{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:57:51Z","timestamp":1776387471672,"version":"3.51.2"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T00:00:00Z","timestamp":1620000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["283804"],"award-info":[{"award-number":["283804"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Differences in total number of piglets born per litter are observed between the Norwegian Duroc (ND) sire and Norwegian Landrace (NL) dam line. The aim of this study was to evaluate ovarian characteristics, and in vitro nuclear and cytoplasmic oocyte maturation in both breeds. One day after weaning, follicular phase ovaries were collected. Ovary length and weight were measured and the number of follicles (&lt; 3\u00a0mm and 3\u20138\u00a0mm) was counted. Cumulus\u2010oocyte complexes (COCs) were collected and matured for 48\u00a0hr. To assess cumulus expansion, COC area was analysed at 0 and 20\u00a0hr. Nuclear maturation and cortical granule (CG) distribution were analysed at 20 and 48\u00a0hr, and total glutathione (GSH) was measured at 48\u00a0hr to further elucidate cytoplasmic maturation. In first parity sows, a smaller ovary length and fewer 3 to 8\u00a0mm follicles were observed in ND compared to NL. For all sows, ND COCs covered a significantly smaller area at 0\u00a0hr, but a higher cumulus expansion ratio was observed at 20\u00a0hr compared to NL (364\u00a0\u00b1\u00a046% versus. 278\u00a0\u00b1\u00a027%, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). At 20\u00a0hr, more ND oocytes exhibited advanced stages of nuclear maturation, while more NL oocytes showed advanced stages of CG distribution. Nuclear maturation to MII stage at 48\u00a0hr did not differ between ND and NL oocytes (90.1% and 87.7%, respectively). Moreover, no significant differences were observed for GSH content or CG distribution after maturation. In conclusion, differences with regard to ovarian characteristics as well as to cumulus expansion, and nuclear and cytoplasmic oocyte maturation at 20\u00a0hr were observed between the breeds. Further studies are required to determine if this subsequently affects in vitro fertilization and embryo development.<\/jats:p>","DOI":"10.1002\/vms3.498","type":"journal-article","created":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T08:09:21Z","timestamp":1620029361000},"page":"1845-1853","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Ovarian characteristics and in vitro nuclear and cytoplasmic oocyte maturation in Duroc and Landrace pigs"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9991-3496","authenticated-orcid":false,"given":"Reina","family":"Jochems","sequence":"first","affiliation":[{"name":"Norsvin SA Hamar Norway"},{"name":"Faculty of Veterinary Medicine Norwegian University of Life Sciences Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8222-5056","authenticated-orcid":false,"given":"Ann Helen","family":"Gaustad","sequence":"additional","affiliation":[{"name":"Norsvin SA Hamar Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4486-6255","authenticated-orcid":false,"given":"Louisa J.","family":"Zak","sequence":"additional","affiliation":[{"name":"Topigs Norsvin Research Center Beuningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0407-0279","authenticated-orcid":false,"given":"Eli","family":"Grindflek","sequence":"additional","affiliation":[{"name":"Norsvin SA Hamar Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2248-7257","authenticated-orcid":false,"given":"Teklu T.","family":"Zeremichael","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Inland Norway University of Applied Sciences Hamar Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9702-9939","authenticated-orcid":false,"given":"Irma C.","family":"Oskam","sequence":"additional","affiliation":[{"name":"The Animal Production Experimental Centre Norwegian University of Life Sciences \u00c5s Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7873-734X","authenticated-orcid":false,"given":"Fr\u00f8ydis D.","family":"Myromslien","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Inland Norway University of Applied Sciences Hamar Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5867-4815","authenticated-orcid":false,"given":"Elisabeth","family":"Kommisrud","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Inland Norway University of Applied Sciences Hamar Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6280-5697","authenticated-orcid":false,"given":"Anette K.","family":"Krogen\u00e6s","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Norwegian University of Life Sciences Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00670-7"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(98)00180-0"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.09.023"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD07018"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:2000111"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.17085"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioz175"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1120110311"},{"issue":"111","key":"e_1_2_11_10_1","article-title":"Relationship between the estimated breeding values for litter traits at birth and ovarian and embryonic traits and their additive genetic variance in gilts at 35 days of pregnancy","volume":"9","author":"Da Silva C. 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