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This study aimed to investigate the antimicrobial activity of yeast glycoprotein (YG) against\n                    <jats:italic>Aeromonas caviae<\/jats:italic>\n                    . Pathogens were isolated from liver of diseased\n                    <jats:italic>Carassius auratus gibelio<\/jats:italic>\n                    . Based on morphological and biochemical analysis, together with 16S rRNA gene sequencing, the isolated strains were identified as\n                    <jats:italic>A. caviae<\/jats:italic>\n                    and concluded as clones of a single strain and named L2. Further pathogenicity analysis revealed that\n                    <jats:italic>A. caviae<\/jats:italic>\n                    possessed \u03b2\u2010haemolysis, and its median lethal dose for\n                    <jats:italic>C. gibelio<\/jats:italic>\n                    was 1.33\u00a0\u00d7\u00a010\n                    <jats:sup>6<\/jats:sup>\n                    \u00a0CFU\/ml. Hepatic adenylate kinase and pyruvate kinase activities of\n                    <jats:italic>C. gibelio<\/jats:italic>\n                    were inhibited post\u2013\n                    <jats:italic>A. caviae<\/jats:italic>\n                    infection. Antimicrobial drug test suggested that\n                    <jats:italic>A. caviae<\/jats:italic>\n                    was a multidrug\u2010resistant organism but could be inhibited by YG in vitro. Minimum inhibitory and bactericidal concentration of YG was 83.3\u00a0mg\/ml and 166.7\u00a0mg\/ml, respectively. Microbiota sequencing results showed that YG supplement could obviously decrease the relative abundance of\n                    <jats:italic>Aeromonas<\/jats:italic>\n                    and increase the microbial diversity. Our study revealed that\n                    <jats:italic>A. caviae<\/jats:italic>\n                    from\n                    <jats:italic>C. gibelio<\/jats:italic>\n                    was a multidrug\u2010resistant bacteria strain, and could be significantly inhibited by YG in vivo and in vitro, thus providing important insights into ecological control and pathogenesis of\n                    <jats:italic>A. caviae<\/jats:italic>\n                    in aquaculture.\n                  <\/jats:p>","DOI":"10.1002\/vms3.253","type":"journal-article","created":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T08:55:26Z","timestamp":1585040126000},"page":"639-648","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A potential alternative to traditional antibiotics in aquaculture: Yeast glycoprotein exhibits antimicrobial effect in vivo and in vitro on\n                    <i>Aeromonas caviae<\/i>\n                    isolated from\n                    <i>Carassius auratus gibelio<\/i>"],"prefix":"10.1002","volume":"6","author":[{"given":"Ronghua","family":"Wu","sequence":"first","affiliation":[{"name":"Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education Southwest University Chongqing China"},{"name":"College of Animal Science and Technology Institute of Three Gorges Ecological Fisheries of Chongqing Southwest University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junyu","family":"Shen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education Southwest University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dandan","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Institute of Three Gorges Ecological Fisheries of Chongqing Southwest University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaqian","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Institute of Three Gorges Ecological Fisheries of Chongqing Southwest University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7528-6750","authenticated-orcid":false,"given":"Tao","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education Southwest University Chongqing China"},{"name":"College of Animal Science and Technology Institute of Three Gorges Ecological Fisheries of Chongqing Southwest University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhua","family":"Yi","sequence":"additional","affiliation":[{"name":"Angel Yeast Co., Ltd Yichang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6290-9522","authenticated-orcid":false,"given":"Yun","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education Southwest University Chongqing China"},{"name":"College of Animal Science and Technology Institute of Three Gorges Ecological Fisheries of Chongqing Southwest University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,3,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1516-89132012000200001"},{"issue":"39","key":"e_1_2_9_3_1","first-page":"7658","article-title":"Antimicrobial potentials of Eclipta alba by disc diffusion method","volume":"10","author":"Bakht J.","year":"2011","journal-title":"African Journal of Biotechnology"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12746"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.3019"},{"key":"e_1_2_9_6_1","volume-title":"Bergey's manual of determinative bacteriology","author":"Buchanan R. 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