{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T13:06:47Z","timestamp":1780578407581,"version":"3.54.1"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,8,30]],"date-time":"2020-08-30T00:00:00Z","timestamp":1598745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    An outbreak of 92 abortions out of 1,700 pregnant cows (5.41%) in a period of 3\u00a0weeks (19 May to 05 June 2019) occurred in a Georgia Dairy, USA, in cattle that were between 3 and 7\u00a0months of gestation. Two sets of samples (aborted fetuses\u2019 organs, placental tissues, aborted cows blood) were submitted for laboratory investigations at the Tifton Veterinary Diagnostic and Investigational Laboratory, College of Veterinary Medicine, University of Georgia (TVDIL, Tifton, GA, USA). An abortion panel testing for the major abortion\u2010causing agents [e.g. Bovine Viral Diarrhoea Virus (BVDV), Infectious Bovine Rhinotracheitis Virus\/ Bovine Herpes Virus\u2010I (IBR\/ BHV\u2010I),\n                    <jats:italic>Brucella spp., Leptospira spp<\/jats:italic>\n                    .] was conducted on several of the samples. On the first set of samples, microbial cultures, serology and PCR tests for the common abortifacient agents revealed the presence of\n                    <jats:italic>Neospora caninum (N. caninum)<\/jats:italic>\n                    DNA, which was positive by PCR on the placenta and fetal tissues. The second set of diagnostic investigations also identified two out of three submitted freshly aborted fetuses to be positive for\n                    <jats:italic>N. caninum<\/jats:italic>\n                    by PCR and immunohistochemistry. Moreover, all three dams were also sero\u2010positive for\n                    <jats:italic>N. caninum<\/jats:italic>\n                    . The entire herd was being fed on grass silage harvested from a pasture where feral pigs were hunted previously and carcasses were left behind. As a consequence of this action a large population of wild coyotes were attracted to these carcasses, and likely contaminated the pasture with potential\n                    <jats:italic>N. caninum<\/jats:italic>\n                    \u2010infected feces. After the abortion outbreak was resolved, it was recommended that the farmers should avoid disposal of cadavers of hunted animals in the wild, as it could attract carnivorous and omnivorous animals that may potentially spread the disease to the cattle and other wildlife.\n                  <\/jats:p>","DOI":"10.1002\/vms3.346","type":"journal-article","created":{"date-parts":[[2020,8,30]],"date-time":"2020-08-30T20:39:36Z","timestamp":1598819976000},"page":"141-147","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["An outbreak of\n                    <i>Neospora caninum<\/i>\n                    abortion in a dairy herd from the State of Georgia, United States"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7217-9244","authenticated-orcid":false,"given":"Pedro","family":"Melendez","sequence":"first","affiliation":[{"name":"Department of Population Health College of Veterinary Medicine University of Georgia Tifton GA USA"},{"name":"Tifton Veterinary Diagnostic and Investigational Laboratory College of Veterinary Medicine University of Georgia Tifton GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcia","family":"Ilha","sequence":"additional","affiliation":[{"name":"Tifton Veterinary Diagnostic and Investigational Laboratory College of Veterinary Medicine University of Georgia Tifton GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moges","family":"Woldemeskel","sequence":"additional","affiliation":[{"name":"Tifton Veterinary Diagnostic and Investigational Laboratory College of Veterinary Medicine University of Georgia Tifton GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin","family":"Graham","sequence":"additional","affiliation":[{"name":"LeeCo Dairy Lee County GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Coarsey","sequence":"additional","affiliation":[{"name":"Tifton Veterinary Diagnostic and Investigational Laboratory College of Veterinary Medicine University of Georgia Tifton GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debi","family":"Baughman","sequence":"additional","affiliation":[{"name":"Tifton Veterinary Diagnostic and Investigational Laboratory College of Veterinary Medicine University of Georgia Tifton GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Whittington","sequence":"additional","affiliation":[{"name":"Tifton Veterinary Diagnostic and Investigational Laboratory College of Veterinary Medicine University of Georgia Tifton GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3221-3222","authenticated-orcid":false,"given":"Hemant","family":"Naikare","sequence":"additional","affiliation":[{"name":"Tifton Veterinary Diagnostic and Investigational Laboratory College of Veterinary Medicine University of Georgia Tifton GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-018-1390-x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2019.04.002"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870802000306"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-07-0065"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(02)00094-2"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00031-06"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(94)77212-X"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13358"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(00)00373-3"},{"key":"e_1_2_9_11_1","volume-title":"Fluorescent antibody techniques and their applications","author":"Kawamura A.","year":"1977"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2010.01.008"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.7589\/2018-02-027"},{"key":"e_1_2_9_14_1","volume-title":"Diagnostic procedures for viral, rickettsial and chlamydial infections","author":"Lennette E. H.","year":"1979"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2019.11.004"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(99)00054-0"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.05.010"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2017.07.022"},{"key":"e_1_2_9_19_1","volume-title":"Clinical veterinary microbiology","author":"Quinn P. J.","year":"1994"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2012.10.022"},{"key":"e_1_2_9_21_1","unstructured":"World Health Organization(2019).https:\/\/www.who.int\/environmental_health_emergencies\/disease_outbreaks\/en\/."},{"key":"e_1_2_9_22_1","unstructured":"World Organisation for Animal Health. (2019).Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. (2019). Leptospirosis.https:\/\/www.oie.int\/standard\u2010setting\/terrestrial\u2010manual\/access\u2010online."}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.346","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vms3.346","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.346","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T14:22:56Z","timestamp":1693750976000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,30]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1002\/vms3.346"],"URL":"https:\/\/doi.org\/10.1002\/vms3.346","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.346\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.346\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.346\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.346\/v2\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.346\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.346\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.346\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.346\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.346\/v2\/decision1","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,30]]},"assertion":[{"value":"2020-02-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}