{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:55:45Z","timestamp":1785200145544,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,2,24]],"date-time":"2021-02-24T00:00:00Z","timestamp":1614124800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Fascioliasis is a parasitic infection caused by\n                    <jats:italic>Fasciola<\/jats:italic>\n                    spp. in humans and animals. Despite significant advances in vaccination and new therapeutic agents, little attention has been paid to validate methods for the diagnosis of fascioliasis in animals. This study aimed to compare the loop\u2010mediated isothermal amplification (LAMP) technique with PCR assay for the diagnosis of\n                    <jats:italic>F. hepatica<\/jats:italic>\n                    in sheep. In this cross\u2010sectional study, 195 stool samples were collected from sheep for 3\u00a0months in Lorestan province, West of Iran. Specimens\u2019 parasitological examination was performed by using the direct wet mount and formalin\u2010ether concentration method. After DNA extraction from the samples, molecular analysis was done using PCR and LAMP techniques based on the\n                    <jats:italic>Fasciola<\/jats:italic>\n                    ribosomal intergenic spacer (IGS) sequence. Of 195 specimens of sheep, 11 specimens were identified as\n                    <jats:italic>F. hepatica<\/jats:italic>\n                    \u2010positive infection by using microscopic, PCR and LAMP assays. Kappa agreement test results showed that there was a significant agreement between the results of microscopic examination diagnostic tests, PCR and LAMP (Kappa\u00a0=\u00a00.51\u20130.72 and\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.001). According to the results of chi\u2010square comparisons between parasite prevalence applying different techniques and variables of age, sex breed, and type of drinking water, there was no significant relationship (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0\u2265\u00a0.05). However, most of the infected sheep with\n                    <jats:italic>Fasciola<\/jats:italic>\n                    were 3\u2010 to 4\u2010year\u2010old females, of the Lori breed and consumed tap water. In many endemic areas, successful prevention and treatment of fascioliasis in animals depend on rapid and accurate diagnosis. Based on the results of the Kappa agreement, the significant agreement among the results of the microscopic examination, PCR and LAMP indicates the accuracy and reliability of these tests in the diagnosis of\n                    <jats:italic>F. hepatica<\/jats:italic>\n                    in sheep. However, molecular methods, especially the LAMP technique, are suggested because of their higher sensitivity and reliability for the diagnosis of\n                    <jats:italic>F. hepatica<\/jats:italic>\n                    even under field conditions.\n                  <\/jats:p>","DOI":"10.1002\/vms3.455","type":"journal-article","created":{"date-parts":[[2021,2,24]],"date-time":"2021-02-24T06:04:11Z","timestamp":1614146651000},"page":"1316-1324","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Accurate and rapid detection of\n                    <i>Fasciola hepatica<\/i>\n                    copro\u2010DNA in sheep using loop\u2010mediated isothermal amplification (LAMP) technique"],"prefix":"10.1002","volume":"7","author":[{"given":"Siamak","family":"Amiri","sequence":"first","affiliation":[{"name":"Department of Pathobiology, Science and Research branch Islamic Azad University Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahar","family":"Shemshadi","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, Science and Research branch Islamic Azad University Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saloomeh","family":"Shirali","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, Science and Research branch Islamic Azad University Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farnaz","family":"Kheirandish","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Lorestan University of Medical Sciences Khorramabad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1826-7910","authenticated-orcid":false,"given":"Shirzad","family":"Fallahi","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Lorestan University of Medical Sciences Khorramabad Iran"},{"name":"Hepatitis Research Center Lorestan University of Medical Sciences Khorramabad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.09.005"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-800182-0.00002-7"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2011.08.009"},{"key":"e_1_2_10_5_1","first-page":"306","article-title":"The status of human and animal Fascioliasis in Iran: A narrative review article","volume":"10","author":"Ashrafi K.","year":"2015","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_10_6_1","first-page":"1","article-title":"Fasciola hepatica in some buffaloes and cattle by PCR and microscopy","volume":"2","author":"Ayaz S.","year":"2014","journal-title":"Science World Journal"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3923\/pjbs.2009.1212.1216"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1477-9552.2005.tb00126.x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-1812-3"},{"key":"e_1_2_10_10_1","unstructured":"CDC. 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