{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:13:22Z","timestamp":1772118802075,"version":"3.50.1"},"reference-count":60,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T00:00:00Z","timestamp":1587427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["420110\/2018\u20106"],"award-info":[{"award-number":["420110\/2018\u20106"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Although Brazil has one of the largest buffalo populations in the Americas, buffalo leptospirosis is still poorly explored when compared to that in bovines; thus, the aim of this research was to carry out a large serological study for leptospirosis in this species in the Brazilian Amazon. For this, we collected 1,405 serum samples from buffaloes raised in the Amazon delta region, which is considered a major area of buffalo production in Brazil. The test used was a microscopic agglutination test (MAT) adopting 34\n                    <jats:italic>Leptospira<\/jats:italic>\n                    antigens, some of which have never been tested for buffaloes in Brazil, including autochthonous strains; in total, 20 serogroups were evaluated. From the total of 1,405 serum samples, 894 (63.6%) reacted in the MAT to at least one of the 20 serogroups, and 511 (36.4%) did not react. The serogroups Sejroe, Autumnalis and Pomona were the most prevalent, with titres ranging from 100 to 12,800, and the autochthonous strains used were not significant in relation to the reference serovars. Leptospirosis in buffaloes seems to have a serological profile similar to leptospirosis in cattle, mainly due to the prevalence of the Sejroe serogroup; however, the results of this study suggested that in the Brazilian Amazon,\n                    <jats:italic>Leptospira<\/jats:italic>\n                    strains that are serologically distinct from the autochthonous strains isolated in the southeastern region of Brazil may be circulating in these animals. Other serovars could also be inserted into the panel of antigens used in MAT for serological studies on buffaloes.\n                  <\/jats:p>","DOI":"10.1002\/vms3.271","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T20:20:30Z","timestamp":1587500430000},"page":"433-440","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Prevalence of\n                    <i>Leptospira<\/i>\n                    serogroups in buffaloes from the Brazilian Amazon"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9843-1452","authenticated-orcid":false,"given":"Israel B.","family":"Guedes","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4604-6034","authenticated-orcid":false,"given":"Gisele O.","family":"de Souza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilian A. R.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1468-658X","authenticated-orcid":false,"given":"Juliana F. de P.","family":"Castro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4728-1228","authenticated-orcid":false,"given":"Ant\u00f4nio F.","family":"de Souza Filho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anderson L. P.","family":"Maia","sequence":"additional","affiliation":[{"name":"M\u00e9dico Veterin\u00e1rio Auditor Fiscal Agropecu\u00e1rio Ag\u00eancia de Defesa e Inspe\u00e7\u00e3o Agropecu\u00e1ria do Estado do Amap\u00e1  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6444-9788","authenticated-orcid":false,"given":"Marcos B.","family":"Heinemann","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4,21]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"77","article-title":"Leptospirosis in water buffalo (Bubalus bubalis) in Trinidad","volume":"79","author":"Adesiyun A. A.","year":"2009","journal-title":"Veterinarski Arhiv"},{"key":"e_1_2_9_3_1","first-page":"98","article-title":"Leptospiral infection in lactating buffaloes","volume":"10","author":"Ahmed R.","year":"1990","journal-title":"Pakistan Veterinary Journal"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1957.6.871"},{"key":"e_1_2_9_5_1","first-page":"15","article-title":"Seroprevalence of leptospirosis among buffaloes from Tamil Nadu","volume":"24","author":"Balakrishman G.","year":"2015","journal-title":"Indian Journal of Veterinary Research"},{"key":"e_1_2_9_6_1","first-page":"87","article-title":"Diagnosis of Bovine Leptospirosis by 16s rRNA Based Polymerase Chain Reaction","volume":"10","author":"Balakrishnan G.","year":"2014","journal-title":"Indian Journal of Field Veterinarians"},{"key":"e_1_2_9_7_1","first-page":"113","article-title":"Caracteriza\u00e7\u00e3o do Turismo de Base Comunit\u00e1ria em Polos Tur\u00edsticos do Estado do Par\u00e1","volume":"10","author":"Barreto E. O.","year":"2017","journal-title":"Revista Brasileira De Ecoturismo"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.4081\/ijas.2007.s2.162"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005228"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-736X2012000700008"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.4238\/gmr.15018473"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2018.840-844"},{"key":"e_1_2_9_13_1","first-page":"5","article-title":"Identification of cattle, buffaloes and rodents as reservoir animals of Leptospira in the District of Gampaha, Sri Lanka","volume":"10","author":"Denipitiya D. T. H.","year":"2017","journal-title":"Biomed Central Research Notes"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1742170511000366"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.15232\/aas.2018-01810"},{"key":"e_1_2_9_16_1","volume-title":"Leptospira and leptospirosis","author":"Faine S.","year":"1999"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-84782002000400011"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1590\/1809-43921998282126"},{"key":"e_1_2_9_19_1","first-page":"5","article-title":"Anticorpos anti\u2010Neospora caninum e contra outros Agentes de Abortamentos em B\u00fafalas da Regi\u00e3o do Vale do Ribeira, S\u00e3o Paulo, Brasil","volume":"68","author":"Fujii T. U.","year":"2001","journal-title":"Arquivos do Instituto Biol\u00f3gico"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2019.01.011"},{"key":"e_1_2_9_21_1","first-page":"341","article-title":"Seroprevalence of Leptospiral Infection in Buffalo (Bubalus bubalis)","volume":"50","author":"Hajikolaei M. R. H.","year":"2006","journal-title":"Bulletin\u2010 Veterinary Institute in Pulawy"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.2004.00740.x"},{"key":"e_1_2_9_23_1","unstructured":"IBGE Instituto Brasileiro de Geografia e Estat\u00edstica. (2017).Censo Agro 2017 Resultados preliminares Bubalinos\/Brasil. Retrieved fromhttps:\/\/censoagro2017.ibge.gov.br\/templates\/censo_agro\/resultadosagro\/pecuaria.html?localidade=0&tema=75659"},{"key":"e_1_2_9_24_1","first-page":"245","article-title":"Studium antigennej structury leptospir. Klasifikacia serologickej skupiny Hebdomadis","volume":"2","author":"Kmety E.","year":"1977","journal-title":"Folia Facultatea Medicina Universitate Comenianae Bratislava"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-013-0427-y"},{"key":"e_1_2_9_26_1","first-page":"457","article-title":"Honden als leptospirendragers","volume":"41","author":"Kouwenaar W.","year":"1929","journal-title":"Nederlandsch Indische Bladen Voor Diergeneeskunde"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-84781999000200019"},{"key":"e_1_2_9_28_1","first-page":"9","article-title":"Atualiza\u00e7\u00e3o em leptospirose bovina","volume":"18","author":"Lilenbaum W.","year":"1996","journal-title":"Revista Brasileira De Medicina Veterin\u00e1ria"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.2008.00305.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.161.9.310"},{"key":"e_1_2_9_31_1","unstructured":"Marvulo M. F. V. Paula C. D. Ferreira P. M. Morais Z. M. Delbem A. C. B. F\u00e1vero A. C. M. \u2026Verdade L. M.(2002).Detection ofLeptospirain two free living populations of capybaras (Hydrochaeris hydrochaeris) from S\u00e3o Paulo State Brazil. International Leptospirosis Society Bridgetown 62."},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1638\/2007-0042.1"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-5150-pvb-4992"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822008000300017"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731113001109"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.15361\/2175-0106.2013v29n4p33"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjm.2018.02.007"},{"key":"e_1_2_9_38_1","first-page":"1512","article-title":"Seroprevalence and predominant serogroups of Leptospira sp. in serological tests of ruminants in northeastern Brazil","volume":"40","author":"Pimenta C. L. R. M.","year":"2019","journal-title":"Semina: Ci\u00eancias Agr\u00e1rias"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-018-1512-z"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)01280-6"},{"issue":"4","key":"e_1_2_9_41_1","article-title":"Detec\u00e7\u00e3o de anticorpo anti\u2010Brucella sp. e anti\u2010 Leptospira spp. em b\u00fafalos (Bubalus bubalis) abatidos em matadouro na cidade de","volume":"62","author":"Rocha K. S.","year":"2019","journal-title":"Bel\u00e9m, Par\u00e1. Revista De Ci\u00eancias Agr\u00e1rias"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-84782007000400046"},{"key":"e_1_2_9_43_1","first-page":"1719","article-title":"A new leptospiral serotype in the Bataviae group, isolated in Sao Paulo, Brasil","volume":"33","author":"Santa Rosa C. A.","year":"1972","journal-title":"American Journal of Veterinary Research"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-736X2012000700003"},{"key":"e_1_2_9_45_1","first-page":"95","article-title":"Seroprevalence of leptospirosis in she\u2010buffaloes (Bos bubalis) at slaughter in Chennai, India","volume":"29","author":"Selvaraj J.","year":"2010","journal-title":"Buffalo Bulletin"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.agsy.2005.02.003"},{"key":"e_1_2_9_47_1","first-page":"12","article-title":"Perfil de bov\u00eddeos abatidos nos matadouros oficiais do Estado do Amap\u00e1, Brasil","volume":"8","author":"Soares B. V.","year":"2014","journal-title":"Publica\u00e7\u00f5es Em Medicina Veterin\u00e1ria E Zootecnia"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268812002981"},{"key":"e_1_2_9_49_1","first-page":"281","article-title":"Leptospirosis: An emerging health problem in Thailand","volume":"36","author":"Tangkanakul W.","year":"2005","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2011.11-0276"},{"key":"e_1_2_9_51_1","first-page":"294","article-title":"Leptospirose Bovina: Aumento na incid\u00eancia da Leptospira interrogans sorovar butembo no rebanho do Estado de Santa Catarina, Brasil","volume":"4","author":"Tonin A. A.","year":"2010","journal-title":"Acta Veterinaria Brasilica"},{"key":"e_1_2_9_52_1","first-page":"319","article-title":"Isolation of Leptospira andaman from an active case of jaundice in a Buffalo","volume":"60","author":"Upadhye A. S.","year":"1983","journal-title":"Indian Veterinary Journal"},{"key":"e_1_2_9_53_1","first-page":"121","article-title":"Buffalo under Threat in Amazon Valley, Brazil","volume":"32","author":"Vale W. G.","year":"2013","journal-title":"Buffalo Bulletin"},{"key":"e_1_2_9_54_1","first-page":"7","article-title":"Leptospirose bovina. N\u00edveis de ocorr\u00eancia e sorotipos predominantes em rebanhos dos Estados de Minas Gerais, S\u00e3o Paulo, Rio de Janeiro, Paran\u00e1, Rio Grande do Sul e Mato Grosso do Sul, no per\u00edodo de janeiro a abril de 1996","volume":"64","author":"Vasconcellos S. A.","year":"1997","journal-title":"Arquivos do Instituto Biol\u00f3gico"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822001000400008"},{"key":"e_1_2_9_56_1","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1590\/1808-1657v76p4532009","article-title":"Ocorr\u00eancia de Anticorpos anti\u2010 Neospora caninum, Brucella sp. e Leptospira spp. em B\u00fafalos (Bubalus bubalis) criados na Amaz\u00f4nia","volume":"76","author":"Viana R. B.","year":"2009","journal-title":"Arquivos do Instituto Biol\u00f3gico"},{"key":"e_1_2_9_57_1","first-page":"15","article-title":"Serological investigation of Leptospira infection and its circulation in one intensive\u2010type water buffalo farm in the Philippines","volume":"64","author":"Villanueva M. A.","year":"2016","journal-title":"Japanese Journal of Veterinary Research"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11625-015-0352-2"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2011.10-0393"},{"key":"e_1_2_9_60_1","first-page":"736","article-title":"Serological Survey of Leptospirosis in Thai Swamp Buffalo (Bubalus bubalis) in Sakon Nakhon Province, Thailand","volume":"46","author":"Wongpanit K.","year":"2012","journal-title":"Kasetsart Journal \u2013 Natural Science"},{"key":"e_1_2_9_61_1","first-page":"25","article-title":"Leptospira biflexa sorotipo ranarum isolado de feto abortado de equino","volume":"17","author":"Yasuda P. H.","year":"1986","journal-title":"Revista De Microbiologia"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fvms3.271","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.271","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vms3.271","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T04:16:59Z","timestamp":1693887419000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,21]]},"references-count":60,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["10.1002\/vms3.271"],"URL":"https:\/\/doi.org\/10.1002\/vms3.271","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.271\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.271\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.271\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.271\/v1\/review4","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.271\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.271\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.271\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.271\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.271\/v2\/response1","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,21]]},"assertion":[{"value":"2019-08-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}