{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T15:53:48Z","timestamp":1781711628778,"version":"3.54.5"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T00:00:00Z","timestamp":1609286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>African swine fever (ASF) is a highly contagious fatal infectious disease of pigs and wild suids. The disease has a worldwide occurrence and significant impact on pig production. Two adult intensively raised large white boars from two farms in Jos with a history of sudden death were diagnosed of ASF between July and August 2019. Post\u2010mortem examination of carcasses grossly showed splenomegaly, haemorrhagic lymphadenitis and hepatomegaly with severe congestion. The kidneys were enlarged and had generalized petechiae and blood clot in the pelvis. The heart was moderately enlarged. Microscopic examination of the spleen and lymph nodes revealed severe lymphocytic depletion, haemorrhage and severe haemosiderosis. The liver was severely congested with focal coagulative necrosis of the hepatocytes. The kidneys were severely congested and showed renal tubular necrosis with few tubular protein casts. Tissue samples were confirmed to be positive for African swine fever virus (ASFV) by polymerase chain reaction (PCR) assay, and phylogenetic analysis revealed that the isolate belonged to genotype I.<\/jats:p>","DOI":"10.1002\/vms3.403","type":"journal-article","created":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T22:49:20Z","timestamp":1609368560000},"page":"705-713","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Laboratory diagnosis of a new outbreak of acute African swine fever in smallholder pig farms in Jos, Nigeria"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0361-1902","authenticated-orcid":false,"given":"Emmanuel V.","family":"Tizhe","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology Faculty of Veterinary Medicine University of Jos Jos Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pam D.","family":"Luka","sequence":"additional","affiliation":[{"name":"National Veterinary Research Institute Vom Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5736-6466","authenticated-orcid":false,"given":"Adeyinka J.","family":"Adedeji","sequence":"additional","affiliation":[{"name":"National Veterinary Research Institute Vom Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Polycarp","family":"Tanko","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology Faculty of Veterinary Medicine University of Jos Jos Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George Y.","family":"Gurumyen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology Faculty of Veterinary Medicine University of Jos Jos Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah M.","family":"Buba","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology Faculty of Veterinary Medicine University of Jos Jos Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ussa D.","family":"Tizhe","sequence":"additional","affiliation":[{"name":"Department of Medicine Faculty of Veterinary Medicine Ahmadu Bello University Zaria Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asinamai A.","family":"Bitrus","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology Faculty of Veterinary Medicine University of Jos Jos Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur O.","family":"Oragwa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology Faculty of Veterinary Medicine University of Jos Jos Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samson J.","family":"Shaibu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology Faculty of Veterinary Medicine University of Jos Jos Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Essieniffiok S.","family":"Unanam","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Surgery and Radiology Faculty of Veterinary Medicine University of Jos Jos Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ikechukwu O.","family":"Igbokwe","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology Faculty of Veterinary Medicine University of Maiduguri Maiduguri Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen O.","family":"Akpavie","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology Faculty of Veterinary Medicine University of Ibadan Ibadan Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celestine O.","family":"Njoku","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology Faculty of Veterinary Medicine University of Jos Jos Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,30]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"199","volume-title":"Introduction to medical laboratory technology","author":"Baker J.","year":"2000"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2009.0098"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-animal-021419-083741"},{"key":"e_1_2_10_5_1","unstructured":"European Union Reference Laboratory for African Swine Fever. 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