{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:15:54Z","timestamp":1772118954276,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,12,13]],"date-time":"2020-12-13T00:00:00Z","timestamp":1607817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Atypical porcine pestivirus (APPV) was identified and associated with congenital tremor (CT) type A\u2010II in new born piglets and has been reported in many countries. In China, the first APPV identification in swine herds was reported in Guangdong province in 2016. To investigate the genetic characteristics of APPV in Guangxi province, 53\u00a0tissue samples from neonatal piglets with CT were collected and detected from October 2017 to May 2019. Five APPV strains which were named as GX04\/2017, GX01\u20102018, GX02\u20102018, GX01\u20102019 and GX02\u20102019 were obtained. Sequence analysis revealed that all six APPV strains from Guangxi province, including five strains from this study and one from a previous report, shared 83.3%\u201097.5% nucleotide identity of complete genome and 91.7%\u201099.1% amino acid identity of the open reading frame (ORF), and shared 77.7%\u201097.7% nucleotide identity of complete genome and 90.6%\u201099.3% amino acid identity of ORF with reference strains. Phylogenetic analysis indicated that all APPV strains could be divided into three clades based on the complete genome, N\n                    <jats:sup>pro<\/jats:sup>\n                    , E\n                    <jats:sup>rns<\/jats:sup>\n                    and E2 gene sequences, respectively; and the APPV strains from Guangxi province distributed in two clades (clades I and II). No sign of recombination was observed from Guangxi strains. Evolution analysis performed on the complete genome of 58 APPV strains showed that America, Europe and Asia strains during 2006\u20132019 evolved at a mean rate of 1.37\u00a0\u00d7\u00a010\n                    <jats:sup>\u20134<\/jats:sup>\n                    substitutions\/site\/year, and the most recent common ancestor (tMRCA) of them was estimated as 1,700.5\u00a0years ago. The findings of this study indicated that there existed a high degree of genetic diversity of APPV from Guangxi province, Southern China, which provided important information on the epidemiological features and evolutionary relationships of APPV.\n                  <\/jats:p>","DOI":"10.1002\/vms3.407","type":"journal-article","created":{"date-parts":[[2020,12,13]],"date-time":"2020-12-13T07:47:07Z","timestamp":1607845627000},"page":"714-723","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Evolution and genetic diversity of atypical porcine pestivirus (APPV) from piglets with congenital tremor in Guangxi Province, Southern China"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1023-9635","authenticated-orcid":false,"given":"Kaichuang","family":"Shi","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Guangxi University Nanning China"},{"name":"Guangxi Center for Animal Disease Control and Prevention Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shouyu","family":"Xie","sequence":"additional","affiliation":[{"name":"Guangxi Center for Animal Disease Control and Prevention Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0805-6781","authenticated-orcid":false,"given":"Wenchao","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Virology Wenzhou University Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huixin","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Guangxi University Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Guangxi University Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanwen","family":"Yin","sequence":"additional","affiliation":[{"name":"Guangxi Center for Animal Disease Control and Prevention Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongbin","family":"Si","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Guangxi University Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sujie","family":"Qu","sequence":"additional","affiliation":[{"name":"Guangxi Center for Animal Disease Control and Prevention Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjun","family":"Lu","sequence":"additional","affiliation":[{"name":"Guangxi Center for Animal Disease Control and Prevention Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-018-3962-z"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0150104"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12532"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0166863"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12854"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2017.06.024"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12060680"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/v8100271"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12981"},{"key":"e_1_2_9_11_1","first-page":"429","article-title":"First report of atypical porcine pestivirus in piglets with congenital tremor in Canada","volume":"59","author":"Dessureault F. 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