{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T14:13:09Z","timestamp":1783606389990,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,6,21]],"date-time":"2020-06-21T00:00:00Z","timestamp":1592697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Intra-ACP project","award":["2012-3166"],"award-info":[{"award-number":["2012-3166"]}]},{"name":"Intermediate Fellowship Public Health and Tropical Medicine","award":["WT104017MA to CJK"],"award-info":[{"award-number":["WT104017MA to CJK"]}]},{"name":"Tanzanian Partnership Program One Health Brucellosis","award":["DVM-039"],"award-info":[{"award-number":["DVM-039"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Brucellosis is a zoonotic disease of importance to both public health and the livestock industry. The disease is likely to be endemic in Tanzania and little is reported on molecular characterization of <jats:italic>Brucella<\/jats:italic> species in pastoral settings. This study aimed at characterizing <jats:italic>Brucella<\/jats:italic> species (targeting genus <jats:italic>Brucella<\/jats:italic>) infecting humans, cattle and goat in Kagera region (Ngara and Karagwe districts) using real\u2010time PCR, PCR amplification of 16S rRNA genes and Sanger sequencing. <jats:italic>Brucella<\/jats:italic> spp. were detected in 47 samples (19 sera and 28 milk) out of 125 samples (77 sera, 35 milk and 13 aborted materials) using real\u2010time PCR. All aborted materials (13 samples) were negative to real\u2010time PCR. Out of the 47 real\u2010time PCR positive samples (28 milk and 19 sera), 20 samples (10 milk and 10 sera) showed an expected 16S rRNA gene PCR product. Sequence analysis and blasting confirmed the presence of <jats:italic>Brucella<\/jats:italic> spp. in pastoral areas of Kagera region. The <jats:italic>Brucella<\/jats:italic> spp. from Kagera were phylogenetically grouped in two clades and three branches all closer to <jats:italic>B. melitensis<\/jats:italic>, <jats:italic>B. abortus<\/jats:italic> and <jats:italic>B. suis<\/jats:italic> from USA, Sudan and Iran. However, they were distinct from other species isolated also in USA, New Zealand, Germany and Egypt. This was expected based on the distance between the geographical regions from which the data (nucleotides sequences from 16S gene sequencing) for the phylogeny reconstruction were obtained. This is the first study to report <jats:italic>Brucella<\/jats:italic> species identified using 16S rRNA gene sequencing in East and Central Africa. A livestock vaccination program re\u2010inforced with a high index of <jats:italic>Brucella<\/jats:italic> diagnosis is needed to eradicate brucellosis in animals and minimize suffering from <jats:italic>Brucella<\/jats:italic> infections in humans in Tanzania.<\/jats:p>","DOI":"10.1002\/vms3.298","type":"journal-article","created":{"date-parts":[[2020,6,22]],"date-time":"2020-06-22T01:18:25Z","timestamp":1592788705000},"page":"711-719","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Molecular characterization of <i>Brucella<\/i> species detected in humans and domestic ruminants of pastoral areas in Kagera ecosystem, Tanzania"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3033-4047","authenticated-orcid":false,"given":"Jean\u2010Bosco","family":"Ntirandekura","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine and Biomedical Sciences Department of Veterinary Medicine and Public Health Sokoine University of Agriculture Morogoro Tanzania"},{"name":"D\u00e9partement de Sant\u00e9 et Productions Animales Facult\u00e9 d\u2019Agronomie et de Bio\u2010Ing\u00e9nierie Universit\u00e9 du Burundi Bujumbura Burundi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor A.","family":"Makene","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biotechnology The University of Dar es Salaam Dar es Salaam Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Kasanga","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Biomedical Sciences Department of Microbiology, Parasitology and Biotechnology Sokoine University of Agriculture Morogoro Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas E.","family":"Matemba","sequence":"additional","affiliation":[{"name":"The National Institute for Medical Research Dodoma Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharadhuli I.","family":"Kimera","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Biomedical Sciences Department of Veterinary Medicine and Public Health Sokoine University of Agriculture Morogoro Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John B.","family":"Muma","sequence":"additional","affiliation":[{"name":"Department of Disease Control TheUniversity of Zambia School of Veterinary Medicine Lusaka Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esron D.","family":"Karimuribo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Biomedical Sciences Department of Veterinary Medicine and Public Health Sokoine University of Agriculture Morogoro Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,21]]},"reference":[{"issue":"189","key":"e_1_2_10_2_1","first-page":"1","article-title":"Epidemiology of Brucella infection in the human, livestock and wildlife interface in the Katavi\u2010Rukwa ecosystem","volume":"11","author":"Assenga J. 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