{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:13:00Z","timestamp":1772154780974,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T00:00:00Z","timestamp":1586217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In this study, Multiple Locus Variable Number Tandem Repeat Analysis (MLVA\u201016) was performed on 18\u00a0\n                    <jats:italic>Brucella<\/jats:italic>\n                    isolates identified\n                    <jats:italic>\u00a0<\/jats:italic>\n                    bacteriologically and molecularly (AMOS\u2010PCR) as\u00a0\n                    <jats:italic>Brucella abortus<\/jats:italic>\n                    \u00a0(\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a06) and\u00a0\n                    <jats:italic>Brucella melitensis<\/jats:italic>\n                    \u00a0(\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a012). This was aimed to study the genetic association among some Egyptian\u00a0\n                    <jats:italic>Brucella\u00a0<\/jats:italic>\n                    genotypes isolated during the period from 2002 to 2013 along with the global genotypes database. MLVA\u201016 analysis for\u00a0\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    and\u00a0\n                    <jats:italic>B. abortus\u00a0<\/jats:italic>\n                    strains illustrates a total of 11, and 3 genotypes with 10 and 1 singleton genotypes, respectively.\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    strains displayed greater markers diversity by VNTRs analysis of the 16 loci than\u00a0\n                    <jats:italic>B. abortus<\/jats:italic>\n                    and this was attributed mainly to the diverging in panel 2B markers.\u00a0\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    \u00a0genotype M4_Fayoum_Giza (3,5,3,13,1,1,3,3,8,21,8,7,5,9,5,3) was the only predominated genotype circulating between two different governorates. The most common\n                    <jats:italic>B. abortus<\/jats:italic>\n                    genotype, GT A3_Dakahlia (4,5,4,12,2,2,3,3,6,21,8,4,4,3,4,4), was present in three identical isolates. In phylogeny, Egyptian\u00a0\n                    <jats:italic>B. abortus<\/jats:italic>\n                    \u00a0bv1 genotypes were closely related to East Asian strain (for the first time), Western Mediterranean and Americas clonal lineages.\u00a0\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    \u00a0local genotypes exhibit a genetic relatedness mostly to Western Mediterranean clonal lineage and one strain of Eastern Mediterranean clonal lineage. In conclusion, the geographic location is not the only factor stands behind the high genetic similarity of the Egyptian\u00a0\n                    <jats:italic>Brucella<\/jats:italic>\n                    \u00a0genotypes. These low variations may be a result of a stepwise mutational event of the most variable loci from a very limited number of ancestors especially during the transmission through non\u2010preference hosts. The authors encourage the authorities in charge to establish pre\u2010movement testing to reduce the risk of brucellosis spread.\n                  <\/jats:p>","DOI":"10.1002\/vms3.260","type":"journal-article","created":{"date-parts":[[2020,4,8]],"date-time":"2020-04-08T01:04:11Z","timestamp":1586307851000},"page":"421-432","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Genetic variation of\n                    <i>Brucella<\/i>\n                    isolates at strain level in Egypt"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5554-7984","authenticated-orcid":false,"given":"Nour H.","family":"Abdel\u2010Hamid","sequence":"first","affiliation":[{"name":"Department of Brucellosis Research Animal Health Research Institute Agricultural Research Center Giza Dokki Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Essam M.","family":"El\u2010bauomy","sequence":"additional","affiliation":[{"name":"Department of Brucellosis Research Animal Health Research Institute Agricultural Research Center Giza Dokki Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hazem M.","family":"Ghobashy","sequence":"additional","affiliation":[{"name":"Department of Brucellosis Research Animal Health Research Institute Agricultural Research Center Giza Dokki Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abeer A.","family":"Shehata","sequence":"additional","affiliation":[{"name":"Fayoum Regional Laboratory Animal Health Research Institute Agricultural Research Center Fayoum Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4,7]]},"reference":[{"issue":"4","key":"e_1_2_9_2_1","first-page":"11","article-title":"Bacteriological examination and diagnostic performance of some serological tests used in diagnosis of small ruminant brucellosis","volume":"26","author":"Abdel\u2010Hamid N. 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