{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T21:52:55Z","timestamp":1777672375177,"version":"3.51.4"},"reference-count":71,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,4,3]],"date-time":"2020-04-03T00:00:00Z","timestamp":1585872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Dschang of Cameroon"},{"name":"Molecular Parasitology and Entomology"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Brucellosis is one of the world's most widespread bacterial zoonoses caused by\n                    <jats:italic>Brucella.<\/jats:italic>\n                    It leads to considerable economic losses as a result of low productivity of infected animals and the long debilitating illness in humans. Despite its impact on human and animal health, little attention has been paid on\n                    <jats:italic>Brucella<\/jats:italic>\n                    infections in domestic animals. It is in this light that the prevalence of\n                    <jats:italic>Brucella<\/jats:italic>\n                    antibodies was determined in domestic animals with the overarching goal of improving our knowledge on brucellosis in southern Cameroon. During cross\u2010sectional studies conducted from December 2016 to August 2018 in five sites of southern Cameroon, blood samples were collected in cattle, sheep, goat, pig and dog. Plasma was obtained from each blood sample and\n                    <jats:italic>Brucella<\/jats:italic>\n                    antibodies were detected using the Rose Bengal test and the enzyme\u2010linked immunosorbent assay (ELISA). From 1873 animals that were sampled, the overall prevalence of\n                    <jats:italic>Brucella<\/jats:italic>\n                    antibodies using Indirect enzyme\u2010linked immunosorbent assay (i\u2010ELISA) was 6.35% (118\/1873): 9.12% (78\/855) in cattle; 8.04% (30\/373) in sheep; 6.06% (2\/33) in dog, 1.87% (3\/160) in pig and 1.1% (5\/452) in goat. Between animal species (\n                    <jats:italic>p<\/jats:italic>\n                    \u2010value\u00a0&lt;\u00a0.0001,\n                    <jats:italic>x<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u00a0=\u00a033.63) as well as sampling sites (\n                    <jats:italic>p<\/jats:italic>\n                    \u2010value\u00a0=\u00a0.0001,\n                    <jats:italic>x<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u00a0=\u00a018.97), significant differences were observed in the prevalence of\n                    <jats:italic>Brucella<\/jats:italic>\n                    antibodies. Yoko and Noun localities have shown the highest prevalence of 8.6% (30\/348) and 7.2% (78\/1070), respectively. This prevalence was significantly higher (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a0.03,\n                    <jats:italic>x<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u00a0=\u00a01.25) in female than male cattle. Between adult (16.923%) and young cattle (7.8%), significant difference (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a0.04,\n                    <jats:italic>x<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u00a0=\u00a06.42) was observed in the prevalence of\n                    <jats:italic>Brucella<\/jats:italic>\n                    antibodies. This study shows that the prevalence of\n                    <jats:italic>Brucella<\/jats:italic>\n                    antibodies varies between animal species and localities. It also shows several domestic animals of southern Cameroon that have been in contact with\n                    <jats:italic>Brucella<\/jats:italic>\n                    . It enabled to identify villages where investigations on the transmission dynamic must be focused for the final goal of developing control measures for this neglected zoonotic disease.\n                  <\/jats:p>","DOI":"10.1002\/vms3.264","type":"journal-article","created":{"date-parts":[[2020,4,3]],"date-time":"2020-04-03T11:32:07Z","timestamp":1585913527000},"page":"410-420","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Detection of\n                    <i>Brucella<\/i>\n                    antibodies in domestic animals of southern Cameroon: Implications for the control of brucellosis"],"prefix":"10.1002","volume":"6","author":[{"given":"Rolin M. N.","family":"Kamga","sequence":"first","affiliation":[{"name":"Molecular Parasitology and Entomology Unit Department of Biochemistry Faculty of Science University of Dschang Dschang Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barberine A.","family":"Silatsa","sequence":"additional","affiliation":[{"name":"Molecular Parasitology and Entomology Unit Department of Biochemistry Faculty of Science University of Dschang Dschang Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oumarou","family":"Farikou","sequence":"additional","affiliation":[{"name":"Special Mission for Eradication of Tsetse flies Regional tsetse Division of Adamawa MINEPIA Ngaoundere Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jules\u2010Roger","family":"Kuiate","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Science University of Dschang Dschang Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0449-818X","authenticated-orcid":false,"given":"Gustave","family":"Simo","sequence":"additional","affiliation":[{"name":"Molecular Parasitology and Entomology Unit Department of Biochemistry Faculty of Science University of Dschang Dschang Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4,3]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclepro.2015.02.094"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.5.6.749-754.1998"},{"key":"e_1_2_12_4_1","unstructured":"Alton G. G. Jones L. M. Angus R. D. &Verger J. M.(1988).Technics for the brucellosis laboratory Institut National de la Recherche Agronomique Paris.https:\/\/doi.org\/10.12691\/ajidm\u20104\u20105\u20101"},{"key":"e_1_2_12_5_1","first-page":"125","article-title":"Brucellosis: An update: The perspective from the Mediterranean basin","volume":"10","author":"Ariza J.","year":"1999","journal-title":"Review of Medical Microbiology"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040317"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40064-016-2547-0"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-015-0504-8"},{"key":"e_1_2_12_9_1","first-page":"1","article-title":"Foetal maceration associated with Brucella ovis infection in a Yankassa ewe","volume":"12","author":"Ate I. U.","year":"2011","journal-title":"Revista Electr\u00f3nica De Veterinaria"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/3468596"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-018-3522-x"},{"key":"e_1_2_12_12_1","unstructured":"Ayayi J. A. T\u00eako\u2010Agbo A. &Kon\u00e9 P.(2009).The impact of brucellosis on the economy and public health in Africa Inter\u2010State School of Veterinary Sciences and Medicine1 Conf. OIE (pp.85\u201398)."},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-015-0535-1"},{"issue":"2","key":"e_1_2_12_14_1","first-page":"38","article-title":"Bovine brucellosis in cattle production systems in the Western Highlands of Cameroon","volume":"1","author":"Bayemi P. H.","year":"2015","journal-title":"International Journal of Animal Biology"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-008-9184-8"},{"issue":"19","key":"e_1_2_12_16_1","first-page":"25","article-title":"Ovine and caprine brucellosis: World distribution and control\/eradication strategies in West Asia\/North Africa region","volume":"62","author":"Benkirane A.","year":"2006","journal-title":"Small Ruminant Research"},{"key":"e_1_2_12_17_1","first-page":"65","article-title":"Seroepidemiological investigation of bovine brucellosis in the extensive cattle production system of Tigray region of Ethiopia","volume":"5","author":"Berhe G.","year":"2007","journal-title":"The International Journal of Applied Research in Veterinary Medicine"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2015.08.014"},{"key":"e_1_2_12_19_1","first-page":"163","article-title":"Serological survey of brucellosis in livestock animals and workers in Ibadan, Nigeria","volume":"9","author":"Cadmus S. I. B.","year":"2006","journal-title":"African Journal of Biomedical Research"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.89.21.552"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0302.970219"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2018.06.001"},{"key":"e_1_2_12_23_1","first-page":"173","article-title":"Epididymitis of rams in the central and southern districts of the Orange Free State","volume":"55","author":"De Wet J. A.","year":"1984","journal-title":"Journal of the South African Veterinary Association"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001865"},{"issue":"5","key":"e_1_2_12_25_1","first-page":"508","article-title":"Sero\u2010prevalence study of bovine brucellosis in Pastoral and Agro\u2010Pastoral Areas of East Showa Zone, Oromia Regional State, Ethiopia","volume":"6","author":"Dinka H.","year":"2009","journal-title":"American Eurasian Journal Agricultural Environmental Sciences"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2015.10.023"},{"issue":"2","key":"e_1_2_12_27_1","first-page":"62","article-title":"Sero\u2010prevalence of Brucella abortus in buffaloes and wildebeests in the Serengeti ecosystem: A threat to humans and domestic ruminants","volume":"26","author":"Fyumagwa R. D.","year":"2009","journal-title":"Tanzania Veterinary Journal"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.157.21.668"},{"key":"e_1_2_12_29_1","first-page":"454","article-title":"Sero\u2010prevalence of brucellosis in food animals in the Punjab, Pakistan","volume":"34","author":"Gul S. T.","year":"2014","journal-title":"Pakistan Veterinary Journal"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001923"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268896007005"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1863-2378.2011.01425.x"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146538"},{"key":"e_1_2_12_34_1","first-page":"1","article-title":"Sero\u2010prevalence of anti\u2010brucella antibodies in goats in El\u2010Gedarif state, Eastern Sudan","volume":"4","author":"Khuzaima B. A.","year":"2018","journal-title":"Journal of Animal Veterinary Science"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.24248\/EAHRJ-D-16-00379"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.160.11.362"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006456"},{"key":"e_1_2_12_38_1","volume-title":"Bovine brucellosis in Sub\u2010Saharan Africa: Estimation of sero\u2010prevalence and impact on meat and milk offtake potential","author":"Mangen M. J.","year":"2002"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-012-0268-0"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-010-9748-2"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-53-24"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2009.12.001"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0094168"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2018.511-518"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-018-1515-z"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2007.03.003"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-011-9886-1"},{"issue":"1","key":"e_1_2_12_48_1","first-page":"65","article-title":"Serological diagnosis of brucellosis","volume":"31","author":"Nielsen K.","year":"2010","journal-title":"Prilozi"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2011182171"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2010171061"},{"issue":"6","key":"e_1_2_12_51_1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.54846\/jshap\/640","article-title":"Production characteristics, disease prevalence, and herdhealth management of pigs in Southeast Nigeria","volume":"19","author":"Nwanta J. A.","year":"2011","journal-title":"Journal of Swine Health and Production"},{"key":"e_1_2_12_52_1","unstructured":"OIE. (2013a).Bovine brucellosis. In Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (pp.1\u201335)."},{"key":"e_1_2_12_53_1","unstructured":"OIE. (2013b).Caprine and ovine brucellosis (excluding Brucella ovis). In Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (pp.1\u201310)."},{"key":"e_1_2_12_54_1","first-page":"135","article-title":"An investigation of abortion in sheep in Rano L.I.B.C. near Kano, Nigeria","volume":"28","author":"Okoh A. E.","year":"1980","journal-title":"Bulletin of Animal Health and Production in Africa"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.4314\/nvj.v32i1.68989"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2012.11.029"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.17221\/1555-VETMED"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00248-1"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2012.05.010"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008623"},{"key":"e_1_2_12_61_1","first-page":"1","volume-title":"ID Screen Brucellosis Serum Indirect Multi\u2010species factsheet","author":"ID\u2010VET","year":"2008"},{"key":"e_1_2_12_62_1","unstructured":"Seignobos C.(2008).La Question mbororo. R\u00e9fugi\u00e9s de la RCA au Cameroun Yaound\u00e9\/Paris (HCR\/SCAC\/IRD)."},{"issue":"3","key":"e_1_2_12_63_1","first-page":"125","article-title":"Sero\u2010prevalence of bovine brucellosis using indirect ELISA in Quetta Balochistan, Pakistan","volume":"22","author":"Shafee M.","year":"2012","journal-title":"Journal of Animal and Plant Science Plant"},{"issue":"7239156","key":"e_1_2_12_64_1","first-page":"12","article-title":"Comparative seroepidemiological study of brucellosis in sheep under smallholder farming and governmental breeding ranches of Central and North East Ethiopia","volume":"2018","author":"Shimeles A. A.","year":"2018","journal-title":"Journal of Veterinary Medicine Article"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1510982112"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2019.02.002"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2007.05.024"},{"key":"e_1_2_12_68_1","first-page":"191","volume-title":"The nature of brucellosis","author":"Spink W. W.","year":"1956"},{"issue":"34","key":"e_1_2_12_69_1","first-page":"229","article-title":"Antibodies to some swine disease in commercial piggeries in Central Zambia","volume":"45","author":"Stafford K.","year":"1992","journal-title":"Revue D\u2018\u00e9levage Et De M\u00e9decine V\u00e9t\u00e9rinaire Des Pays Tropicaux"},{"key":"e_1_2_12_70_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005006"},{"key":"e_1_2_12_71_1","first-page":"610","volume-title":"Veterinary epidemiology","author":"Thrusfield M.","year":"2007"},{"key":"e_1_2_12_72_1","first-page":"223","article-title":"Infectious infertility in sheep","volume":"29","author":"Van Rensburg S. W.","year":"1958","journal-title":"Journal of the South African Veterinary Association"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fvms3.264","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.264","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vms3.264","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.264","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T07:27:24Z","timestamp":1722670044000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.264"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,3]]},"references-count":71,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["10.1002\/vms3.264"],"URL":"https:\/\/doi.org\/10.1002\/vms3.264","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.264\/v4\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.264\/v3\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.264\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.264\/v3\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.264\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.264\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.264\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.264\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.264\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.264\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.264\/v4\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.264\/v4\/review1","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,3]]},"assertion":[{"value":"2019-09-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}