{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T02:23:51Z","timestamp":1788143031264,"version":"build-2803163510"},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,6,17]],"date-time":"2020-06-17T00:00:00Z","timestamp":1592352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007514","name":"Universit\u00e0 di Pisa","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007514","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    is widespread in rodents, the most studied\n                    <jats:italic>reservoir<\/jats:italic>\n                    and the main hosts involved in its transmission. In Italy, among rodents,\n                    <jats:italic>Hystrix cristata<\/jats:italic>\n                    (crested porcupine) is the largest species and it is distributed all over the country. In this paper, the isolation and characterization of pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. from the kidney of\n                    <jats:italic>H. cristata<\/jats:italic>\n                    is reported for the first time. During Autumn 2018,\n                    <jats:italic>Leptospira<\/jats:italic>\n                    detection by real\u2010time PCR and isolation were performed from kidneys of two died female porcupines (an adult and a porcupette). Only for porcupette kidney sample, real\u2010time PCR for pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    tested positive. The isolated strain was identified as\n                    <jats:italic>Leptospira interrogans<\/jats:italic>\n                    serogroup Pomona serovar Pomona, using the three schemes of multilocus sequence typing. The results show that\n                    <jats:italic>H. cristata<\/jats:italic>\n                    could be a\n                    <jats:italic>Leptospira<\/jats:italic>\n                    host. The infection of serovars Pomona could be related to the habitat shared with wild boar, a typical\n                    <jats:italic>reservoir<\/jats:italic>\n                    host for this serovar.\n                  <\/jats:p>","DOI":"10.1002\/vms3.308","type":"journal-article","created":{"date-parts":[[2020,6,17]],"date-time":"2020-06-17T07:16:04Z","timestamp":1592378164000},"page":"985-991","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Isolation of\n                    <i>Leptospira<\/i>\n                    serovar Pomona from a crested porcupine (\n                    <i>Hystrix cristata<\/i>\n                    , L., 1758)"],"prefix":"10.1002","volume":"6","author":[{"given":"Giovanni","family":"Cilia","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5292-0613","authenticated-orcid":false,"given":"Fabrizio","family":"Bertelloni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1571-3620","authenticated-orcid":false,"given":"Francesca","family":"Coppola","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Turchi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Biliotti","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa Pisa Italy"},{"name":"CRASM \u201cSemproniano\u201d Grosseto Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Poli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Parisi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Felicioli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Cerri","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1717-2947","authenticated-orcid":false,"given":"Filippo","family":"Fratini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.03.012"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis035"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-0711-5-28"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.4102\/ojvr.v81i1.821"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2019.04.001"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007141"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001954"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01285-17"},{"key":"e_1_2_11_10_1","first-page":"383","article-title":"Epidemiology of leptospirosis: Observations on serological data obtained by a \u201cdiagnostic laboratory for leptospirosis\u201d from 1995 to 2001","volume":"26","author":"Cerri D.","year":"2003","journal-title":"New Microbiology"},{"key":"e_1_2_11_11_1","article-title":"Seroprevalence and risk factors of leptospirosis in wild boars (Sus scrofa) in northern Italy","volume":"27","author":"Chiari M.","year":"2016","journal-title":"Hystrix, Italian Journal of Mammalogy"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.04.022"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2020.101472"},{"key":"e_1_2_11_14_1","unstructured":"Coppola F. 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