{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:15:54Z","timestamp":1772118954341,"version":"3.50.1"},"reference-count":11,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,9,26]],"date-time":"2020-09-26T00:00:00Z","timestamp":1601078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study describes a simple method for the large\u2010scale isolation of pure\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    tachyzoites and bradyzoites.\n                    <jats:italic>T. gondii<\/jats:italic>\n                    tachyzoites were obtained from infected human foreskin fibroblasts (HFFs) and peritoneal exudates of mice, while tissue cysts containing bradyzoites were collected from chronically infected mice. Harvested cells and brain tissues were incubated in Hanks balanced salt solution (HBSS), containing 0.25% trypsin and 0.5% taurodeoxycholic acid (TDC) for 5\u00a0min. Subsequent washes in phosphate buffered saline (PBS) were conducted, and the cell viability of the preparations was good, as determined by flow cytometry and ability to reinfect HFF cells and propagate in mice. The purification procedure allowed for a rapid preparation of pure\n                    <jats:italic>T. gondii<\/jats:italic>\n                    tachyzoites and bradyzoites in sufficient quantity that can be used for downstream procedures. The advantage of the new method is that it is convenient and inexpensive.\n                  <\/jats:p>","DOI":"10.1002\/vms3.364","type":"journal-article","created":{"date-parts":[[2020,9,26]],"date-time":"2020-09-26T15:34:37Z","timestamp":1601134477000},"page":"357-361","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A rapid and simple single\u2010step method for the purification of\n                    <i>Toxoplasma gondii<\/i>\n                    tachyzoites and bradyzoites"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0389-1980","authenticated-orcid":false,"given":"Saeed","family":"El\u2010Ashram","sequence":"first","affiliation":[{"name":"College of Life Science and Engineering Foshan University Foshan Guangdong Province China"},{"name":"Faculty of Science Kafrelsheikh University Kafr el\u2010Sheikh 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