{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T20:33:15Z","timestamp":1793824395549,"version":"4.6.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T00:00:00Z","timestamp":1592956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007925","name":"Huazhong Agricultural University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Porcine respiratory disease complex (PRDC), a respiratory disease caused by a variety of factors, is one of the most common problems in the intensive pig farms. To investigate the mixed infection incidence of wild\u2010type pseudorabies virus (WT PRV) and respiratory bacteria, a total of 1,293 clinical samples were collected from pigs with typical respiratory signs from 14 different provinces of China from September 2016 to February 2018. The WT PRV was detected by ELISA targeting\n                    <jats:italic>gE<\/jats:italic>\n                    antibody while the bacteria were detected by bacterial isolation and serotyping by PCR. The results revealed that the detection rate of\n                    <jats:italic>A. pleuropneumoniae<\/jats:italic>\n                    and\n                    <jats:italic>B. bronchiseptica<\/jats:italic>\n                    infection associated with WT PRV infection were 6.30% and 15.99%, respectively, which were significantly higher than those without WT PRV infection (3.41% and 4.41%) at the farm level (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05). There were no significant differences in the detection rate of\n                    <jats:italic>H<\/jats:italic>\n                    .\n                    <jats:italic>parasuis<\/jats:italic>\n                    ,\n                    <jats:italic>S. suis<\/jats:italic>\n                    or\n                    <jats:italic>P. multocida<\/jats:italic>\n                    between WT PRV positive and negative farms (\n                    <jats:italic>p\u00a0&gt;\u00a0<\/jats:italic>\n                    .05). However, the detection rate of attenuated\n                    <jats:italic>H<\/jats:italic>\n                    .\n                    <jats:italic>parasuis<\/jats:italic>\n                    and\n                    <jats:italic>S. suis<\/jats:italic>\n                    strains were 68.19% and 64.75%, respectively, in WT PRV infected farms, which were significantly higher than those (41.56% and 52.25%) in WT PRV free farms (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05). The prevalent serotypes of\n                    <jats:italic>H<\/jats:italic>\n                    .\n                    <jats:italic>parasuis<\/jats:italic>\n                    \u20105\/12 and\n                    <jats:italic>S. suis<\/jats:italic>\n                    \u20102 were also investigated by multiplex PCR. These results indicated that the presence of WT PRV increased the chance of bacterial infection and the number of pathogenic strains in the respiratory system of pigs. Therefore, the eradication of pseudorabies is an effective approach to prevent and control the bacterial respiratory diseases in the intensive pig farms in China.\n                  <\/jats:p>","DOI":"10.1002\/vms3.289","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T22:04:46Z","timestamp":1593036286000},"page":"175-183","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["The epidemiological investigation of co\u2010infection of major respiratory bacteria with pseudorabies virus in intensive pig farms in China"],"prefix":"10.1002","volume":"7","author":[{"given":"Xuexiang","family":"Yu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology College of Veterinary Medicine Huazhong Agricultural University Wuhan China"},{"name":"The Cooperative Innovation Center for Sustainable Pig Production Wuhan 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