{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T18:50:29Z","timestamp":1768675829627,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T00:00:00Z","timestamp":1600905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010679","name":"Pasteur Institute of Iran","doi-asserted-by":"publisher","award":["973"],"award-info":[{"award-number":["973"]}],"id":[{"id":"10.13039\/501100010679","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Zoonotic Cutaneous Leishmaniasis is increasing in the world and <jats:italic>Phlebotomus papatasi<\/jats:italic> as a proven vector was considered in different aspects for disease control. Sandfly saliva contains proteins which provoke host immune system. These proteins are candidates for developing vaccines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The main purpose of this research was comparing evaluation of salivary glands proteomes from wild <jats:italic>P. papatasi<\/jats:italic>. Extracting these proteins and purifying of original SP15 as inducer agent in vector salivary glands from endemic leishmaniasis foci were other objectives.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Adult sandflies were sampled using aspirators and funnel traps from three endemic foci in 2017\u20132018. Each pair of salivary glands of unfed females was dissected and proteins were extracted using thermal shocking and sonication methods. Purification was performed through RP\u2010HPLC. All equivalent fractions were added together in order to reach sufficient protein concentration. Protein content and profile determination were examined with SDS\u2010PAGE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The protein concentration of whole\u2010salivary glands of specimens was determined approximately 1.6\u00a0\u00b5g\/\u00b5l (Isfahan) and 1\u00a0\u00b5g\/\u00b5l (Varamin and Kashan). SDS\u2010PAGE revealed 10 distinct bands between 10 and 63\u00a0kDa. Analysis of proteomes showed some similarities and differences in the chromatograms of different foci. SDS\u2010PAGE of all collected fractions revealed SP15\u2010like proteins were isolated in 24\u00a0min from Varamin, 26 to 30\u00a0min from Kashan and 29.4\u00a0min from Isfahan and were around 15\u00a0kDa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Isolation of salivary components of Iranian wild <jats:italic>P. papatasi<\/jats:italic> is very important for finding potential proteins in vaccine development and measuring control strategy of zoonotic cutaneous leishmaniasis in Iran and this could be concluded elsewhere in the world.<\/jats:p><\/jats:sec>","DOI":"10.1002\/vms3.368","type":"journal-article","created":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T11:38:33Z","timestamp":1600947513000},"page":"362-369","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Comparative evaluation of salivary glands proteomes from wild <i>Phlebotomus papatasi<\/i><i>\u2013<\/i>proven vector of zoonotic cutaneous leishmaniasis in Iran"],"prefix":"10.1002","volume":"7","author":[{"given":"Seyedeh","family":"Maryam Ghafari","sequence":"first","affiliation":[{"name":"Molecular Systematics Laboratory Parasitology Department Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sahar","family":"Ebrahimi","sequence":"additional","affiliation":[{"name":"Molecular Systematics Laboratory Parasitology Department Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmoud","family":"Nateghi Rostami","sequence":"additional","affiliation":[{"name":"Parasitology Department Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Bordbar","sequence":"additional","affiliation":[{"name":"Molecular Systematics Laboratory Parasitology Department Pasteur Institute of Iran Tehran 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