"Department of Parasitology and Mycology Fasa University of Medical Sciences Fasa Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad R.","family":"Abedini","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2045-9057","authenticated-orcid":false,"given":"Bahador","family":"Sarkari","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz Iran"},{"name":"Basic Sciences in Infectious Diseases Research Center Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amir","family":"Savardashtaki","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology School of Advanced Medical Sciences and Technologies Shiraz University of Medical Sciences Shiraz 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