{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T02:52:45Z","timestamp":1787626365429,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T00:00:00Z","timestamp":1620086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004320","name":"Shiraz University of Medical Sciences","doi-asserted-by":"publisher","award":["95\u201001\u201043\u201012905"],"award-info":[{"award-number":["95\u201001\u201043\u201012905"]}],"id":[{"id":"10.13039\/501100004320","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Canine visceral leishmaniasis (CVL) is endemic in the southern parts of Iran. The current study aimed at molecular and serological evaluation of zoonotic visceral leishmaniasis in dogs in Fars province, southern Iran. Blood samples were collected from 60 dogs in the three villages in Fars Province. Serum samples were tested for antibodies against\n                    <jats:italic>L. infantum<\/jats:italic>\n                    by direct agglutination test (DAT). DNA was extracted from each dog's buffy coat and tested by PCR, targeting the\n                    <jats:italic>Leishmania<\/jats:italic>\n                    ITS\u20102 region. From a total of 60 studied dogs, 25 (41.7%) were female, and 35 (58.3%) were male. Dogs' age ranged from 1 to 7\u00a0years, with a mean age of 2.97 (\u00b11.4) years. Anti\u2010\n                    <jats:italic>Leishmania<\/jats:italic>\n                    antibodies were detected in sera samples of 28 (46.7%) dogs, (titre\u00a0\u2265\u00a01:320). Out of 28 seropositive cases, 13 (46.4%) were female, and 15 (53.6%) were male. Association between seropositivity and dogs\u2019 clinical signs was statistically significant (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05).\n                    <jats:italic>Leishmania<\/jats:italic>\n                    DNA was detected in the buffy coat of 3 of 60 studied dogs which were all seropositive by DAT. The PCR products were sequenced and molecular analysis showed that two of the isolates were\n                    <jats:italic>Leishmania infantum,<\/jats:italic>\n                    and one was\n                    <jats:italic>L. tropica<\/jats:italic>\n                    . The high proportion of seropositive dogs indicates the important role of these animals in the epidemiology of the disease in the region. Infected dogs with or without signs, especially those that are molecularly positive, can act as an active reservoir of the disease in the area.\n                  <\/jats:p>","DOI":"10.1002\/vms3.432","type":"journal-article","created":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T05:36:08Z","timestamp":1620106568000},"page":"1082-1089","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Molecular and serological evaluation of zoonotic visceral leishmaniasis in dogs in a rural area of Fars province, southern Iran, as a source of\n                    <i>Leishmania infantum<\/i>\n                    infection"],"prefix":"10.1002","volume":"7","author":[{"given":"Laleh","family":"Najafi","sequence":"first","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mostafa","family":"Omidian","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra","family":"Rezaei","sequence":"additional","affiliation":[{"name":"Professor Alborzi Clinical Microbiology Research Center Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeed","family":"Shahabi","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fariba","family":"Ghorbani","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasir","family":"Arefkhah","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Yasuj University of Medical Sciences Yasuj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Mohebali","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Mycology School of Public Health Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zabiolla","family":"Zaraei","sequence":"additional","affiliation":[{"name":"Meshkin\u2010Shahr Health Station School of Public Health Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2045-9057","authenticated-orcid":false,"given":"Bahador","family":"Sarkari","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz Iran"},{"name":"Basic Sciences in Infectious Diseases Research Center Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2008.79.839"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2006.74.306"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.08.006"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-2050-7"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1309.070101"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.7205\/MILMED.170.4S.17"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1179\/136485910X12786389891083"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1203.050811"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.27.10.2252-2257.1989"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00580-011-1179-6"},{"key":"e_1_2_10_12_1","first-page":"51","article-title":"Molecular and seroepidemiological survey of visceral leishmaniasis among humans and domestic dogs in Mazandaran Province, north of Iran","volume":"6","author":"Fakhar M.","year":"2011","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1997.57.172"},{"key":"e_1_2_10_14_1","first-page":"152","article-title":"Seroepidemiologcal investigation of visceral leishmaniasis in stray and owned dogs in alborz province, central iran using direct agglutination test","volume":"8","author":"Haddadzade H.","year":"2013","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_10_15_1","first-page":"85","article-title":"Leishmania tropica: Another etiological agent of canine visceral leishmaniasis in Iran","volume":"36","author":"Hajaran H.","year":"2007","journal-title":"Iranian Journal of Public Health"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2009.0023"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.26719\/2010.16.3.340"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1865-1682.2011.01209.x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.2.613-617.2001"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2013.03.017"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/8167247"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-014-8103-2"},{"key":"e_1_2_10_23_1","first-page":"2161","article-title":"Epidemiological status of visceral leishmaniasis in Iran: Experiences and review of literature","volume":"3","author":"Mohebali M.","year":"2012","journal-title":"Journal of Clinical and Experimental Pathology"},{"issue":"3","key":"e_1_2_10_24_1","first-page":"348","article-title":"Visceral leishmaniasis in Iran: Review of the epidemiological and clinical features","volume":"8","author":"Mohebali M.","year":"2013","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_10_25_1","first-page":"15","article-title":"Application of direct agglutination test (DAT) for the diagnosis and seroepide\u2010miological studies of visceral leishmaniasis in Iran","volume":"1","author":"Mohebali M.","year":"2006","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2005.01.010"},{"key":"e_1_2_10_27_1","first-page":"69","article-title":"Disseminated leishmaniasis caused by Leishmania tropica in a puppy from Karaj, central Iran","volume":"6","author":"Mohebali M.","year":"2011","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vprsr.2017.03.010"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.4103\/0972-9062.249125"},{"issue":"3","key":"e_1_2_10_30_1","first-page":"1","article-title":"Seroepidemiological study on canine visceral leishmaniasis in Meshkin\u2010Shahr district, Ardabil province, northwest of Iran during 2006\u20132007","volume":"3","author":"Moshfe A.","year":"2008","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2009.07.004"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2014.09.002"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(02)02296-1"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.2.748-751.2000"},{"issue":"1","key":"e_1_2_10_35_1","first-page":"126","article-title":"Molecular evaluation of a case of visceral leishmaniasis due to Leishmania tropica in Southwestern Iran","volume":"11","author":"Sarkari B.","year":"2016","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12639-013-0393-3"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1179\/136485909X398276"},{"key":"e_1_2_10_38_1","first-page":"94","article-title":"Epidemiological features of visceral leishmaniasis in Fars province, southern Iran","volume":"41","author":"Sarkari B.","year":"2012","journal-title":"Iranian Journal of Public Health"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0150406"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(03)00093-2"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2016.08.020"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2011.06.003"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.432","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vms3.432","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.432","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T07:48:35Z","timestamp":1693295315000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.432"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,4]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1002\/vms3.432"],"URL":"https:\/\/doi.org\/10.1002\/vms3.432","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.432\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.432\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.432\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.432\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.432\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.432\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.432\/v1\/decision1","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,4]]},"assertion":[{"value":"2020-07-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}