{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:15:44Z","timestamp":1772118944645,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,1,2]],"date-time":"2021-01-02T00:00:00Z","timestamp":1609545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The field efficacy of a bivalent vaccine containing porcine circovirus type 2b (PCV2b) and\n                      <jats:italic>Mycoplasma hyopneumoniae<\/jats:italic>\n                      was evaluated on three pig farms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Three pig farms were used, two of which had a history of subclinical PCV2 and clinical\n                      <jats:italic>M. hyopneumoniae<\/jats:italic>\n                      infections between 84 and 126\u00a0days of age while concurrent porcine circovirus\u2010associated disease and clinical\n                      <jats:italic>M. hyopneumoniae<\/jats:italic>\n                      infection between 70 and 105\u00a0days of age. Each farm vaccinated pigs with a single dose of a bivalent vaccine at 10\u00a0days of age while unvaccinated pigs were administered a single dose of phosphate buffered\u2010saline at the same age.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Vaccination improved growth performance and reduced clinical scores significantly (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a0.05) when compared with unvaccinated animals. The amount of PCV2d loads in blood and\n                      <jats:italic>M. hyopneumoniae<\/jats:italic>\n                      loads in nasal swabs of vaccinated animals were also significantly lower (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a0.05) when compared with unvaccinated animals. Immunologically, vaccinated groups elicited a significantly higher (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a0.05) level of protective immunity against PCV2d such as neutralizing antibodies and interferon\u2010\u03b3 secreting cells (IFN\u2010\u03b3\u2010SC), as well as protective immunity against\n                      <jats:italic>M. hyopneumoniae<\/jats:italic>\n                      such as IFN\u2010\u03b3\u2010SC when compared with unvaccinated animals. Pathologically, vaccination significantly lowered (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a0.05) the scores of\n                      <jats:italic>M. hyopneumoniae<\/jats:italic>\n                      \u2010induced pneumonia and PCV2\u2010associated lymphoid lesions when compared with unvaccinated animals.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The evaluated bivalent vaccine provided good protection against PCV2d and\n                      <jats:italic>M. hyopneumoniae<\/jats:italic>\n                      infection under field conditions.\n                    <\/jats:p>\n                  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